Endothelium  in Vivo  and  in Vitro. Part 1: Histogenesis,  Structure, Cytophysiology  and  Key  Markers

Стрельникова, Е А; Trushkina, P.Yu.; Surov, I.Yu.; Korotkova, N.V.; Mzhavanadze, N.D.; Деев, Р. В.

doi:10.23888/hmj201973450-465

Cited by 14 publications

(3 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Одними из наиболее часто используемых линий являются эндотелиальные клетки пупочной вены человека (англ. human umbilical vein endothelial cells, HUVEC) [12,13]. Клеточная линия HUVEC имеет ряд преимуществ: достаточно простые методы выделения и легкое культивирование, а также их относительно низкая стоимость [14].…”

Section: основные клеточные линии используемые для изучения эндотелиunclassified

Endothelium in Vivo and in Vitro. Part 2: Features and Perspectives of Laboratory Work With Endothelial Cells

Mzhavanadze¹,

Korotkova²,

Стрельникова³

et al. 2020

HMJ

Self Cite

View full text Add to dashboard Cite

The second part of the article aims at delivering stateof-the art information of the basics of laboratory work with endothelial cells, including main endothelial cell culture lines for in vitro experiments, 2D and 3D cultivation of endothelial cells. The authors also discuss current issues and perspectives of laboratory research work involving endothelial cells, derived from embryonic stem cells and induced pluripotent stem cells. In vivo study of endothelial cells and assessment of their morphology and function are associated with substantial technical difficulties, which necessitates the use of in vitro techniques aimed at evaluating pathogenesis and potential treatment methods of a number of diseases associated with endothelial dysfunction. Laboratory work involving cell cultures allows studying both physiological and pathological conditions using miniature and often automated systems, which has several considerable benefits over clinical trials and experiments carried out on animals. In vitro techniques have certain limitations, among which are the difficulties in interpreting the results of a work performed on a cell line and comparing those to in vivo conditions. Constant improvement of laboratory methods involving different endothelial cell lines, co-cultures, 3D cultivation systems, and induced pluripotent stem cells, is crucial. Understanding the principles of in vitro work with endothelial cell cultures and optimization of the current techniques will lead to the development and implementation of the accurate laboratory methods, which will help translate research into practice.

show abstract

Section: основные клеточные линии используемые для изучения эндотелиunclassified

Endothelium in Vivo and in Vitro. Part 2: Features and Perspectives of Laboratory Work With Endothelial Cells

Mzhavanadze¹,

Korotkova²,

Стрельникова³

et al. 2020

HMJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…Large groups of population in developed countries all over the world suffer from atherosclerosis of coronary, carotid, or peripheral arteries. Development of atherothrombotic complications remains the main cause of lethal outcomes [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Expression of Apoptotic Markers Bcl-2 and Bax in the Vascular Wall

Klimentova¹,

Suchkov²,

Shchulkin³

et al. 2021

Sovrem Tehnol Med

View full text Add to dashboard Cite

The aim of the study was to assess the levels of Всl-2 and Bax proteins in the vascular wall and their correlation with serum cholesterol in patients with stage III-IV atherosclerosis obliterans of lower limb arteries.Materials and Methods. The study included 32 patients with stage III-IV atherosclerosis obliterans of the lower limb. Samples of intraoperative material (all three layers of the vascular wall) including an atherosclerotic plaque (AP) were taken during primary open surgery on major leg arteries. As a control, we used samples of the arterial wall without visible signs of atherosclerosis. Based on AP ultrasonography, the patients were divided into two groups: with APs of mixed echogenicity and with hyperechoic (calcified) AP. The vascular samples were crushed and homogenized for further measurements of Всl-2 and Bax proteins; in a separate setup, cholesterol in blood serum was measured.Results. In patients without atherosclerotic changes, the level of the anti-apoptotic protein Bcl-2 in the arterial wall was 1.25 ng/mg, and that of the pro-apoptotic protein Bax -4.7 ng/mg. In the case of APs of mixed echogenicity, the expression of Bcl-2 was 1.8 ng/mg (p=0.143) and that of Bax -5.1 ng/mg (p=0.834), with no significant differences from AP-free vascular wall samples. In the arterial wall containing a heterogeneous calcified AP, the expression of Bcl-2 was 0.9 ng/mg (p=0.143), In contrast, the level of Bax was 6.8 ng/mg, which showed its significant increase as compared with the non-AP controls (p=0.02). In the cases with predominantly hyperechoic AP, the expression of Bcl-2 was significantly lower (p=0.036), and that of Bax -significantly higher (p=0.036) in comparison with AP of mixed echogenicity. In patients with hyperechoic AP, we found a negative correlation between the Bax and Bcl-2 values (r=-0.315) and a positive correlation between the Bax expression and serum cholesterol (r=0.617). Conclusion.In arterial walls with hyperechoic (calcified) APs, the expression of anti-apoptotic protein Bcl-2 is reduced, and that of pro-apoptotic protein Bax is increased, which indicates the apoptosis activation in advanced atherosclerotic lesions. In patients with such APs, elevated cholesterol levels directly correlate with the increased expression of pro-apoptotic Bax protein (r=0.617).

