Eletrocromatografia capilar: contextualização, estado da arte e perspectivas

Segato, Milena Pinotti; Silva, César Ricardo; Jardim, Isabel Cristina Sales Fontes

doi:10.1590/s0100-40422009000200030

Cited by 8 publications

(7 citation statements)

References 81 publications

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“…Technical innovations for interfacing CE with flow and sequential injection analysis (SIA) as sampling means have been addressed in three complementary review papers 8–10. Updated insights into separation modes that are generally less common for inorganic analytes, such as MEKC 11, MEEKC 12, NACE 13, CEC 14, or based on using ionic liquids as BGE additives 15, 16, revealed that these are still limited to solve selectivity (and solubility) issues.…”

Section: Reviewsmentioning

confidence: 99%

CE of inorganic species – A review of methodological advancements over 2009–2010

Kubáň

Timerbaev

2011

Electrophoresis

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This article is the seventh in a series examining biannually the methodological developments in the field of CE analysis of inorganic species and covers relevant documents published between January 2009 and December 2010. Following an analysis of the significant accomplishments that have impacted the field in two recent years, a survey of advances in general CE methodology is presented. Subsequently, several notable trends that can be perceived in this well-established field are discussed: the continuing rise of ME and consequent development of suitable detection techniques, most notably contactless conductivity detection, the constant pace of advances in speciation analysis, and an increase in non-analytical CE applications to study complexation and (bio)transformation reactions of metal analytes. A range of recently emerged multi-detection designs, ICP-MS interface devices, and separation systems, for which outpacing work has been conducted, are also brought into focus.

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Section: Reviewsmentioning

confidence: 99%

CE of inorganic species – A review of methodological advancements over 2009–2010

Kubáň

Timerbaev

2011

Electrophoresis

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“…CEC is an emerging and promising technique, which combines the high selectivity of HPLC and the high efficiency of CE, and has shown great potential for analyzing complex solute mixtures . Packing columns, monolithic columns, and open‐tubular columns are commonly used in CEC.…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide and reduced graphene oxide as novel stationary phases via electrostatic assembly for open‐tubular capillary electrochromatography

Liu

et al. 2013

Electrophoresis

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We present here the application of graphene oxide (GO) and reduced graphene oxide (GOOH) sheet as novel stationary phases for open-tubular CEC (OTCEC) separation based on electrostatic assembly. The inner walls of a bare capillary column was first modified by ionic assembly of poly (diallyldimethylammonium chloride) (PDDA), and then negatively charged GO or GOOH was easily assembled on a positively charged interior walls of the capillary by electrostatic force. Scanning Electron Microscope images showed that GO and GOOH can still maintain sheet-layer-like structure when coated onto the capillary via electrostatic assembly. The chromatographic properties of the GO and GOOH coated columns were evaluated via OTCEC separations of various kinds of analytes, including three acid nitrophenol isomers, three basic nitroaniline isomers, and four neutral PAHs. Efficient separations of all the analytes were achieved with optimized buffer pH and organic additive. The reproducibility and stability of the GO or GOOH coated columns were investigated. Our results indicate the capability of application GO or GOOH sheet in OTCEC separation, which can be coated on the inner wall of fused-silica capillary via electrostatic assembly.

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“…These columns present the disadvantage of a lower loading capacity due to the smaller area of the stationary phase and the unfavorable mobile phase to stationary phase volume ratio relative to particulate and monolithic columns. 17 Due to the disadvantages observed with particulate and wall-coated open-tubular columns, the development of monolithic capillary columns containing extremely porous and easily synthesized material is of great current interest. The monoliths are prepared by polymerization processes in situ in a single step that also allows the grafting of charged species into the stationary phase, fundamental for assuring the electrosmotic flow.…”

Section: Introductionmentioning

confidence: 99%

Physical and electrophoretic characterization of octadecyl methacrylate-based monolithic columns for use in capillary electrochromatography

Aguiar¹,

Bottoli²

2013

J. Braz. Chem. Soc.

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Colunas de alta eficiência cromatográfica de origem monolítica orgânica foram obtidas para uso em eletrocromatografia capilar (CEC) utilizando uma mistura de monômeros (concentrações constantes) com diferentes proporções de solventes porogênicos (1,4-butanodiol com álcool isoamílico, álcool amílico ou cicloexanol, na presença ou ausência de água). As fases estacionárias foram preparadas a partir do monômero precursor octadecilmetacrilato (ODMA), do agente de entrecruzamento etilenodimetacrilato (EDMA) e do monômero carregado ácido 2-acriloilamido-2-metilpropanosulfóxido (AMPS), sendo este necessário para tornar a fase estacionária carregada negativamente, garantindo o fluxo eletrosmótico durante a análise. As colunas monolíticas foram caracterizadas fisicamente através de porosimetria e microscopia eletrônica de varredura (SEM), e eletrocromatograficamente, através de cálculo dos parâmetros cromatográficos de separação. A coluna mais eficiente foi preparada a partir dos solventes porogênicos álcool amílico:1,4-butanodiol na proporção 65:35 (v/v) e apresentou 38 µm de altura de prato. Estas colunas foram aplicadas na separação de alquilparabenos e hidrocarbonetos policíclicos aromáticos (PAH).Organic monolithic columns with high efficiency were obtained for use in capillary electrochromatography (CEC) from a mixture of monomers (constant concentrations) with different proportions of porogenic solvents (1,4-butanediol with isoamyl alcohol, amyl alcohol or cyclohexanol in the presence or absence of water). The stationary phases were prepared from the precursor monomer octadecyl methacrylate (ODMA), the cross-linking agent ethylene dimethacrylate (EDMA) and the ionizable monomer 2-acryloylamido-2-methylpropanesulfonic acid (AMPS), the latter being necessary to make the stationary phase negatively charged, assuring electrosmotic flow (EOF) during analysis. The monolithic columns synthesized were physically characterized by porosimetry and scanning electron microscopy (SEM), and electrochromatographically characterized by calculation of chromatographic parameters. The most efficient column was prepared using the porogenic solvent amyl alcohol:1,4-butanediol in a 65:35 (v/v) ratio and showed a plate height of 38 µm. These columns were applied for the separation of alkyl parabens and polycyclic aromatic hydrocarbons (PAH). Keywords: capillary electrochromatography, monolithic stationary phases IntroductionCapillary electrochromatography (CEC) is a hybrid technique of capillary electrophoresis (CE) and high performance liquid chromatography (HPLC).1,2 The CEC technique can be defined as a type of capillary electrophoresis carried out in packed capillary columns in an environment in which an applied electric field is responsible for the movement of the mobile phase across the capillary, generating the electrosmotic flow (EOF).3 Different from separations in zone capillary electrophoresis, the use of the stationary phase results in the separation of uncharged sample components. The major advantage of CEC compared to HPLC i...

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Eletrocromatografia capilar: contextualização, estado da arte e perspectivas

Cited by 8 publications

References 81 publications

CE of inorganic species – A review of methodological advancements over 2009–2010

CE of inorganic species – A review of methodological advancements over 2009–2010

Graphene oxide and reduced graphene oxide as novel stationary phases via electrostatic assembly for open‐tubular capillary electrochromatography

Physical and electrophoretic characterization of octadecyl methacrylate-based monolithic columns for use in capillary electrochromatography

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