Enhance of IL-22 expression in Oral Candidiasis Immunosupressed Model with <i>Acanthus ilicifolius</i> Extract Therapy

Andriani, Dwi; Pargaputri, Agni Febrina

doi:10.1088/1755-1315/217/1/012056

Cited by 5 publications

(12 citation statements)

References 19 publications

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“…12,22 Previous studies of both nystatin and A. ilicifollius extract (8% and 16%) found that the latter were similar to nystatin in increasing the amount of IL-17 expression in oral candidiasis immunosuppressed conditions models. 18 This indicates that the capture of TLR-2 leads to a decrease in the number of candida and increased pro-inflammatory cytokines. In this study, the concentration of 8% A. ilicifolius extract produced the effect on the increase of proinflammatory cytokines via TLR-2 pathway.…”

Section: Discussionmentioning

confidence: 99%

“…The administration of chloroform extract A. ilicifolius 8 mg/ml may increase IL-17 in immunosuppressed subjects suffering from oral candidiasis. 17,18 The purpose of this study was to compare the effect of A. ilicifolius chloroform extract and nystatin on TLR-2 expression immunosuppressed models as an alternative medicine derived from natural materials for oral candidiasis.…”

Section: Introductionmentioning

confidence: 99%

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The effects of Acanthus ilicifolius chloroform extract on TLR-2 expression of macrophages in oral candidiasis

Andriani

Pargaputri

2018

Dent. J.

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Background: Immunosuppressed conditions due to long-term corticosteroid and tetracycline consumption are susceptible to fungal invasion, especially by Candida albicans (C. albicans), that requires treatment of oral candidiasis. Toll like receptor-2 (TLR-2) plays a role in candida recognition. Nystatin is regularly employed for oral candidiasis, but produces certain side-effects. Chloroform extract of Acanthus ilicifolius (A. ilicifolius) leaves represents both a potent inhibitor of C. albicans growth and an antioxidant. Purpose: This study aimed to compare the effect of A. ilicifolius leaf chloroform extract and nystatin treatment on TLR-2 expression in oral candidiasis immunosupressed models. Methods: This study constitutes a true experimental investigation incorporating a post test-only control group design. 20 healthy male Rattus novergicus (Wistar), aged 12 weeks and with an average weight of 250g, were immunosuppressed through oral administration of dexamethasoneand tetracycline for a period of 21 days before being induced with C. albicans (ATCC-10231) 6 x 108 for two weeks. The subjects were divided into five groups (n=4/group): healthy (H), no-treatment(P), nystatin treatment(N), A. Ilicifollius (8%) treatment (AI-1) and A. ilicifollius (16%) treatment (AI-2). The subjects were treated for 14 days, with their tongue being subsequently biopsied. TLR-2 expression was subjected to immunohistochemical examination, observed under a microscope (400x magnification) and statistically analyzed (one-way Anova, LSD-test, p<0.05). Results: TLR-2 expression of P (6.25 ± 2.5), N (11.25 ± 0.96), AI-1 (13.00 ± 1.15), AI-2 (12.75 ± 1.7) was higher than H (1.75 ± 0.5). Significant differences existed between N to P, N, AI-1, AI-2; P to N, AI-1 and AI-2 (p<0.05). No significant differences were present between N, AI-1 and AI-2 (p < 0.05). Conclusion: A. ilicifolius extract can increase expression of TLR-2 in oral Candidiasis-immunosuppressed models. A. ilicifolius extract produces the same effect in increasing TLR-2 expression when compared to nystatin.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effects of Acanthus ilicifolius chloroform extract on TLR-2 expression of macrophages in oral candidiasis

Andriani

Pargaputri

2018

Dent. J.

