Production and Characterization of New Biosurfactants/Bioemulsifiers from Pantoea alhagi and Their Antioxidant, Antimicrobial and Anti-Biofilm Potentiality Evaluations

Abstract

The present work aimed to develop rapid approach monitoring using a simple selective method based on a positive hemolysis test, oil spreading activity and emulsification index determinations. It is the first to describe production of biosurfactants (BS) by the endophytic Pantoea alhagi species. Results indicated that the new BS evidenced an E24 emulsification index of 82%. Fourier-transform infrared (FTIR) results mentioned that the described BS belong to the glycolipid family. Fatty acid profiles showed the predominance of methyl 2-hyroxydodecanoate in the cell membrane (67.00%) and methyl 14-methylhexadecanoate (12.05%). The major fatty acid in the BS was oleic acid (76.26%), followed by methyl 12-methyltetradecanoate (10.93%). Markedly, the BS produced by the Pantoea alhagi species exhibited antimicrobial and anti-biofilm activities against tested human pathogens. With superior antibacterial activity against Escherchia coli and Staphylococcus aureus, a high antifungal effect was given against Fusarium sp. with a diameter of zone of inhibition of 29.5 mm, 36 mm and 31 mm, obtained by BS dissolved in methanol extract. The DPPH assay indicated that the BS (2 mg/mL) showed a higher antioxidant activity (78.07 inhibition percentage). The new BS exhibited specific characteristics, encouraging their use in various industrial applications.

Heavy metal application of response surface optimized-lipopeptide biosurfactant produced by Pseudomonas aeruginosa strain CGA-02 in low-cost substrate

“…Thus, the components produced by P. aeruginosa strain CGA-02 are valuable materials, not only in bioremediation and sustainable environment, but in pharmaceuticals, biomedicals, food and cosmetic industries. The biosurfactant produced by P. aeruginosa strain CGA-02 are most similar to that reported in another study [70] in which oleic acid was recorded as the most dominant fatty acid component in biosurfactant produced by Pantoea alhagi (76.26% abundance). Other microorganisms such as E.coli [71] and Candida [72] can biosynthesize oleic acid, but the P. aeruginosa strain CGA-02 in this study, produced biosurfactant with relatively higher abundance of oleic acid (80.8%).…”

Screening of Microorganisms from Wastes and Identification of the Optimal Substrate for Biosurfactant Production

“…genus representatives could produce biosurfactants with an emulsification index exceeding 70%, thereby reducing the surface tension of the nutrient medium from 72 to 31 mN m −1 [35]. Additionally, Essghaier et al (2023) demonstrated that endophytes of Pantoea alhagi species are capable of producing biosurfactants with an emulsifying activity of 82% [36]. The species representative of B. tequilensis LK5.4 exhibited a maximum emulsification index of 52% [32].…”

Microbial Biosurfactants: Antimicrobial Activity and Potential Biomedical and Therapeutic Exploits

“…Biosurfactants comprise a group of unique amphiphilic molecules of microbial origin that are capable of interacting with lipidic components of microorganisms, altering their physicochemical properties [ 11 ]. It has been established that several of these compounds exhibit biological properties (antimicrobial and antifungal activity) [ 40 ] that make them potentially suitable for use in medical, pharmaceutical, and agricultural applications [ 41 ].…”