Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

Gilev, Konstantin V.; Yastrebova, Ekaterina S.; Strokotov, Dmitry I.; Yurkin, Maxim A.; Karmadonova, N.A.; Chernyshev, Andrei V.; Lomivorotov, Vladimir V.; Maltsev, Valeri P.

doi:10.1002/cyto.a.23141

Cited by 28 publications

(25 citation statements)

References 35 publications

(70 reference statements)

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“…Originally, the dataset‐based method was applied to blood platelets (oblate spheroids), [ 48,218 ] E. coli bacteria (capsules), [ 64 ] and microparticle aggregates (bispheres) [ 214 ] with

p = 4

, as well as for RBCs (biconcave discoids) with

p = 5

. [ 91,219 ] In the case of bacteria the confidence region of retrieved characteristics was used for quality control. [ 64 ] For part of the cells this region consisted of two distant domains in coordinates of the length versus the diameter or orientation angle, hence, the region width was compared against a threshold to discard a part of the sample.…”

Section: Characterization Methods and Inverse Problemsmentioning

confidence: 99%

“…The SFC allows one to obtain the scattering intensity, averaged over the azimuthal angle φ, for values of the polar angle θ from 10° to 70° [ 90 ] at a throughput of up to 200 particles per second. [ 91 ] Later the SFC was improved by measuring polarized LSPs to detect particle non‐sphericity [ 92 ] and by simultaneous measurements of forward and side scattering for extremely precise characterization of homogeneous spheres. [ 93 ]…”

Section: Available Techniquesmentioning

confidence: 99%

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Single‐Particle Characterization by Elastic Light Scattering

Romanov

Yurkin

2021

Laser & Photonics Reviews

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The field of light‐scattering characterization of single particles has seen a rapid growth over the last 30 years largely due to the progress in measurement and simulation capabilities. In particular, several methods have been developed to reliably characterize various particles, described by a model with several characteristics, with geometric resolution significantly better than the diffraction limit. However, their development has been largely fragmentary, limited to specific experimental set‐ups. To fill this gap, these lines of development are reviewed within a unified framework. While focusing on characterization algorithms themselves, the experimental aspects related to the isolation and measurement of single particles are also discussed. The existing characterization methods are divided into three classes. The widest class is that of model‐driven methods based on solving parametric inverse light‐scattering problems, using a direct inversion of a low‐dimensional mapping, a nonlinear regression, or neural networks. Other classes include model‐free reconstruction methods and data‐driven classification methods. This review is designed to be extensive in including all relevant literature, but the discussion of semi‐quantitative imaging methods, such as tomography or holography‐based reconstruction, is deliberately omitted. Throughout the review the development of various characterization methods is described, they are critically compared, and promising directions of future research are highlighted.

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“…Originally, the dataset‐based method was applied to blood platelets (oblate spheroids), [ 48,218 ] E. coli bacteria (capsules), [ 64 ] and microparticle aggregates (bispheres) [ 214 ] with

p = 4

, as well as for RBCs (biconcave discoids) with

p = 5

Section: Characterization Methods and Inverse Problemsmentioning

confidence: 99%

Section: Available Techniquesmentioning

confidence: 99%

Single‐Particle Characterization by Elastic Light Scattering

Romanov

Yurkin

2021

Laser & Photonics Reviews

View full text Add to dashboard Cite

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“…Comprehensive details of the scanning flow cytometry approach can be found elsewhere 24 . Particles with arbitrary shapes (rods, spheroids, disks), sizes (from fractions of um to tens of um) and refractive indices (from 1.36 to 1.7) were intensively investigated including bacteria differentiation, 25 platelets activation, 26 and red blood cells analysis 27 …”

