Epithelial and fibroblastoid cell lines derived from the normal canine prostate. II. Cell proliferation in response to steroid hormones

Abstract

Epithelial and fibroblastoid cell lines derived from the adult canine prostate, have been used as model systems in a preliminary study of the effects of steroid hormones o n isolated cell populations. The mitogenic activities of various androgens and one estrogen were assessed during logarithmic growth in replicate subcultures by measurement of population size and radiolabeled nucleotide uptake in the presence of concentration ranges of these steroids.[H3]-Leucine incorporation rate was used as an estimate of protein synthesis in the epithelial cell line during the latter phase of log growth. Of the androgens examined, elevated cell population growth rate was only demonstrated in either epithelial or fibroblastoid cell cultures supplemented with 5a-androstane-3a. 170-and 1 7a-diols at physiological concentrations. No significant qualitative differences were observed between individual prostatic cell types in their proliferative response to the androgens tested. Contrasting effects were observed in the growth rates of epithelial and fibroblastoid cell types in cultures supplemented with estradiol-170. This estrogen was significantly mitogenic in fibroblast cultures, but did not significantly affect epithelial proliferation at the concentrations studied. These results are reviewed with reference to current concepts of steroid action in the canine prostate. The importance of the characterization of culture conditions and procedures has been emphasized as a prerequisite to the study of cell proliferation, and potential mitogens, using cell culture models.The relevance of these findings in the consideration of stromal-epithelial interaction, and glandular differentiation, is discussed.

Nonsteroidal serum factors involved in the regulation of the proliferation of canine prostatic epithelial cells in culture

“…It appears that cell proliferation and/or differentiation occurs in the presence of serum even in the absence of exogenous hormones. Eaton and Pierrepoint [33] have reported an additional stimulation of epithelial cell proliferation with 3a, 17@-diol and 3a, 17a-diol in monolayer cultures supplemented with FBS and insulin. The in vitro results described above, together with the data presented herein, are difficult to explain in view of the data obtained in vivo in the dog.…”

“…Initially, Sinowatz and Pierrepoint [31] and Sinowatz et a1 [32] have shown that in organ culture different I7a-hydroxy androgens activate the secretory epithelium and that only 3a, 17a-diol and 3/3, 17fi-diol can produce epithelial hyperplasia. More recently, Eaton and Pierrepoint [33] have demonstrated that some 170-as well as I7a-hydroxy androgens induce a proliferative response of the epithelial cells in organ culture.…”

Regulation of COX‐2 Expression in Canine Prostate Carcinoma: Increased COX‐2 Expression is Not Related to Inflammation

“…The nonmalignant canine prostate cell line CAPE 24,25 was a generous gift of Dr Tom Rosol (Columbus, OH), and the malignant cell line ACE was obtained from Dr Helen Jones (Cardiff, UK). Both cell lines were maintained at 37uC in an atmosphere of 5% CO 2 in Dulbecco's Modified Eagle's Medium f (DMEM) with the addition of 5% fetal calf serum f (FCS).…”

Steroid Hormones and the Pathogenesis of Benign Prostatic Hyperplasia

“…Both types also appear to have ER [21,22], although current data [19,27] suggest a much higher concentration in the stromal com partment of the human prostate.…”

“…Although ER may well be localized in the stromal elements of the human prostate, studies from this Institute [21,22], again using ligand-binding assays, con firmed the presence of ER in both epithelial and fibro blast cell lines derived from the normal canine prostate ( fig. 3).…”