Electrodeposition of Co Nanoparticles on the Carbon Ionic Liquid Electrode as a Platform for Myoglobin Electrochemical Biosensor

Abstract

The electrochemical deposition of Co nanoparticles on carbon ionic liquid electrode (CILE) was described and further used as the platform to construct a myoglobin (Mb) electrochemical biosensor. CILE was prepared by mixing a certain ratio of carbon powder, 1-ethyl-3-methylimidazolium tetrafluoroborate (EMIMBF 4 ), and liquid paraffin together. The presence of ionic liquid on the electrode surface facilitated the formation of Co nanoparticles, and a layer of Co nanoparticles was deposited on the surface of CILE with the average diameter of 300 nm after the cyclic voltammetic scan in the CoCl 2 solution. The formed Co/CILE was used as a new basal electrode for the investigation on the direct electrochemistry of protein. Mb molecules were further cast on the surface of Co/CILE and immobilized with Nafion film. The fabricated Nafion/Mb/Co/CILE showed good electrochemical behaviors with a pair of well-defined quasi-reversible redox peaks of Mb obtained, which was attributed to the electrochemical reaction of heme Fe(III)/Fe(II) redox couples. The results indicated that the presence of Co nanoparticles exhibited great promotion to the direct electron transfer of Mb. The Mb electrochemical biosensor showed good electrocatalytic activity to the reduction of hydrogen peroxide and trichloroacetic acid (TCA). The modified electrodes showed good stability and reproducibility, which had potential application in third generation biosensor.

A Novel Nitrite Biosensor Based on Gold Dendrites with Egg White as Template

“…The result was 1.49 s -1 , which was higher than that 0.332 s -1 of the Nafion/Mb/multi-walled carbon nanotubes/carbon ionic liquid electrode (CILE) 22 and 0.588 s -1 of the Co/CILE. 23 Thus, the Mb-Cys-AuD bio-hybrid can provide a favorable microenvironment for Mb to undergo a facile electron transfer reaction for two reasons: (1) the structure of AuD will promote effective immobilization of enzyme, protein and other bioactive substances. Indeed, the dendrites structure greatly increased the specific surface area and offered more binding sites for the immobilization of Mb; (2) the soft template egg white contains a lot of protein and amino acid, which provide a benign microenvironment for the assembly process.…”

“…In Table 1, some of the analytical characteristics obtained in this work are compared with those previously reported in the literature. 23,[29][30][31][32][33][34] It can be seen that the responses of the Mb-Cys-AuD/GCE are, in most cases, superior, especially the linear range and the detection limit, to the values obtained previously using the NO2 -biosensor.…”

Nanostructured Mimic Enzymes for Biocatalysis and Biosensing

“…As a typical example, they catalyzed the transamination between an amino acid and a keto acid, which is the most important form of nitrogen transfer in diverse biological systems. 3 6 PB can be electrochemically reduced to Prussian white (PW), which is capable of catalyzing the reduction of H 2 O 2 at low potentials. The mechanism is as follows: PB has been intensively investigated in the form of thin polycrystalline electrodeposited films.…”

A novel biosensor based on electro-co-deposition of sodium alginate-Fe3O4-graphene composite on the carbon ionic liquid electrode for the direct electrochemistry and electrocatalysis of myoglobin

“…However, because of the deeply embedded redox active center in the structure of proteins, the direct electron transfer between the redox proteins and the electrode is hard to be achieved [5,6]. For this reason, it is necessary to conduct the modification of the substrate electrode by the use of different methods and materials, such as electro-co-deposition [7], sol-gel [8], self-assembly [9], covalent binding [10], and polymers [11,12], inorganic materials [13,14], metal oxides nanoparticles [15,16], etc., to facilitate the electron transfer rate between the active center of proteins with the electrode. For example, Abu-Rabeah et al [17] created a highly responsive glucose biosensor by covalent attachment of glucose oxidase with alginate-pyrrole matrix, followed by pyrrole polymerization.…”