Emulsification Properties of Biosurfactant Produced from Pseudomonas aeruginosa RB 28

Abstract

Biosurfactant produced from P. aeruginosa RB 28 was extracted, purified and characterized. Thin layer chromatography results showed that the extract contained two different compounds. The identification of the nature of the two compounds showed that they were glycolipids and rhamnose was the sugar moiety in these glycolipids. It was concluded that these compounds were rhamnolipids. The production of biosurfactant was started at late log phase and reached its maximal level (2.7 g L(-1)) at the stationary phase. Study of some rhamnolipid properties showed that sunflower oil, heptadecane and paraffin were efficiently emulsified and emulsions formed with vegetable oils (olive oil, corn oil and sunflower oil) were more stable than emulsions formed with hydrocarbons.

PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANT BY <i>Pseudomonas fluorescens</i> USING CASSAVA FLOUR WASTEWATER AS MEDIA

“…As comparisons, biosurfactant production by P. aeruginosa RB 28 (expressed as rhamnose equivalents) reached the maximum level at the stationary phase after 60 h of inuculation [48]. Production of biosurfactant from P. aeruginosa 44TI started after 14 h of inoculation and reached its maximal level after 58 h [49].…”

“…Emulsions formed with vegetable oils are more stable [48]. It was reported that rhamnolipids had the ability to emulsify efficiently hydrocarbons and vegetable oils [43].…”

Antimicrobial Activity of a Bioemulsifier Produced by Serratia marcescens S10

“…Sifour et al [15] reported that the bioemulsifier produced from P. aeruginosa with E.A about 0.43 for olive oil, and when n-alkanes was used E.A increased with the increasing of the number of carbon atoms.…”

“…and absorbency was measured at 540 nm. Emulsification activity was defined as the measured optical density; blank was Tris-Mg, dodecane and mineral salt broth with out culture [14,15].…”

Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

“…Then the cultures were centrifuged at 4°C, 10000 rpm, for 15 min. Biosurfactant was investigated in cell-free supernatant using three screening methods: Emulsification Activity (EA) (Sifour et al, 2007), emulsification index (E24%) (Tabatabaee et al, 2005) and surface tension which was measured by tensiometer (Krüss GmbH, Hamburg, Germany) using the du Nouy ring method.…”