Endogenous steroid concentrations in human breast tumours determined by high-resolution mass fragmentography (Short Communication)

Abstract

A pilot study of the endogenous steroid concentrations in human breast tumours was performed. The technique of high-resolution molecular-ion monitoring during combined g.l.c.-mass spectrometry was used to determine oestrone, oestradiol-17beta and oestriol in concentrations above 1ng/g wet wt. of tissue, and dehydroepiandrosterone, testosterone, androsterone (3alpha-hydroxy-5alpha-androstan-17-one) and 3beta-hydroxy-5alpha-androstan-17-one in concentrations exceeding 5ng/g, in extracts of five primary breast tumours.

Epithelial and fibroblastoid cell lines derived from the normal canine prostate. II. Cell proliferation in response to steroid hormones

“…Analysis of steroids in fetal bovine serum. Samples of fetal bovine serum essential for the growth of both cell types in culture were analyzed by high resolution mass spectrometry [21] and radioimmunoassay [22], and their steroid content determined (Table I).…”

Evidence That the Deferential Vein Acts as a Local Transport System for Androgen in the Rat and the Dog

“…The extracts were evaporated to dryness, taken up in 30 µ bis-(trimethylsilyl) -acetamide and 20 µ light petroleum (60 to 80°C) in 1-ml screw-cap vials, and then allowed to stand overnight at 20°C. The method of gas chromatography-mass spectrometry using a Varian 2700 gas Chromatograph and Varian-MAT 731 mass spectro¬ meter has been described elsewhere (Millington, Jenner, Jones & Griffiths, 1974 Experiment 4. Six rats, which were not subjected to any surgical interference, were killed and the various lobes of the target organs were evaluated as described for incorporation into the analysis of variance of the animals which had been subjected to surgical procedures.…”

New techniques in quantitative mass spectrometry

“…A preliminary study using an external calibration procedure indicated a wide variety of oestradiol levels in different tumours, most being in the range of 0-5 to 10 pg/mg tissue wet weight (Millington, Jenner, Jones & Griffiths, 1974;Millington, 1975). The assay described earlier was designed to cover this range of values in a total of200 mg tissue sample.…”