Electrospun polylactic acid/sulfadiazine sodium/proteinase nanofibers and their applications in treating frostbite

Abstract

In this study, polylactic acid (PLA)/sulfadiazine sodium/proteinase K composite nanofibers were prepared by electrospinning, and their morphology was observed by scanning electron microscope. The novel nanofibers were characterized by Fourier transform infrared spectroscopy and drug release curve. The activity of different groups of drugs loaded nanofibers in frostbite wound of rats was studied and compared with the blank control group. Hematoxylin-eosin staining and transmission electron microscope were used to access the histological changes of frostbite wounds on the back of rats before and after treatment.CD31 fluorescence staining was used to track the reconstruction and improvement of blood vessels in tissues. The results revealed that the drug release rate of PLA/sulfadiazine sodium/proteinase K nanofibers group was more than 50% at 1 h, and the cumulative drug release rate was more than 88% at 168 h, and CD31 fluorescence showed that its vascular density was 13.27% ± 0.9%, which was significantly higher than that of other groups. Therefore, PLA/sulfadiazine sodium/proteinase K composite nanofibers could promote the healing of frostbite wounds, which indicated the increase of capillaries and the regeneration of hair follicles. PLA/sulfadiazine sodium/proteinase K composite nanofibers have great potential in the application of frostbite.

Pultrusion preparation and properties of continuous glass fiber reinforced polylactic acid thermoplastic composites

“…Polylactic acid (PLA) is a kind of thermoplastic polymer produced from lactic acid, which has been successfully utilized in biodegradable material and as a composite matrix material. [30][31][32][33][34] In addition, PLA is a promising and prominent biomaterial that has been widely used to replace the traditional petrochemicalbased polymers in various applications because of the environmental concerns and relatively good mechanical properties. 35 Especially green thermoplastic composites have gained greater attention as ecological consciousness has grown to be more appealing than conventional toxic and nonbiodegradable petroleum-based composites.…”

“…Polylactic acid (PLA) is a kind of thermoplastic polymer produced from lactic acid, which has been successfully utilized in biodegradable material and as a composite matrix material 30–34 . In addition, PLA is a promising and prominent biomaterial that has been widely used to replace the traditional petrochemical‐based polymers in various applications because of the environmental concerns and relatively good mechanical properties 35 .…”

Electrospun Structural Hybrids of Acyclovir-Polyacrylonitrile at Acyclovir for Modifying Drug Release

“…For example, the immediate release of a drug is essential when rapid action is required, and is welcomed by patients with conditions such as pains or fevers and heart attacks [34][35][36][37][38]. Alternatively, sustained drug release for a long time period is popular with patients for cases where the administration frequency can be reduced for a better quality of life [39][40][41][42][43][44]. Double-stage release, which often includes a fast release followed by an extended release, can combine the advantages of both of these approaches [31][32][33][34][35].…”