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“…It is already known that Acanthus ilicifolius extract has antifungal and antioxidant in-vitro and became antifungal to mice that suffered from oral candidiasis 19,23,24 . This study confirmed that the immerse time in effervescent Acanthus ilicifolius extract 4% (Fig.…”

Section: Discussionmentioning

confidence: 99%

The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic

Prananingrum

Rahmitasari

Andriani

et al. 2022

Odonto : Dental Journal

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Background: Acanthus ilicifolius L. has antifungal potential and makes it possible to be a candidate for denture cleanser. This study aims to evaluate the effect of effervescent Acanthus ilicifolius L. extract on antifungal properties and flexural strength acrylic after immersion for three duration times.Method: Extracts were made by maceration method with ethanol. For the antifungal test, effervescent Acanthus ilicifolius L extract was prepared by combining the Acanthus ilicifolius L extract 4% and sodium perborate with two ratios, 1:7 and 1:14. The microdilution method was carried out. Effervescent Acanthus ilicifolius L extract 4% with ratio 1:7 was prepared for the flexural strength test. The fifty-four heat-cured acrylic resin (20x10x2.5mm) were fabricated and divided into 3 groups. Group 1 was immersed in aquadest (control); Group 2 was immersed in sodium perborate; Group 3 was immersed in effervescent Acanthous ilicifolius L. extract. Each group was immersed for 15 min, 8, and 56 hours (n=6). The flexural strength test was performed using Universal Testing Machine.Result: Effervescent Acanthus ilicifolius L. extract exhibited antifungal activity which increased in an effervescent Acanthus ilicifolius L extract-dependent manner significantly (0.57 to 0.50 OD). There were no significant differences between sodium perborate, effervescent_AI1 (1:7) and effervescent_AI2 (1:14). The flexural strength was found to be within the range of 66.1 - 68.3 MPa. There were no significant differences between groups (p < 0.05).Conclusion: Effervescent Acanthus ilicifolius L extract with the ratios 1:7 had a good antifungal effect and appropriate flexural strength after immersion for 15 min, 8, and 56 hours.

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“…On the 4 th day, we reduce the dose as much as 10% for dexamethasone and tetracycline, then rats were induced with Candida albicans (ATCC-10231) 6x10 8 as much as 0,1 cc, applied on the dorsum tongue of rats using a sterile cotton bud, given once every two days for 12 days. 11,13 Hyperbaric oxygen therapy was given to the G3 group for five days. During therapy with hyperbaric oxygen, rats were still given tetracycline 0,1 mg/day to prevent bacterial infection.…”

Section: Methodsmentioning

confidence: 99%

Serum Albumin Levels of Oral Candidiasis Immunosuppressed Rats Treated With Hyperbaric Oxygen

Pargaputri¹,

Andriani²

2020

ijihs

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Objective: To investigate serum albumin levels in oral candidiasis immunosuppressed rats treated with hyperbaric oxygen. One of the predisposing factors for oral candidiasis was the use of immunosuppressive drugs continuously. It can also affect the work of the liver because it’s one of the organs responsible for drug metabolism. Hyperbaric oxygen therapy was used not only to suppressing fungal infections, but also to improve liver function by evaluating the serum albumin levels. Methods: This study used a post-test only control group design. Fifteen Wistar rats were divided into 3 groups(n=5/3): G1 (healthy group), G2 (oral candidiasis immunosuppressed rats group without hyperbaric oxygen therapy), and G3 (oral candidiasis immunosuppressed rats group with hyperbaric oxygen therapy). G2 and G3 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetracycline 1 mg/day/rat. Hyperbaric oxygen therapy was given to the G3 group in 5 days. Blood serum of rats in all groups was taken to calculate albumin levels. Results: The average value of albumin levels in G2 group showed a decrease compared to the G1 group, while G3 showed the highest level. One way Anova test showed a significant difference among groups (p<0,05). To compare the difference between each group we used LSD test and showed a significant difference (p<0,05) between G1 compared to G2, G1 compared to G3, and G2 compared to G3. Conclusion: Liver albumin levels of oral candidiasis immunosuppressed rats treated with hyperbaric oxygen therapy showed higher levels than those without therapy.

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Enhance of IL-22 expression in Oral Candidiasis Immunosupressed Model with Acanthus ilicifolius Extract Therapy

Cited by 5 publications

References 19 publications

The effects of Acanthus ilicifolius chloroform extract on TLR-2 expression of macrophages in oral candidiasis

The effects of Acanthus ilicifolius chloroform extract on TLR-2 expression of macrophages in oral candidiasis

The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic

Serum Albumin Levels of Oral Candidiasis Immunosuppressed Rats Treated With Hyperbaric Oxygen

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