Enzyme immobilized on the surface geometry pattern of groove‐typed microchannel reactor enhances continuous flow catalysis

Abstract

BACKGROUND: Enzymatic catalysis in a microchannel under continuous flow has attracted increasing scientific interest. It allows for surfaces with higher specificities for heat and mass transfer and makes it easier to control reaction parameters. However, its applications are limited by a lesser amount of loading and cycle of use of the immobilized enzyme. A series of groove-typed channel microreactors with surface geometry patterns were first established for effective immobilization of naringinase, which was used to produce isoquercitrin from rutin via hydrolysis. RESULTS:Of the tested groove-typed channel microreactors with surface geometry patterns, the enzyme adsorbing capacities of all were increased compared to that with smooth surface. The enzyme adsorbing capacity increased 1.32-fold in the grooved microreactor with triangular pattern. Excellent yields of isoquercitrin production were produced in grooved channel immobilized enzyme microreactors with triangular surfaces. A yield of 91.72 ± 0.65% was obtained in 10 min, the microreactor could be reused 12 times with a residual activity over 56%. Efficient transfer of heat and mass were attained in the grooved microreactors with surface geometry patterns. The Poiseuille number and Nusselt number were increased by 20.61% and 29.63%, respectively, using fluid mechanics analysis in the grooved microreactor with triangular pattern. CONCLUSION: The grooved microreactor with geometry pattern surface provided an efficient method for the high production of isoquercitrin, which is promising for enzyme immobilization and catalysis.

Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products

“…Results showed that the microreactor enhanced up to 10 times the system productivity compared to the free enzyme form and 30 times compared to the batch immobilized system. The superior characteristics of microreactors in terms of productivity, reaction time, and amount of biocatalysts used in comparison with other types of microreactors are in accordance with the observations made by previous studies by Tušek et al and Zhu et al for surface immobilized enzymes in different microreactor systems. , …”

“…The superior characteristics of microreactors in terms of productivity, reaction time, and amount of biocatalysts used in comparison with other types of microreactors are in accordance with the observations made by previous studies by Tusěk et al and Zhu et al for surface immobilized enzymes in different microreactor systems. 35,36 Morphological and Optical Characterization of the Developed Microbioreactor. As a proof of concept, the nanostructure developed in the inner walls of the glass capillaries was characterized with electron microscopy and spectroscopic techniques to investigate the morphology of ZnO nanostructures and confirm the effective PEI functionalization.…”

Characterization and evaluation of immobilized enzymes for applications in flow reactors

“…printed reactors [32] and groove-typed channel microreactors have been tailored to increase the loading capacity [33]. In wall-coated reactors the STY scales reciprocally with the diameter of the channel.…”

Heterogeneous Microfluidic Reactors: A Review and an Insight of Enzymatic Reactions

“…The effect of surface geometry of the microreactor on the immobilization of biocatalysts was also an important parameter. Zhu et al studied the impact of different geometry patterns of groove-typed channel microreactors on the immobilization of naringinase and further investigated the catalytic performance through hydrolysis of rutin-producing isoquercitrin [249]. The groove-type microreactors increased the enzyme adsorbing capacities compared to the smooth surface, giving the maximum of 1.32-fold increment in a triangular pattern.…”