Effective synthesis of Rebaudioside A by whole-cell biocatalyst Pichia pastoris

Chen, Meiqi; Xin, Zeng; Zhu, Qingjuan; Wang, Denggang; Han, Sang‐Ik; Liang, Shuli; Lin, Ying

doi:10.1016/j.bej.2021.108117

Cited by 11 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A higher application temperature in biocatalyst production ensures reduction in microbial contamination, better solubility, and often a more favorable equilibrium position ( Bommarius and Paye, 2013 ). In a previous study, the optimal temperature for Reb A synthesis by the whole-cell biocatalyst was 50°C, which was favorable for industrial application ( Chen et al, 2021 ). Considering the possibility of combining PgUGT and UGT76G1 to utilize St as a substrate for Reb D synthesis, the thermostability of PgUGT should be improved.…”

Section: Resultsmentioning

confidence: 99%

“…The price of SG components is directly related to the nature and difficulty of extraction. Presently, with St as the substrate, a high yield of valuable Reb A was efficiently synthesized via biocatalysts ( Chen et al, 2021 ). Among the SGs, rebaudioside D (Reb D) is one of the most important components, which has a much better flavor and higher sweetness than most other SGs, including Reb A and St ( Kovacevic et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis

Chen

Song

Qin

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

As a natural sweetener and sucrose substitute, the biosynthesis and application of steviol glycosides containing the component rebaudioside D have attracted worldwide attention. Here, a glycosyltransferase PgUGT from Panax ginseng was first reported for the biosynthesis of rebaudioside D. With the three-dimensional structures built by homology modeling and deep-learning–based modeling, PgUGT was semi-rationally designed by FireProt. After detecting 16 site-directed variants, eight of them were combined in a mutant Mut8 with both improved enzyme activity and thermostability. The enzyme activity of Mut8 was 3.2-fold higher than that of the wild type, with an increased optimum reaction temperature from 35 to 40°C. The activity of this mutant remained over 93% when incubated at 35°C for 2 h, which was 2.42 times higher than that of the wild type. Meanwhile, when the enzymes were incubated at 40°C, where the wild type was completely inactivated after 1 h, the residual activity of Mut8 retained 59.0% after 2 h. This study would provide a novel glycosyltransferase with great potential for the industrial production of rebaudioside D and other steviol glycosides.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis

Chen

Song

Qin

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The stevioside conversion percentage was 62.5%, which is quite remarkable given that traditional methods of bioconversion were applied to produce rebaudioside A [9]. With the help of UDP-glycosyltransferase 76G1 and the co-expression of sucrose synthase in Pichia pastoris GS115, Chen et al reached a 88.9% stevioside conversion to produce 261.2 mM rebadudioside A in a newly implemented feeding cascade bioconversion [10]. Both conventional and microwave-assisted bioconversions have advantages and disadvantages.…”

Section: Practical Applicationmentioning

confidence: 99%

“…With the help of UDP‐glycosyltransferase 76G1 and the co‐expression of sucrose synthase in Pichia pastoris GS115, Chen et al. reached a 88.9% stevioside conversion to produce 261.2 mM rebadudioside A in a newly implemented feeding cascade bioconversion [10]. Both conventional and microwave‐assisted bioconversions have advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13

Czinkóczky

Németh

2021

Engineering in Life Sciences

View full text Add to dashboard Cite

Stevia rebaudiana is a sweet herbaceous perennial plant, which is frequently used in the preparation of plant-based sweeteners. The demand for such sweeteners continues to increase due to purposeful nutrition and modern-day metabolic syndromes. More than 20 types of steviol glycosides provide a sweet taste, which are more than 300 times sweeter than sucrose. They are formed of two main components, namely stevioside and rebaudioside A. Only a handful of studies have dealt with Stevia rebaudiana leaf extracts, the conversion of pure stevioside into the preferred rebaudioside A is more common. The aim of this study was to enrich the rebaudioside A content of Stevia rebaudiana leaf extract using enzymatic bioconversion by applying fermented cyclodextrin glycosyltransferase from Bacillus licheniformis DSM13. Two differently processed plant materials, namely dried and lyophilized Stevia rebaudiana plants, were extracted and compared. Following the bioconversion, the rebaudioside A content was on average doubled. The maximum increase was fivefold with a 70-80% conversion of the stevioside.

show abstract

“…The price of SG components is directly related to the nature and difficulty of extraction. Presently, with St as the substrate, a high yield of valuable Reb A was efficiently synthesized via biocatalysts (Chen et al, 2021). Among the SGs, rebaudioside D (Reb D) is one of the most important components, which has a much better flavor and higher sweetness than most other SGs, including Reb A and St (Kovacevic et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Image1.tif

View full text Add to dashboard Cite

Effective synthesis of Rebaudioside A by whole-cell biocatalyst Pichia pastoris

Cited by 11 publications

References 28 publications

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis

Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13

Image1.tif

Contact Info

Product

Resources

About