Mature red blood cells: from optical model to inverse light-scattering problem

Abstract

Abstract:We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content. ©2016 Optical Society of America

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

“…1c). The diameter, maximal and minimal thicknesses form the primary morphological set, from which other morphological values, namely V, S, SI, spontaneous curvature c 0 , can be calculated, see (27) for a detailed procedure. For example, the diameter of the RBC shown in Fig.…”

“…1b) and solution of the ILS problem as described in our recent article (27). 1b) and solution of the ILS problem as described in our recent article (27).…”

“…Specifically, light scattering profile (LSP) is measured in a wide range of scattering angles. This approach has been successfully applied to RBCs (26,27), as well as to other biological particles, including platelets, bacteria, blood microparticles, and milk fat globules (24,(28)(29)(30)(31). This approach has been successfully applied to RBCs (26,27), as well as to other biological particles, including platelets, bacteria, blood microparticles, and milk fat globules (24,(28)(29)(30)(31).…”

Resolving power of diffraction imaging with an objective: a numerical study

“…Elastic light scattering is modeled by a method of discrete dipole approximation (DDA) using a plane wavefield for the incident beam. We chose an open-source software ADDA which imports n s (r, λ) in a host medium of n h to obtain the Mueller matrix of elements S ij (θ s , φ s ) for calculation of scattered light intensities of different polarization attributes [4,17], where i or j=1, 2, 3 4 and θ s and φ s are respectively the polar and azimuthal angles of scattered light "rays". For simplicity, we limit analysis here to calculation of unpolarized DIs by S 11 (θ s , φ s ).…”

“…Angle-resolved study of elastically scattered light in far-field led to various tools for assay of micrometer-sized particles including biological cells by the contrast mechanism based on the 3D heterogeneity in refractive index (RI) [1][2][3][4]. In comparison, imaging of coherent light scatter has been much less explored for the challenges to acquire and assess high-contrast images [5][6][7][8].…”