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Fermentation process was applied to relieve the substrate transport-limitation of P. pastoris whole-cell biocatalysts, which was much simpler, more energy-saving and greener than c traditional permeabilizing reagent and ultrasonication treatment.

A kind of UV-curable cyclohexanone-formaldehyde resins (UVCF) was prepared by the reaction of cyclohexanone-formaldehyde resin (CFR), isophorone diisocyanate (IPDI) and 2-hydroxyethyl acrylate (HEA). The UVCFIPID-HEA obtained was characterized by fourier transform infrared spectrum (FTIR), nuclear magnetic resonance (NMR). The FTIR and 1H-NMR results show that the terminal acrylate acyloxy was introduced into the structure of the prepolymer. The results of solvent resistance tests show that the solvent resistance property of UVCFIPID-HEA is excellent.