Section: Microsphere‐based Methods Of Immunofluorescence Analysismentioning

confidence: 99%

Spectrally encoded microspheres for immunofluorescence analysis

Sankova

Shalaev

Semeykina

et al. 2020

J of Applied Polymer Sci

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A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis. K E Y W O R D S bioengineering, biomedical applications, functionalization of polymers, synthesis and processing techniques 1 | INTRODUCTION Bead-based immunofluorescence analysis is a powerful tool widely used in numerous applications for life science research (extracellular vesicles [EV], 1 cytokines, 2 chemokines, 3 growth factors, cells and drug research, 4 etc.) and for in vitro diagnostics 5 of infectious diseases, diabetes, cardiovascular diseases, inflammation, cancer, 6

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“…We used a scanning flow cytometer (SFC), described in detail elsewhere (37)(38)(39)(40)(41). The SFC was fabricated by Cytonova LLC (Novosibirsk, Russia).…”

Section: Instrumentationmentioning

confidence: 99%

Proposed Dynamics of CDB3 Activation in Human Erythrocytes by Nifedipine Studied with Scanning Flow Cytometry

et al. 2019

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Nifedipine is calcium channels and pumps blocker widely used in medicine. However, mechanisms of nifedipine action in blood are not clear. In particular, the influence of nifedipine on erythrocytes is far from completely understood. In this work, applying scanning flow cytometry, we observed experimentally for the first time the dynamics behind a significant increase of HCO 3 − /Cl − transmembrane exchange rate of CDB3 (main anion exchanger, AE1, Band 3, SLC4A1) of human erythrocytes in the presence of nifedipine in blood. It was found that the rate of CDB3 activation is not limited by the rate of nifedipine binding and/or Ca 2+ transport. In order to explain the experimental data, we suggested a kinetic model assuming that the rate of CDB3 activation is limited by the dynamics of the balance between two intracellular processes (1) the activation of CDB3 limited by its interaction with intracellular Ca 2+ , and (2) the spontaneous deactivation of CDB3. Thus the use of scanning flow cytometry allowed to clarify quantitatively the molecular kinetic mechanism of nifedipine action on human erythrocytes. In particular, the efficiency (~30) and rates of activation (~0.3 min −1 ) and deactivation (~10 −3 min −1 ) of CDB3 in human erythrocytes was evaluated for two donors.Nifedipine is widely used in medicine under the name of Adalat and other drugs. One common usage is the treatment of cardiovascular pathology such as atherosclerosis, coronary heart disease, hypertension, cerebral ischemia, and others (1). Other uses include painful spasms of the esophagus (2,3), Raynaud's phenomenon (4), tocolytic therapy (5). However, the effect of the treatment by nifedipine is not always positive (6). In particular, it is not clear (7) the influence of nifedipine on the rate of HCO 3− /Cl − transmembrane CDB3 protein (AE1, Band 3), which plays an important role in bicarbonate metabolism (8). Overall, a more complete understanding of nifedipine action in blood is needed.Nifedipine belongs to the subclass of dihydropyridines (DHPs), which are known as inhibitors of Ca 2+ transport across the erythrocyte membrane in both pathways: (1) inward leak through Ca 2+ entry channels (9-11), and (2) extrusion by plasma membrane Ca 2+ pumps (PMCA) (12,13). It was reported (13) that about 50% inhibition of PMCA was obtained at extracellular nifedipine concentration of 300 μM. Interestingly, the same extracellular concentration of 300 μM nifedipine was reported (14) to cause less (about 30%) inhibition of Ca 2+ entry channels of erythrocyte membrane. Stronger inhibition of PMCA than entry channels leads to the accumulation of intracellular Ca 2+ in erythrocytes in the presence of extracellular

show abstract

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

Cited by 28 publications

References 35 publications

Single‐Particle Characterization by Elastic Light Scattering

Single‐Particle Characterization by Elastic Light Scattering

Spectrally encoded microspheres for immunofluorescence analysis

Proposed Dynamics of CDB3 Activation in Human Erythrocytes by Nifedipine Studied with Scanning Flow Cytometry

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