Enhanced biocatalysis mechanism under microwave irradiation in isoquercitrin production revealed by circular dichroism and surface plasmon resonance spectroscopy

Gong, An; Zhu, Dan; Mei, Yi-Yuan; Xu, Xiaosen; Wu, Fu‐An; Wang, Jun

doi:10.1016/j.biortech.2016.01.009

Cited by 9 publications

(11 citation statements)

References 34 publications

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“…A value of 2.53 g g −1 was obtained by using grooved immobilized enzymes microchannel reactor. Comparing with the isoquercitrin production per unit of enzyme, the value was increased to 2.75‐fold and 2.77‐fold while the values were 0.920 and 0.915 g g −1 , respectively . The results indicated that more efficient enzyme utilization occurs in grooved immobilized enzymes microchannel reactors with higher yields, even though a small amount of enzyme activity is lost because the active site of the enzyme is affected by immobilization .…”

Section: Resultsmentioning

confidence: 99%

Enzyme immobilized on the surface geometry pattern of groove‐typed microchannel reactor enhances continuous flow catalysis

Zhu

Gong

Zhang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Enzymatic catalysis in a microchannel under continuous flow has attracted increasing scientific interest. It allows for surfaces with higher specificities for heat and mass transfer and makes it easier to control reaction parameters. However, its applications are limited by a lesser amount of loading and cycle of use of the immobilized enzyme. A series of groove-typed channel microreactors with surface geometry patterns were first established for effective immobilization of naringinase, which was used to produce isoquercitrin from rutin via hydrolysis. RESULTS:Of the tested groove-typed channel microreactors with surface geometry patterns, the enzyme adsorbing capacities of all were increased compared to that with smooth surface. The enzyme adsorbing capacity increased 1.32-fold in the grooved microreactor with triangular pattern. Excellent yields of isoquercitrin production were produced in grooved channel immobilized enzyme microreactors with triangular surfaces. A yield of 91.72 ± 0.65% was obtained in 10 min, the microreactor could be reused 12 times with a residual activity over 56%. Efficient transfer of heat and mass were attained in the grooved microreactors with surface geometry patterns. The Poiseuille number and Nusselt number were increased by 20.61% and 29.63%, respectively, using fluid mechanics analysis in the grooved microreactor with triangular pattern. CONCLUSION: The grooved microreactor with geometry pattern surface provided an efficient method for the high production of isoquercitrin, which is promising for enzyme immobilization and catalysis.

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Section: Resultsmentioning

confidence: 99%

Enzyme immobilized on the surface geometry pattern of groove‐typed microchannel reactor enhances continuous flow catalysis

Zhu

Gong

Zhang

et al. 2019

J of Chemical Tech & Biotech

Self Cite

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“…Whole cell catalysts typically caused flocculation, which results in a decrease in its mass transfer and catalytic efficiency. In addition, some efforts have been made in the study of the crude enzyme or the recombinant enzyme . However, the activity of the crude enzyme was unstable, and obtaining the recombinant enzyme was complicated and had a high purification cost.…”

Section: Introductionmentioning

confidence: 94%

“…α ‐L‐rhamnosidases have been used as free enzymes, immobilized enzymes and whole cell catalyst forms . Due to the diversity of natural α ‐L‐rhamnosidase sources, the complexity of the multicomponents and the difficulties in separation, the price of this enzyme is extremely high, resulting in its limited application . The catalytic efficiency of the immobilized enzyme was reduced due to the presence of the immobilized site.…”

Section: Introductionmentioning

confidence: 99%

“…10 Due to the diversity of natural -L-rhamnosidase sources, the complexity of the multicomponents and the difficulties in separation, the price of this enzyme is extremely high, resulting in its limited application. 11 The catalytic efficiency of the immobilized enzyme was reduced due to the presence of the immobilized site. Whole cell catalysts typically caused flocculation, which results in a decrease in its mass transfer and catalytic efficiency.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, some efforts have been made in the study of the crude enzyme or the recombinant enzyme. 11 However, the activity of the crude enzyme was unstable, and obtaining the recombinant enzyme was complicated and had a high purification cost. Thus, important challenges remain for the application of the -L-rhamnosidase in a microreactor.…”

Section: Introductionmentioning

confidence: 99%

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The combined use of a continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient biocatalysis of unpurified recombinant enzyme

Wang

Zhu

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Microfluidic biocatalysis has advantages in terms of heat transfer and mass transfer. However, mass transfer in microscale conditions is often limited by excessive enzyme agglomeration, resulting in reduced reactor performance. In the present study, to establish an efficient and inexpensive microreaction system for obtaining high‐value fine chemicals, a novel combined use of continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient production of isoquercitrin from rutin catalysed by unpurified recombinant enzyme RhaB1 was successfully carried out. RESULTS The unpurified RhaB1 was expressed by BL21‐pET21a‐rhaB1 and the amino acid sequence was confirmed by Maldi‐TOF‐TOF, which was directly applied in microchannel reactors with an increased activity of 37.7% compared with the purified RhaB1, with [Toma][Tf2N] used as a cosolvent to avoid enzymatic agglomeration. A yield of 99.3 ± 4.3% was achieved in 10 min using crude RhaB1 in microchannel reactors under optimum conditions, and circular dichroism spectroscopy confirmed that [Toma][Tf2N] had a positive effect on RhaB1 activity. Compared with a batch reactor, the reaction time was reduced by 98.3%, the Km was decreased to 1/3 and productivity was improved 61.4 times. CONCLUSION Results indicated that the developed cosolvent system in the microfluidic reactor could improve the solubility and liquidity of recombinant RhaB1. The protocol is promising for industrial applications due to higher productivity and lower costs. © 2018 Society of Chemical Industry

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Flavonoid Glycoside Transformation Catalyzed by Whole-Cell Catalysts Using a PVDF Membrane Reactor Coupled with Reaction and Separation

Huang

Zhang

Wang

et al. 2020

Waste Biomass Valor

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Enhanced biocatalysis mechanism under microwave irradiation in isoquercitrin production revealed by circular dichroism and surface plasmon resonance spectroscopy

Cited by 9 publications

References 34 publications

Enzyme immobilized on the surface geometry pattern of groove‐typed microchannel reactor enhances continuous flow catalysis

Enzyme immobilized on the surface geometry pattern of groove‐typed microchannel reactor enhances continuous flow catalysis

The combined use of a continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient biocatalysis of unpurified recombinant enzyme

Flavonoid Glycoside Transformation Catalyzed by Whole-Cell Catalysts Using a PVDF Membrane Reactor Coupled with Reaction and Separation

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