Enhanced characterisation of milk fat globules by their size, shape and refractive index with scanning flow cytometry

Konokhova, Anastasiya I.; Родионов, А. А.; Gilev, Konstantin V.; Mikhaelis, Ilya M.; Strokotov, Dmitry I.; Moskalensky, Alexander E.; Yurkin, Maxim A.; Chernyshev, Andrei V.; Maltsev, Valeri P.

doi:10.1016/j.idairyj.2014.08.006

Cited by 18 publications

(16 citation statements)

References 37 publications

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“…Specifically, light scattering profile (LSP) is measured in a wide range of scattering angles. This approach has been successfully applied to RBCs (26,27), as well as to other biological particles, including platelets, bacteria, blood microparticles, and milk fat globules (24,(28)(29)(30)(31). This approach has been successfully applied to RBCs (26,27), as well as to other biological particles, including platelets, bacteria, blood microparticles, and milk fat globules (24,(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

“…The core of the characterization method is the solution of the inverse light-scattering (ILS) problem, using a parametric shape model of the characterized particle (24,25). This approach has been successfully applied to RBCs (26,27), as well as to other biological particles, including platelets, bacteria, blood microparticles, and milk fat globules (24,(28)(29)(30)(31). Conceptually similar approach, based on LSP measurement combined with fitting theoretical LSPs, has been recently demonstrated for determination of RBC diameters in a microfluidic channel (32).…”

Section: Introductionmentioning

confidence: 99%

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Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

et al. 2017

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Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above-mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle-resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor samples. Our results confirm the possibility of precise, label-free, enhanced morphological analysis of individual intact RBCs with compact single-detector flow cytometer. Detailed characterization of RBCs with high statistics and precision can be used to increase the value of screening examinations and to reveal pathologies accompanied by abnormality of RBC shape. © 2017 International Society for Advancement of Cytometry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

et al. 2017

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“…The new method does not require chemical spherization of RBCs and utilizes the solution of the inverse light-scattering (ILS) problem, using the optical model of a mature RBC. We used similar approach for characterization of lymphocytes, platelets, rod-like bacteria, blood microparticles, and milk fat globules [11][12][13][14]. Extension of this approach to the RBCs requires knowledge of their precise shape.…”

Section: Introductionmentioning

confidence: 99%

Mature red blood cells: from optical model to inverse light-scattering problem

Gilev¹,

Yurkin²,

Chernyshova³

et al. 2016

Biomed. Opt. Express

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Abstract:We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content. ©2016 Optical Society of America

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“…Among a multitude of peak functions we have chosen the beta density because it vanishes at the boundaries of the shape index range, which agrees with natural physical constraints. Second, we set d max 5 1 for fully activated platelets, since we did not find any spherical platelets when applied the algorithm for separation of spherical and spheroidal particles from LSPs (33). So we additionally postulated a number of reasonable constraints.…”

Section: Shape Index Distribution Analysismentioning

confidence: 99%

“…However, setting d min 5 0 also works fine and has only minor effect on the final results (data not shown). Second, we set d max 5 1 for fully activated platelets, since we did not find any spherical platelets when applied the algorithm for separation of spherical and spheroidal particles from LSPs (33). Third, we assumed that distributions of both resting and fully activated platelets are symmetric, since our data gives no reliable indications against it.…”

Section: Shape Index Distribution Analysismentioning

confidence: 99%

Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets

et al. 2016

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Whereas commercially available hematological analyzers measure volume of individual platelets, angle-resolved light-scattering provides unique ability to additionally measure their shape index. We utilized the scanning flow cytometer to measure light-scattering profiles (LSPs) of individual platelets taken from 16 healthy donors and the solution of the inverse light-scattering problem to retrieve the volume and shape index of each platelet. In normal conditions, the platelet shape index distribution (PSID) demonstrates three peaks, which relate to resting, partially activated, and fully activated platelets. We developed an algorithm, based on fitting PSID by a sum of three peak functions, to determine the percentage, mean platelet shape index, and distribution width of each platelet fraction. In total, this method gives eight additional parameters of platelet morphology and function to be used in clinical hematological analysis. We also stimulated the platelets with adenosine diphosphate (ADP) and measured the dependence of equilibrium PSID, including the total percentage of activated platelets, on ADP concentration. © 2016 International Society for Advancement of Cytometry.

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Enhanced characterisation of milk fat globules by their size, shape and refractive index with scanning flow cytometry

Cited by 18 publications

References 37 publications

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

Mature red blood cells: from optical model to inverse light-scattering problem

Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets

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