Endothelial differentiation of canine yolk sac cells transduced with VEGF

Fratini, Paula; Carreira, Ana Cláudia Oliveira; Alcântara, Dayane; Silva, Fernanda Menezes de Oliveira e; Rodrigues, Márcio Nogueira; Miglino, María Angélica

doi:10.1016/j.rvsc.2015.11.010

Cited by 18 publications

(14 citation statements)

References 32 publications

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“…Total RNA was extracted from amniotic cells using the Trizol manufacturer’s (catalogue number: 12183555 Invitrogen, Carlsbad, CA, USA) protocol, treated with DNAseI (Thermo Scientific), and 1 μg was reverse-transcribed to first-strand cDNA and subjected to quantitative real-time reverse transcription PCR (RT-qPCR) according to Fratini et al [ 39 ]. The primer sequences ( Lin28a , c-myc , GAPDH ) are presented in Table 2 .…”

Section: Methodsmentioning

confidence: 99%

Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells

Borghesi

Mario

Carreira³

et al. 2017

Stem Cell Res Ther

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BackgroundStem cells are capable of unlimited self-renewal and are able to remain undifferentiated for extended periods of time prior to their differentiation into specific cell lineages. Because of the issues (ethical and religious) involved in the use of embryonic stem cells and the limited plasticity of adult stem cells, an alternative cell source could be foetal stem cells derived from extra-embryonic tissue, which are highly proliferative, grow in vitro and possess interesting immunogenic characteristics. As a result, the amniotic membrane of several species has been studied as an important new source of stem cells.MethodsHere, we cultured and characterized mesenchymal progenitor cells derived from the rabbit amniotic membrane, and investigated their differentiation potential. In total, amniotic membranes were collected from eight rabbit foetuses and were isolated by the explant technique. The obtained cells were cultured in DMEM-HIGH glucose and incubated at 37 °C in a humidified atmosphere with 5% CO2.ResultsThe cells adhered to the culture plates and showed a high proliferative capacity with fibroblast-like morphologies. The cells showed a positive response for markers for the cytoskeleton, mesenchymal stem cells and proliferation, pluripotency and haematopoietic precursor stem cells. However, the cells were negative for CD45, a marker of haematopoietic cells. Furthermore, the cells had the capacity to be induced to differentiate into osteogenic, adipogenic and chondrogenic lineages. In addition, when the cells were injected into nude mice, we did not observe the formation of tumours.ConclusionsIn summary, our results demonstrate that multipotent mesenchymal stem cells can be obtained from the rabbit amniotic membrane for possible use in future cell therapy applications.

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Section: Methodsmentioning

confidence: 99%

Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells

Borghesi

Mario

Carreira³

et al. 2017

Stem Cell Res Ther

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“…cDNA was synthesized using 500 ng total RNA, amplification of the resulting cDNAs and analyses were performed as previously described by Fratini and collaborators (Fratini et al 2016). To determine the best endogenous gene, three different genes were tested (GAPDH, RPLP0, Myo7A) (Table 1) and the stability analysis was performed using RefFinder (Xie et al 2012).…”

Section: Flow Cytometrymentioning

confidence: 99%

Cochlear epithelial of dog fetuses: a new source of multipotent stem cells

et al. 2017

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Hearing loss caused by the damage of cochlea sensory cells or neurons is a common human disease, but also affects dogs and other animals. To test their progenitor nature as potential value for future therapies, we characterized cells derived from the cochlear epithelium in dog fetuses. In total, 8 fetuses of 35-40 days of gestation, derived from castration campaigns, were investigated. Cells were analysed by the MTT colorimetric assay and in regard to cell cycle, differentiation capacities, immunophenotypes and qPCR analysis. In culture, cells had a fibroblast-like morphology. Phenotypic immunocharacterization showed positive staining for mesenchymal stem cell and pluripotency markers and were negative for hematopoietic cell markers. Cells possessed differentiation capacity for the three main cell lineages: osteogenic, adipogenic and chondrogenic, altogether indicating their nature as mesenchymal stem cells. Thus, cells derived from fetal cochlear tissues indeed may provide valuable sources of progenitor cells for cell therapy of canine deafness and other diseases.

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“…Canine yolk sac cells transduced with VEGFeGFP (YSVEGF) previously characterized by our group 30 were used to recellularize the canine placenta scaffold. These cells have endothelial progenitor characteristics and capacity to form sproutings in functional assays.…”

Section: Introductionmentioning

confidence: 99%

Canine Placenta Recellularized Using Yolk Sac Cells with Vascular Endothelial Growth Factor

Fratini

Rigoglio

Matias

et al. 2018

BioResearch Open Access

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Regenerative medicine has been growing because of the emergent need for tissues/organs for transplants and restorative surgeries. Biological scaffolds are important tools to try to solve this problem. The one used in this reserach was developed by an acellular biological scaffold from canine placenta with a rich source of cellular matrix. After decellularization, the cellular matrix demonstrated structural preservation with the presence of important functional proteins such as collagen, fibronectin, and laminin. We used cells transduced with vascular endothelial growth factor (VEGF) to recellularize this scaffold. It was succeeded by seeding the cells in nonadherent plaques in the presence of the sterelized placenta scaffold. Cells were adhered to the scaffold when analyzed by immunocytochemistry and scanning electron microscopy, both showing sprouting of yolk sac VEGF (YSVEGF) cells. This recellularized scaffold is a promissory biomaterial for repairing injured areas where neovascularization is required.

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Endothelial differentiation of canine yolk sac cells transduced with VEGF

Cited by 18 publications

References 32 publications

Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells

Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells

Cochlear epithelial of dog fetuses: a new source of multipotent stem cells

Canine Placenta Recellularized Using Yolk Sac Cells with Vascular Endothelial Growth Factor

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