Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection

Dong, Ha Thanh; Siriroob, S.; Meemetta, Watcharachai; Santimanawong, Wanida; Gangnonngiw, Warachin; Pirarat, Nopadon; Khunrae, Pongsak; Rattanarojpong, Triwit; Vanichviriyakit, Rapeepun; Senapin, Saengchan

doi:10.1016/j.aquaculture.2017.04.019

Cited by 129 publications

(210 citation statements)

References 16 publications

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“…Virus ini merupakan genus dari famili Orthomyxoviridae, yang mereplikasi di inti sel pada jaringan ikan. TiLV yang pertama kali dilaporkan terjadi di Israel menyebar ke Ekuador dan Kolombia (Eyngor et al, 2014;Bacharach et al, 2016) dan ke beberapa negara seperti Mesir dan Nicholson et al, 2017, Thailand (Dong et al, 2017a;2017b;Surachetpong et al, 2017), serta India (Behera et al, 2017), serta Malaysia (Amal et al, 2017). Di Indonesia sejak beberapa tahun belakangan ini sering terjadi adanya kasus kematian massal budidaya ikan nila terutama di Sumatera, Jawa, Bali, dan Lombok.…”

Section: Studi Kasus Infeksi Tilapia Lake Virus (Tilv) Pada Ikan Nilaunclassified

“…Dengan demikian, kasus kematian ikan nila yang sering terjadi disentra budidaya dapat dideteksi secara dini agar mempermudah tindakan pencegahan. Tsofack et al (2016), amplifikasi dilakukan secara semi-nested RT-PCR (Dong et al, 2017a). Pada proses semi-nested RT-PCR dilakukan dengan menggunakan primers forward pada amplifikasi pertama.…”

Section: Studi Kasus Infeksi Tilapia Lake Virus (Tilv) Pada Ikan Nilaunclassified

“…Gejala klinis pada kasus kematian ikan nila di Lombok ini hampir sama seperti pada kasus kematian ikan nila yang terjadi di Israel dan Mesir, yaitu adanya kelainan pada mata, letargi atau lemas, serta gejala fisik lain seperti erosi kulit dan kematian massal lebih dari 80% (Eygnor et al, 2014). Gejala adanya infeksi TiLV di Thailand dilaporkan bahwa ikan nila kehilangan kemauan memangsa pakan, warna tubuh menjadi pucat, berkelompok di dasar bak, pergerakan lamban, tidak aktif, dan pada akhirnya mengalami mortalitas (Dong et al, 2017a). Kasus infeksi TiLV di Asia terutama di Thailand ternyata sudah ada sejak tahun 2012 ditunjukkan dari hasil analisis secara semi-nested RT-PCR, terdapat pada yolk-sac larvae red tilapia.…”

Section: Hasil Dan Bahasanunclassified

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STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

Koesharyani¹,

Gardenia

Widowati

et al. 2018

Jurnal Riset Akuakultur

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ABSTRAKIkan nila atau Oreochromis niloticus merupakan ikan konsumsi masyarakat di Indonesia. Kasus kematian massal ikan nila terjadi di beberapa lokasi budaya di Jawa, Lombok, dan Sumatera yang disebabkan oleh infeksi Orthomyxovirus, dan disebut sebagai Tilapia Lake Virus (TiLV). Tujuan penelitian ini adalah untuk mendeteksi adanya infeksi TiLV dengan metode semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) pada kasus kematian massal ikan nila. Lokasi pengambilan sampel di Desa Sigerongan Kecamatan Lingsar, Lombok, Nusa Tenggara Barat. Analisis deteksi RT-PCR menggunakan sampel organ otak, ginjal, limpa, dan hati, selanjutnya dilakukan sekuensing. Hasil pengamatan terhadap gejala klinis terhadap ikan nila moribund terlihat kondisi mata yang buram/katarak, serta cekung, abrasi kulit, serta perubahan warna tubuh menjadi lebih gelap. Hasil analisis RT-PCR menunjukkan bahwa kejadian kematian massal pada ikan nila suspektif diakibatkan oleh infeksi RNA virus TiLV. Analisis sekuensing menunjukkan bahwa TiLV dari sampel ikan nila di Lombok mempunyai kesamaan identitas genetik 97% dengan TiLV asal Israel (Genebank Accession Number KU 751816.1). KATA KUNCI: nila; TiLV; seminested RT-PCR ABSTRACT:Case study of tilapia lake virus (TiLV) infection on nila (Oreochromis niloticus

