“… 10 In addition, whole-cell biocatalysts are commonly used when cofactors are involved in the reaction, 14 such as the reactions catalyzed by oxidoreductases that depend on nicotinamide cofactors 15,16 and the redox-neutral multi-step reactions coupling of enzymes. 17,18 …”
Fermentation process was applied to relieve the substrate transport-limitation of P. pastoris whole-cell biocatalysts, which was much simpler, more energy-saving and greener than c traditional permeabilizing reagent and ultrasonication treatment.
“… 10 In addition, whole-cell biocatalysts are commonly used when cofactors are involved in the reaction, 14 such as the reactions catalyzed by oxidoreductases that depend on nicotinamide cofactors 15,16 and the redox-neutral multi-step reactions coupling of enzymes. 17,18 …”
Fermentation process was applied to relieve the substrate transport-limitation of P. pastoris whole-cell biocatalysts, which was much simpler, more energy-saving and greener than c traditional permeabilizing reagent and ultrasonication treatment.
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