show abstract

“…Данная статья посвящена современным аспектам понимания роли эндотелия и его дисфункции в процессах миогенеза и регенерации мышечной ткани в норме и патологических условиях, обусловленных наследственными прогрессирующими заболеваниями мышечной системы -миодистрофиями, в частности, дисферлинопатиями, а также возможности применения эндотелиальных клеток в терапии пациентов с данной патологией. Ключевые слова: мышечная миодистрофия; эндотелиальная дисфункция; дисферлинопатия; миогенез Традиционно нарушение функции эндотелиальных клеток (ЭК) -эндотелиальная дисфункция (ЭД), рассматривается как важный элемент возникновения и прогрессирования различных сосудистых заболеваний, таких как атеросклероз, гипертоническая болезнь, сосудистые осложнения сахарного диабета, а также болезней, где существенную роль играют аутоиммунные и воспалительные процессы, опосредованные эндотелием [1][2][3]. В контексте оценки патогенеза более редких заболеваний ЭД упоминается значительно реже, тем не менее, исследования последних лет проливают свет на участие ЭК и ассоциированных с ними сигнальных путей в развитии тяжелой наследственной патологии, например, миодистрофий.…”

unclassified

Endothelial dysfunction in muscular dystrophies

Каlinin¹,

Suchkov²,

Mzhavanadze³

et al. 2021

HMJ

Self Cite

View full text Add to dashboard Cite

Endotheliocytes are the key elements of the vascular wall and are involved in regulation of vascular tone and permeability, inflammation, hemostasis, angiogenesis etc. Impaired function of endothelial cells universally recognized as endothelial dysfunction is associated with a number of common diseases such as ischemic heart disease, arterial hypertension, atherosclerosis, peripheral arterial disease, septic shock, chronic kidney disease, obesity, oncological and autoimmune diseases. Less is known about the role of endothelial cells in pathogenesis of development and progression of rare diseases, such as muscular dystrophies. Muscular dystrophies involve over 30 genetically determined diseases, which are characterized by the development of a progressive muscular weakness and skeletal muscle degeneration. Presence of a nucleotide variant associated with a certain muscular dystrophy is primarily marked by a limited potential of skeletal muscle regeneration due to the impaired structure and function of myogenic cells. Inherited myopathies include a group of severe neuromuscular diseases caused by a mutation in the dysferlin gene DYSF, which leads to the synthesis of a dysfunctional dysferlin. Complex molecular and cellular interactions involved in skeletal muscle damage and endothelial dysfunction play an important role in the pathogenesis of dysferlinopathies. The possibility to produce an effect on different pathological aspects of dysferlinassociated myopathies such as complement system activation, inflammation, impaired function of endothelial cells, damage and necrosis of myofibrils are extensively studied in vitro and in vivo. This article is dedicated to the current understanding of relationship between the endothelium and its dysfunction in myogenesis and skeletal muscle regeneration in normal and pathological conditions caused by a group of inherited progressive myodystrophies, dysferlinopathies in particular, as well as possible clinical application of endothelial cells in treatment of muscular dystrophies.

show abstract

Endothelium in Vivo and in Vitro. Part 1: Histogenesis, Structure, Cytophysiology and Key Markers

Cited by 14 publications

References 2 publications

Endothelium in Vivo and in Vitro. Part 2: Features and Perspectives of Laboratory Work With Endothelial Cells

Endothelium in Vivo and in Vitro. Part 2: Features and Perspectives of Laboratory Work With Endothelial Cells

Expression of Apoptotic Markers Bcl-2 and Bax in the Vascular Wall

Endothelial dysfunction in muscular dystrophies

Contact Info

Product

Resources

About