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Section: Studi Kasus Infeksi Tilapia Lake Virus (Tilv) Pada Ikan Nilaunclassified

Section: Hasil Dan Bahasanunclassified

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STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

Koesharyani¹,

Gardenia

Widowati

et al. 2018

Jurnal Riset Akuakultur

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“…In Israel, by contrast, tilapia infected with the tilapia lake virus (TiLV) were characterized by similarly high mortality (between 80%–100%), but by buphthalmia and encephalitis, and limited hepatic lesions (Eyngor et al., ). Several outbreaks of TiLV with mortalities reaching 90% have been reported in Thailand (Dong et al., ). In Egypt, the disease has been reported to cause summer mortalities (5%–15%) in numerous farms (Fathi et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…The identity of the associated virus in all of these distinct pathological presentations was shown to be an RNA virus (Eyngor et al., ; Tsofack et al., )‐related genetically (Bacharach et al., ) and morphologically to the Orthomyxoviridae family (del‐Pozo et al., ). There is now considerable information about the transboundary distribution, clinical presentation, gross pathology, histopathology and ultrastructural pathology of the disease, as well as characterization of the associated Orthomyxo‐like virus of tilapia (Bacharach et al., ; Dong et al., ; Eyngor et al., ; Ferguson et al., ; del‐Pozo et al., ; Tsofack et al., ), but we are aware of no work on epidemiological characteristics associated with its incidence and distribution. Knowledge of the epidemiological factors for SHT may contribute to the development of prevention and control strategies that should minimize the spread of the associated virus.…”

Section: Introductionmentioning

confidence: 99%

Production‐level risk factors for syncytial hepatitis in farmed tilapia (Oreochromis niloticus L)

Kabuusu

Aire

Stroup³

et al. 2017

Journal of Fish Diseases

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Vaccination of Tilapia against Motile Aeromonas Septicemia: A Review

et al. 2020

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The production of tilapia Oreochromis spp. is rapidly growing throughout the world, but atypical motile aeromonad septicemia (MAS) is a current threat to the tilapia farming industry. The etiological agent of this disease is usually Aeromonas hydrophila. Mortality rates due to MAS are frequently high, resulting in a devastating negative impact on this industry worldwide; therefore, proper control measures regarding both prevention and treatment are necessary. Although vaccines against MAS for tilapia are available, their effectiveness is entirely dependent on the specific strain of problematic bacteria. Until now, whole‐cell inactivated A. hydrophila vaccines for tilapia have exhibited the highest level of protection over live attenuated and recombinant vaccines. Among the various vaccine administration systems, only intraperitoneal (i.p.) injections of the A. hydrophila vaccine into tilapia were found to provide prominent immune protection. Vaccine efficacy was primarily measured by using the i.p. injection challenge model and estimating the relative percent survival of the immunized tilapia. Freund's incomplete adjuvant showed to be the most effective for tilapia MAS vaccines. In this review, multiple factors that directly or indirectly influence the efficacy of MAS vaccines for tilapia (adjuvants, challenge models, immunization doses and duration, and size of vaccinated fish) are discussed.

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Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection

Cited by 129 publications

References 16 publications

STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

Production‐level risk factors for syncytial hepatitis in farmed tilapia (Oreochromis niloticus L)

Vaccination of Tilapia against Motile Aeromonas Septicemia: A Review

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