ELISA for the serology of FIP virus

Osterhaus, Albert D. M. E.; Kroon, Anton I.P.M. de; Wirahadiredja, R. M. S.

doi:10.1080/01652176.1979.9693721

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…The ELISA has found wide application in the detection of antibodies and small amounts of antigen and seems to be particularly suitable for widespread epizootiological studies. The assay has been used for the detection of bovine coronavirus in feces (Ellens et al, 1978a) and for the serology of several coronaviruses, including feline infectious peritonitis virus (Osterhaus et al, 1979), murine hepatitis virus (Peters et al, 1979, Kraaijeveld et al, 1980a and human coronavirus strain 229E {Kraaijeveld et al, 1980a, b).…”

Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

Callebaut

Debouck

Pensaert

1982

Veterinary Microbiology

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An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected in PED.

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Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

Callebaut

Debouck

Pensaert

1982

Veterinary Microbiology

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“…The serologic tests are, however, only an aid to diagnosis because anti-coronavirus antibody can be detected not only in FIP cats but also in cats with other diseases and even in healthy animals [8,18,19,22,28,29]. The tests used routinely for detection of anti-coronavirus antibodies in felines are IFA or ELISA in which TGEV or CCV have been popularly used as the target antigen [17q9, 21]. The results in the present study indicate that the ELISA using CCV-infected cultured cells as antigens saves laborious purified viral antigen preparation, and it detects anti-coronavirus antibodies in cat sera more extensively than the MNT using CCV, and as efficiently as IFA using homologous FIPV.…”

Section: Discussionmentioning

confidence: 99%

An enzyme-linked immunosorbent assay using canine coronavirus-infected CRFK cells as antigen for detection of anti-coronavirus antibody in cat

Mochizuki

Furukawa

1989

Comparative Immunology, Microbiology and Infectious Diseases

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From the reasons that canine coronavirus (CCV) grows more efficiently than feline coronavirus in a cell culture and they are mutually related in their antigenicities, an enzyme-linked immunosorbent assay (ELISA) using CCV-infected feline kidney (CRFK) cells as substrate antigens was developed for detection of anti-coronavirus antibodies in cats. It was indispensable for generating coronavirus-specific ELISA antibody activities that the sample was applied to the mock-infected, normal CRFK cells in parallel with the CCV-infected cells and then the optical density values given by the mock-infected cell antigen were subtracted from those given by the virus-infected cell antigen. On the basis of ELISA antibody titers obtained in sera from the cats experimentally infected with CCV and from the spontaneous feline infectious peritonitis (FIP) cases, the ELISA described in the present study was found to be applicable as a simple and easy serologic test which was able to detect anti-coronavirus antibodies as efficiently as the indirect immunofluorescence assay with homologous FIP virus.

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“…Fixative and coupling agents for improving antigen retention on support media, such as formaldehyde (12,25), glutaraldehyde (1,55,64), acetone (57), and poly-L-lysine (14,37) have also been used. Most ELISA protocols for detection of coronavirus antibodies thus far published (including that of Osterhaus et al [36] for coronavirus antibodies in cats) have used carbonatebicarbonate or borate buffer diluents at pH 9.2 to 9.8 for antigen adsorption, without additional chemical modification (9,15,24,30,36,45,46,58). Both infected culture fluids and gradientpurified coronavirus have been used as antigens in these assays.…”

Section: Discussionmentioning

confidence: 99%

“…The humoral immune response of cats to a coronavirus(es) has been studied by using sever-al techniques, including indirect immunofluorescence assay (IFA) (26,35,40,48), enzymelinked immunosorbent assay (ELISA) (36,41), virus neutralization (19,28,49,50,60), and passive hemagglutination (60). Because feline infectious peritonitis virus has until recently been impossible to propagate in conventional monolayer cell cultures, most of these assays have relied upon the antigenic relationship which exists between feline infectious peritonitis virus and certain less fastidious coronaviruses, such as transmissible gastroenteritis virus (TGEV) of swine, canine coronavirus (CCV), and human respiratory coronavirus 229E (35,44,49,51,70).…”

mentioning

confidence: 99%

“…Because feline infectious peritonitis virus has until recently been impossible to propagate in conventional monolayer cell cultures, most of these assays have relied upon the antigenic relationship which exists between feline infectious peritonitis virus and certain less fastidious coronaviruses, such as transmissible gastroenteritis virus (TGEV) of swine, canine coronavirus (CCV), and human respiratory coronavirus 229E (35,44,49,51,70). Although IFA is the only one of these procedures to have been widely utilized thus far in clinical veterinary serology, ELISA has shown excellent potential as a rapid and sensitive method for detection of coronavirus antibodies in cats (2,36).…”

mentioning

confidence: 99%

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Evaluation of a computer-assisted, kinetics-based enzyme-linked immunosorbent assay for detection of coronavirus antibodies in cats

Barlough¹,

Jacobson²,

Downing³

et al. 1983

J Clin Microbiol

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A computer-assisted, kinetics-based enzyme-linked immunosorbent assay was adapted for the detection of coronavirus antibodies in feline serum. An alkaline antigen diluent (carbonate-bicarbonate buffer, pH 9.6) used in initial experiments produced diffuse, nonspecific color reactions in both viral and control antigen cuvettes which were correlated, paradoxically, with coronavirus antibody levels in test sera. These interfering reactions were minimized by use of lower-pH antigen diluents such as water and phosphate-buffered saline. Background kinetics-based enzyme-linked immunosorbent assay reactivity directed against a noncoronaviral component of antigen tissue culture fluids could then detected in numerous sera, particularly in samples with lower titers. Much of this reactivity was shown to be associated with bovine gamma globulins in cell culture fluid. It was not serum lot or species specific, since a variety of bovine serum lots as well as individual lots of serum from other mammalian and avian species reacted. Reactivity was markedly reduced when cells for antigen preparation were grown in gamma globulin-free bovine serum. Generation of corrected slope values from the kinetics-based enzyme-linked immunosorbent assay made it possible to correct for residual background reactivity in individual test sera and thus eliminate a potentially major source of false-positive reactions. Collectively, these studies indicated that the control of nonspecific reactivity in feline coronavirus serology is absolutely essential to obtain useful estimates of specific antibody responses.

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ELISA for the serology of FIP virus

Cited by 7 publications

References 6 publications

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

An enzyme-linked immunosorbent assay using canine coronavirus-infected CRFK cells as antigen for detection of anti-coronavirus antibody in cat

Evaluation of a computer-assisted, kinetics-based enzyme-linked immunosorbent assay for detection of coronavirus antibodies in cats

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