PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANT BY &lt;i&gt;Pseudomonas fluorescens&lt;/i&gt; USING CASSAVA FLOUR WASTEWATER AS MEDIA

Suryanti, Venty; Marliyana, Soerya Dewi; Handayani, Desi Suci; Ratnaningrum, Desi

doi:10.22146/ijc.21281

Cited by 12 publications

(7 citation statements)

References 20 publications

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“…However, the variation of complex substrate’s composition between batches should be expected as its characteristic is fully dependent on the process and the raw material used. In particular, biosurfactant produced by P. fluorescens grown in media containing a mixture of natural manipueira (cassava flour wastewater) and nutrient broth reduced the surface tension of water to 59 mN/m from 80 mN/m compared to the media containing decanted manipueira [ 74 ]. Other agricultural waste like cashew apple juice (CAJ) was also utilized in biosurfactant production by Acinetobacter calcoaceticus .…”

Section: Media Components For Biosurfactant Productionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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Surfactants are compounds that can reduce the surface tension between two different phases or the interfacial tension of the liquid between water and oil, possessing both hydrophilic and hydrophobic moieties. Biosurfactants have traits that have proven to be advantageous over synthetic surfactants, but these compounds do not compete economically with synthetic surfactants. Different alternatives increase the yield of biosurfactants; development of an economical production process and the usage of cheaper substrates during process have been employed. One of the solutions relies on the suitable formulation of a production medium by including alternative raw materials sourced from agro-wastes, hydrocarbons, or by-products of a process might help in boosting the biosurfactant production. Since the nutritional factors required will be different among microorganisms, the establishment of a suitable formulation for biosurfactant production will be challenging. The present review describes various nutrients and elements considered in the formulation of a production medium with an approach focusing on the macronutrient (carbon, nitrogen source, and C/N ratio), minerals, vitamins, metabolic regulators, and salinity levels which may aid in the study of biosurfactant production in the future.

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Section: Media Components For Biosurfactant Productionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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“… [ 75 ] Yellow cashew P. aeruginosa 7.1–9.3 g/L [ 74 ] Cassava wastewater P. aeruginosa 169.9–300.3 mg/L [ 104 ] P. fluorescens N.A. [ 103 ] Pseudozyma tsukubaensis 8.11 g/L [ 105 ] Corncob hydrolysate Starmerella bombicola 33.7–49.2 g/L [ 141 ] B. subtilis BS-37 523 mg/L [ 142 ] Lactobacillus pentosus 0.53 g/g biomass [ 94 ] Corn flour Bacillus amyloliquefaciens XZ-173 1.56 mg/gds [ 139 ] Bacillus amyloliquefaciens XZ-173 8.38 mg/gds [ 140 ] Eucalyptus globulus chips Lactobacillus pentosus 0.54 g/g biomass [ 94 ] Raw cheese whey P. aeruginosa ATCC 10145 9.6 g/L [ 99 ] Paneer whey P. aeruginosa SR17 2.7–4.8 g/L [ 98 ] Whey ...…”

Section: Microbial Production Of Rhamnolipids Using Sugar-containing mentioning

confidence: 99%

“…Cassava flour wastewater mixed with nutrient broth was served as a medium for rhamnolipids production by P. fluorescens [ 103 ]. When solid residues were removed from cassava wastewater and autoclaved for use as a growth medium for various P. aeruginosa strains, rhamnolipids yields between 169.9 and 300.3 mg/L were achieved [ 104 ].…”

Section: Microbial Production Of Rhamnolipids Using Sugar-containing mentioning

confidence: 99%

Microbial production of rhamnolipids using sugars as carbon sources

Tan

2018

Microb Cell Fact

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Rhamnolipids are a class of biosurfactants with effective surface-active properties. The high cost of microbial production of rhamnolipids largely affects their commercial applications. To reduce the production post, research has been carried out in screening more powerful strains, engineering microbes with higher biosurfactant yields and exploring cheaper substrates to reduce the production cost. Extensive refining is required for biosurfactant production using oils and oil-containing wastes, necessitating the use of complex and expensive biosurfactant recovery methods such as extraction with solvents or acid precipitation. As raw materials normally can account for 10–30% of the overall production cost, sugars have been proven to be an alternative carbon source for microbial production of rhamnolipids due to its lower costs and straightforward processing techniques. Studies have thus been focused on using tropical agroindustrial crop residues as renewable substrates. Herein, we reviewed studies that are using sugar-containing substrates as carbon sources for producing rhamnolipids. We speculate that sugars derived from agricultural wastes rich in cellulose and sugar-containing wastes are potential carbon sources in fermentation while challenges still remain in large scales.

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“…The biosurfactants exhibited critical micelle concentration (CMC) value of 715 mg/L and lowered the surface tension of the water from 80 mN/m to 59 mN/m [12]. We also have found that soybean oil could be used as a carbon source for rhamnolipids biosurfactants by P. aeruginosa.…”

Section: Introductionmentioning

confidence: 82%

Heavy Metal Removal from Aqueous Solution Using Biosurfactants Produced by <i>Pseudomonas aeruginosa</i> with Corn Oil as Substrate

et al. 2018

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The batch removal of Cu(II), Cd(II) and Pb(II) adalah berturut-turut 0,169; 0,276; and 0,323 ABSTRAK Pengambilan ion logam Cu(II), Cd(II) dan Pb(II) dengan metode batch dari larutan menggunakan biosurfaktan yang diproduksi oleh Pseudomonas aeruginosa menggunakan minyak jagung sebagai substrat telah dipelajari. Proses pengambilan ion logam oleh biosurfaktan yang belum dimurnikan tergantung oleh pH awal dan waktu kontak. Pengambilan ion logam berat dalam larutan mempunyai kondisi optimum pada pH awal larutan 6,0 dan waktu kontak 10 menit. Urutan kekuatan pengambilan ion logam berat oleh CPB adalah Pb(II)>Cd(II)>Cu(II). Biosurfaktan mempunyai kapasitas pengambilan untuk logam Cu(II), Cd(II) dan Pb(II) dari larutan logam tunggal

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PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANT BY <i>Pseudomonas fluorescens</i> USING CASSAVA FLOUR WASTEWATER AS MEDIA

Abstract: Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira) as media.

Cited by 12 publications

References 20 publications

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Microbial production of rhamnolipids using sugars as carbon sources

Heavy Metal Removal from Aqueous Solution Using Biosurfactants Produced by <i>Pseudomonas aeruginosa</i> with Corn Oil as Substrate

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