Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Chayjarung, Phadtraphorn; Poonsap, Wannakan; Pankaew, Chanyanut; Inmano, Onrut; Kongbangkerd, Anupan; Limmongkon, Apinun

doi:10.1007/s11240-021-02112-4

Cited by 17 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pre-soaking of chickpea seeds with 30 mM H 2 O 2 for 24 hr prior to germination revealed that after 72 hr, there were higher levels of total phenolic compounds and antioxidant activity compared to the control group (León-López et al, 2020). The current results were consistent another report on elicitor treatment in peanut hairy root culture that demonstrated an increase in ABTS antioxidant activity following CHT, MeJA, CD or CHT+MeJA+CD treatments (Chayjarung et al, 2021). However, the antioxidant activity detected in the treated peanut sprouts was much lower than in the hairy root culture system.…”

Section: Discussionsupporting

confidence: 92%

“…Batch-fed V. vinifera cell suspension culture treated with 50 mg/L of chitosan increased mono-glucosylated stilbene production (Ferri et al, 2011). Another study on hairy root peanut culture demonstrated the promising outcome of enhancing trans-resveratrol, trans-arachidin-1 and trans-arachidin-3 with high antioxidant activity using elicitor treatments of CHT, MeJA, CD and simultaneously CHT + MeJA + CD (Chayjarung et al, 2021). The use of an external elicitor treatment could be a promising strategy for increasing the production of phytochemicals and bioactive compounds in plants.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the purpose of the current study was to investigate the possible use of various elicitors in the priming treatment to improve stilbene compounds, especially trans-arachidin-1 and trans-arachidin-3 of peanut sprouts. The exogenous elicitors CHT, MeJA, CD and a combination of all three (CHT+MeJA+CD) improved the stilbene compounds in peanut hairy root culture (Chayjarung et al, 2021), when applied as priming substances in the peanut germination process. The current study investigated the antioxidant activity, total phenolic compounds, stilbene content and the relevant biochemical substance profiles in peanut sprout crude extract to provide a perspective on the nutritional benefits and improvement of peanut sprouts as potent functional food.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improvement of stilbene constituents and nutritional quality of peanut sprouts using abiotic elicitation and germination

2023

ANRES

View full text Add to dashboard Cite

Article InfoImportance of the work: Elicitor treatment has been widely used to improve bioactive compounds in plants. However, very few studies have reported the use of an elicitor treatment in the germination process to improve peanut quality. Objectives: To evaluate the stilbene compounds and bioactivity of peanut sprouts after seed priming using different elicitors. Materials & Methods: Peanut seeds were primed using various elicitors: chitosan (CHT), methyl jasmonate (MeJA), methyl-β-cyclodextrin (CD) or a combination of all three (CHT+MeJA+CD). Germination was carried out for 3 d. The antioxidant activity, total phenolic compound, stilbene content and the biochemical profiles in the peanut sprouts were investigated. Results: After germinating for 1-3 d, the antioxidant activities of all elicitor-treated peanut samples exhibited 1.46-2.12-fold increases compared to the untreated control. The highest contents of trans-resveratrol were produced by the peanuts treated with CHT and CHT+MeJA+CD, with 7.72 and 11.48-fold increases, respectively, compared to the untreated control. The highest contents of trans-arachidin-1 and trans-arachidin-3 were in the peanuts treated with CHT, MeJA and CHT+MeJA+CD on day 3 of germination. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile indicated the protein pattern transformed from a high molecular weight (MW) to a low MW during germination. Main finding: Combining elicitor priming and germination approaches proved to be an effective strategy that improved the phytochemical profile of peanut sprouts for functional food applications.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of stilbene constituents and nutritional quality of peanut sprouts using abiotic elicitation and germination

2023

ANRES

View full text Add to dashboard Cite

show abstract

“…Chitosan, a natural biopolymer found in shrimp or insect shells (Malerba and Cerana 2015 ) is a low-cost, safe, and nontoxic compound (Chayjarung et al 2021 ) that has the potential to induce the production of secondary metabolites in plants (Jiao et al 2018 ). Chitosan is known to potentially trigger plant defense responses, forming a semipermeable film around plant tissue (Ghauth et al 1994 ; Pérez-Alonso et al 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Improving galegine production in transformed hairy roots of Galega officinalis L. via elicitation

et al. 2022

View full text Add to dashboard Cite

Galega officinalis L. is an herbaceous legume used to treat symptoms associated with hyperglycemia or diabetes mellitus because of its dominant alkaloid, galegine. In this study, we induced hairy roots in this plant using Rhizobium rhizogenes strain A4, and investigated the effect of type, concentration, and duration of elicitor application on galegine content and some phytochemical characteristics in the hairy roots. Hence, the best growing hairy root line in terms of growth rate was selected and subcultured for treatment with elicitors. Then, at the end of the log phase of growth, chitosan (100, 200, and 400 mg/L), salicylic acid (100, 200, and 300 mM), and ultrasound (1, 2, and 4 min) were applied to hairy roots culture medium. High-performance liquid chromatography (HPLC) showed that the content of galegine was significantly increased after elicitation compared with the control. Thus, the highest content of galegine (14.55 mg/g FW) was obtained 2 days after elicitation when ultrasonic waves were applied to the hairy root culture medium for 4 min. Also, elicitation resulted in a significant increase in the content of total phenol, flavonoid, H2O2 and MDA compared with the control. So that the highest total flavonoid content was obtained in hairy roots that were treated with ultrasonic waves for 4 min and harvested 2 days after elicitation; while, application of 400 mg/L chitosan for 4 days resulted in the highest total phenol (16.84 mg/g FW). Graphical Abstract

show abstract

“…Similarly, treatment of hairy roots from peanut cv. Kalasin 2 with chitosan, MeJA, and CD induced a large amount of arachidin-1 and arachidin-3 [19]. However, a study comparing the biological properties of stilbenoid-rich extracts from hairy roots of different peanut cultivars have not been done thoroughly.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)

2021

View full text Add to dashboard Cite

Peanut produces prenylated stilbenoids upon biotic stress. However, the role of these compounds against oxidative stress have not been thoroughly elucidated. To this end, the antioxidant capacity of extracts enriched in prenylated stilbenoids and derivatives was studied. To produce these extracts, hairy root cultures of peanut cultivars Hull, Tifrunner, and Georgia Green were co-treated with methyl jasmonate, cyclodextrin, hydrogen peroxide, and magnesium chloride and then the stilbenoids were extracted from the culture medium. Among the three cultivars, higher levels of the stilbenoid derivatives arachidin-1 and arachidin-6 were detected in cultivar Tifrunner. Upon reaction with 2,2-diphenyl-1picrylhydrazyl, extracts from cultivar Tifrunner showed the highest antioxidant capacity with an IC50 of 6.004 µg/mL. Furthermore, these extracts had significantly higher antioxidant capacity at 6.25 µg/mL and 3.125 µg/mL when compared to extracts from cultivars Hull and Georgia Green. The stilbenoid-rich extracts from peanut hairy roots show high antioxidant capacity and merit further study as potential nutraceuticals to promote human health.

show abstract

Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Cited by 17 publications

References 39 publications

Improvement of stilbene constituents and nutritional quality of peanut sprouts using abiotic elicitation and germination

Improvement of stilbene constituents and nutritional quality of peanut sprouts using abiotic elicitation and germination

Improving galegine production in transformed hairy roots of Galega officinalis L. via elicitation

Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)

Contact Info

Product

Resources

About