Heterologous biosynthesis of prenylated resveratrol through multiplex metabolic engineering in <i>Escherichia coli</i>

Wang, Haijiao; Zhou, Ting; Liu, Hui; Wen, Lingrong; Jiang, Yueming; Yang, Bao

doi:10.1039/d4gc00053f

Cited by 1 publication

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“…Since the backbone of flavonoid is based on a 15-carbon skeleton with two phenyl rings connected by a heterocyclic ring (ring C), the biosynthetic pathway of diversified flavonoids requires various types of functional enzymes. The modified derivatives of flavonoids are synthesized by methylation, hydroxylation, glycosylation, isoprenylation, and halogenation [63,64]. The diversified flavonoids showed improved bioavailability compared with flavonoid aglycone.…”

Section: Improving Intracellular Udp-sugar Level For Biosynthesis Of ...mentioning

confidence: 99%

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

Chu,

Tran,

Pham

et al. 2024

Molecules

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Flavonoids and stilbenoids, crucial secondary metabolites abundant in plants and fungi, display diverse biological and pharmaceutical activities, including potent antioxidant, anti-inflammatory, and antimicrobial effects. However, conventional production methods, such as chemical synthesis and plant extraction, face challenges in sustainability and yield. Hence, there is a notable shift towards biological production using microorganisms like Escherichia coli and yeast. Yet, the drawbacks of using E. coli and yeast as hosts for these compounds persist. For instance, yeast’s complex glycosylation profile can lead to intricate protein production scenarios, including hyperglycosylation issues. Consequently, Corynebacterium glutamicum emerges as a promising alternative, given its adaptability and recent advances in metabolic engineering. Although extensively used in biotechnological applications, the potential production of flavonoid and stilbenoid in engineered C. glutamicum remains largely untapped compared to E. coli. This review explores the potential of metabolic engineering in C. glutamicum for biosynthesis, highlighting its versatility as a cell factory and assessing optimization strategies for these pathways. Additionally, various metabolic engineering methods, including genomic editing and biosensors, and cofactor regeneration are evaluated, with a focus on C. glutamicum. Through comprehensive discussion, the review offers insights into future perspectives in production, aiding researchers and industry professionals in the field.

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Section: Improving Intracellular Udp-sugar Level For Biosynthesis Of ...mentioning

confidence: 99%

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

Chu,

Tran,

Pham

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

Heterologous biosynthesis of prenylated resveratrol through multiplex metabolic engineering in Escherichia coli

Abstract: Prenylated stilbenoids possess a range of biological activities, including anti-inflammatory, antioxidant, antidiabetic, anticancer, neuroprotective and vasorelaxant properties. However, their potential is limited by the scarcity of these compounds in nature....

Cited by 1 publication

References 48 publications

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

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