Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

Al-Jailawi, Majid H.; Nas, Hiba Mansor; Azi, Ghazi Munaim

doi:10.3923/ajbkr.2015.96.107

Cited by 8 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell viability is one of the main tests that can be used in toxicity assays 24. Cell viability test indicates that there was a slight increase in cell viability when concentration of anastrozole was reduced.…”

Section: Resultsmentioning

confidence: 99%

“…Cell viability is one of the main tests that can be used in toxicity assays. 24 Cell viability test indicates that there was a slight increase in cell viability when concentration of anastrozole was reduced. The cell viability percentage was 88.6 when the concentration of anastrozole was 200, 95.3 (100), 99.9 (50) and 99.9 (25) µg/mL.…”

Section: Resultsmentioning

confidence: 99%

“…Toxicity of cells can lead to either necrosis or apoptosis along with other changes in nuclear morphology, mitochondrial function and cell permeability. 24 Cell toxicity can also lead to the loss of mitochondrial membrane potential and hence the release of cytochrome c from mitochondria. 24 In-vitro cytotoxicity test is designed to measure the lowest anastrozole dose that can cause cell toxicity.…”

Section: Discussionmentioning

confidence: 99%

“… 24 Cell toxicity can also lead to the loss of mitochondrial membrane potential and hence the release of cytochrome c from mitochondria. 24 In-vitro cytotoxicity test is designed to measure the lowest anastrozole dose that can cause cell toxicity. The changes in cell viability have a positive correlation with the toxic effect of the anastrozole tested.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Cytotoxicity anticancer activities of anastrozole against breast, liver hepatocellular, and prostate cancer cells

Hassan

El‐Hiti

Abd-Allateef

et al. 2017

SMJ

View full text Add to dashboard Cite

Objectives:To investigate the cytotoxic effect of anastrozole on breast (MCF7), liver hepatocellular (HepG2), and prostate (PC3) cancer cells.Methods:This is a prospective study. Anastrozole’s mechanism of apoptosis in living cells was also determined by high content screening (HCS) assay. Methylthiazol tetrazolium (MTT) assay was carried out at the Centre of Biotechnology Research’s, Al-Nahrain University, Baghdad, Iraq between July 2015 and October 2015. The HCS assay was performed at the Centre for Natural Product Research and Drug Discovery, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia between November 2015 and February 2016.Results:The most significant cytotoxic effect of anastrozole towards 3 cancer cell lines was obtained when its concentration was 400 µg/mL. The MCF7 cells were more sensitive to anastrozole compared with the HepG2 and PC-3 cells. There was a significant increase in membrane permeability, cytochrome c and nuclear intensity when anastrozole (200 µg/mL) was used compared with doxorubicin (20 µg/mL) as a standard. Also, there was a significant decrease in cell viability and mitochondrial membrane permeability when anastrozole (200 µg/mL) was used compared with positive control.Conclusion:Anastrozole showed cytotoxic effects against the MCF7, HepG2, and PC3 cell lines as determined in-vitro by the MTT assay. The HCS technique also showed toxic effect towards MCF7. It is evident that anastrozole inhibits the aromatase enzyme preventing the aromatization mechanism; however, it has a toxic effect.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cytotoxicity anticancer activities of anastrozole against breast, liver hepatocellular, and prostate cancer cells

Hassan

El‐Hiti

Abd-Allateef

et al. 2017

SMJ

View full text Add to dashboard Cite

show abstract

“…Cell viability is an important toxicity assay parameter and is directly associated with the toxic effects of a drug [34] . Tamoxifen induced a reduction in cell viability in MCF7 cells.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of tamoxifen in breast cancer cells

Hassan¹,

Jassam²,

El‐Hiti³

et al. 2018

JUMD

View full text Add to dashboard Cite

Aim: To investigate the cytotoxic effects of tamoxifen on the breast cancer cell line (MCF7). Methods:The cytotoxic effects of tamoxifen on MCF7 cells were investigated using caspase-9 activity and high content screening assays. Apoptosis mechanisms following tamoxifen treatment were also investigated. Results:The most significant cytotoxic effect of tamoxifen in MCF7 cells was a half-maximal inhibitory concentration (IC 50 ) of 4.506 µg/mL. A significant increase in caspase-9 activity was also observed when MCF7 cells were treated with tamoxifen (5 µg/mL). Furthermore, increased cell membrane permeability, cytochrome c level, and nuclear intensity were observed with tamoxifen (100 µg/mL) compared with doxorubicin (20 µg/mL) treatment. However, a noticeable decrease in cell viability and mitochondrial membrane permeability was observed with tamoxifen (100 µg/mL) treatment compared with doxorubicin (20 µg/mL) as a positive control. Conclusion:Tamoxifen showed in vitro cytotoxic effects in MCF7 cells as demonstrated by high-content screening and caspase-9 activity assays. Tamoxifen inhibits estrogen mechanisms, although toxic effect was observed.

show abstract

Effects of Defined Mixtures of Persistent Organic Pollutants (POPs) on Pre-lethal Cytotoxicity in the Human A-498 Kidney Cell Line In Vitro

et al. 2021

View full text Add to dashboard Cite

A total mixture of 29 persistent organic pollutants (POPs) modelled from Scandinavian blood concentrations was used to expose human A-498 kidney cells for 24 h over a concentration range spanning below to above blood level (1/10x, 1x, 50x, 100x, 500x). Its constituent submixtures (PFAA, Br, Cl) and co-mixtures (PFAA + Br, PFAA + Cl, Br + Cl) were also tested. Valinomycin (12 µM) was used as a cytotoxic comparative compound. Cell number (CN), nuclear area (NA), nuclear intensity (NI), mitochondrial membrane potential (MMP), and mitochondrial mass (MM) were assessed using high content analysis (HCA). Only the co-mixtures (PFAA + Cl, PFAA + Br) at 50x and 50x, 500x decreased CN, respectively. NI was increased by the total mixture at 500x and Cl mixture at all concentrations tested. MMP was increased by the total mixture at 100x and 500x, PFAA at 1x, Br + Cl and PFAA + Cl at 100x and 500x, respectively. MM was decreased by the total mixture at 500x. In contrast, valinomycin decreased CN and surviving cells showed a decrease in MMP and an increase in MM. In conclusion, POP exposure altered mitochondrial metabolism and induced cell death via an alternative mechanism to valinomycin. Only specific combinations of individual chemical classes, but not the total mixture, affected cell number.

show abstract

Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

Cited by 8 publications

References 30 publications

Cytotoxicity anticancer activities of anastrozole against breast, liver hepatocellular, and prostate cancer cells

Cytotoxicity anticancer activities of anastrozole against breast, liver hepatocellular, and prostate cancer cells

Cytotoxic effects of tamoxifen in breast cancer cells

Effects of Defined Mixtures of Persistent Organic Pollutants (POPs) on Pre-lethal Cytotoxicity in the Human A-498 Kidney Cell Line In Vitro

Contact Info

Product

Resources

About