Antimicrobial Activity of a Bioemulsifier Produced by Serratia marcescens S10

Abstract

This study was designed to evaluate the ability of bioemulsifier to inhibit the growth of some pathogenic microorganisms. Fourteen isolates belonged to Serratia sp. were collected and tested for their ability to produce bioemulsifier. Results showed that Serratia marcescens S10 (isolated from the gut of the American cockroach) had the highest ability to produce bioemulsifier, among 14 isolates belong to Serratia spp. and it had the ability to inhibit the growth of some microorganisms. The production of bioemulsifier was detected by determination of emulsification index (E24%), qualitative drop-collapse test, emulsification activity (E.A) and measuring the surface tension (S.T). The results of bioemulsifier produced by Serratia marcescens S10 cultivated in mineral salts broth containing olive oil were: E24%= 87%, E.A= 0.31 and reducing of surface tension from 65mN/m to 41.5mN/m. The bioemulsuifier was extracted by organic solvent (chloroform-methanol and diethylether) and antimicrobial activity (antibacterial and antifungal) of bioemulsifier was tested against some pathogenic microorganisms. Results revealed that bioemulsifier reduced the growth of bacteria (Lesteria spp., Salmonella spp., Klebsiella spp. and Staphylococcus aureus) and inhibited the growth of fungi (Candida albicans, Aspergillus niger and Geotricum spp.).

Production and Characterization of New Biosurfactants/Bioemulsifiers from Pantoea alhagi and Their Antioxidant, Antimicrobial and Anti-Biofilm Potentiality Evaluations

“…Here, the strain Zcb15 was able to produce BS showing antimicrobial and anti-biofilm potentialities. It has been reported that the BS produced by Bacillus species also have antibacterial action [ 27 , 38 ]. The current BS produced by Pantoea alhagi exhibited superior antimicrobial effect, with a ZI ranging from 13.75 to 36 mm as compared to those previously described [ 39 ], where ZI values did not exceed 9.64 mm.…”

Bioemulsifiers are not biosurfactants and require different screening approaches

“…Jain et al ( 2013 ) also reported on a bioemulsifier with a molecular weight of 2716 kDa, consisting of mainly total sugars, uronic acids and proteins produced by Klebsiella sp. On a general note bioemulsifiers have been associated with a number of potential applications including: remediation of oil polluted water and soil; enhanced oil recovery and clean-up of oil contaminated vessels and machineries; heavy metal removal (Monteiro et al, 2010 ; Zheng et al, 2012 ; Panjiar et al, 2014 ); formation of stable emulsions in food and cosmetics industries (Campos et al, 2014 ); and therapeutic activities (antibacterial, antifungal, pesticidal and herbicidal agents) (Ahmed and Hassan, 2013 ). The physico-chemical properties of bioemulsifiers and biosurfactants are presented in Table 1 .…”

Biosurfactants: Potential and Eco-Friendly Material for Sustainable Agriculture and Environmental Safety—A Review

“…Mannoproteins formed stable emulsions with almost all the compounds such as hydrocarbons, oils, etc., thus serves to be a promising class of biosurfactants to be applied in various industries [129]. Bioemulsifiers also serve to take a better place in the bioremediation of oil spills, heavy metal removal, food and agro industries, cosmetic industries, and pharmaceutical industries [127,[130][131][132][133]. Thus, these serve to be better solutions in these industries rather than the application of toxic synthetic surfactants [134].…”