Electrochemical impedance detection of DNA hybridization based on dendrimer modified electrode

Li, Aixue; Yang, Fan; Ma, Ying; Yang, Xiurong

doi:10.1016/j.bios.2006.07.033

Cited by 144 publications

(66 citation statements)

References 30 publications

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“…6B-b). These results clearly presented that the modification of carbon nanotubes onto the graphite surface improved the immobilization capacity of dsDNA similarly to the earlier reports using nanomaterials [15,34]. Correspondingly to the surface confined biomolecular interaction with cis-DDP, an increase level was found 32.4% at the R ct value, that may possibly indicate that there was a specific binding of platinum complexes to the guanine bases of in the double helix form of DNA.…”

Section: Resultssupporting

confidence: 76%

Electrochemical investigation of biomolecular interactions between platinum derivatives and DNA by carbon nanotubes modified sensors

Yapasan

Caliskan

Karadeniz

et al. 2010

Materials Science and Engineering: B

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a b s t r a c tThe biomolecular interactions of platinum derivatives widely used as anticancer drugs: cisdiamminedichloroplatinum(II) and oxaliplatin with calf thymus double-stranded DNA were studied using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) in combination with single-walled carbon nanotubes modified graphite electrode (SWCNTs-GE) and unmodified graphite electrode (bare GE). The performance of these biomolecular interactions were explored at the electrode surface by monitoring the changes at guanine oxidation signal in terms of optimum interaction times by comparing the results of SWCNTs-GE with bare one. The features of these electrochemical sensors based on carbon nanotubes for monitoring of biomolecular interactions were discussed and compared with the earlier conventional ones.

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Section: Resultssupporting

confidence: 76%

Electrochemical investigation of biomolecular interactions between platinum derivatives and DNA by carbon nanotubes modified sensors

Yapasan

Caliskan

Karadeniz

et al. 2010

Materials Science and Engineering: B

View full text Add to dashboard Cite

show abstract

“…8Bb). These results clearly presented that the modification of carbon nanotubes onto the graphite surface improved the immobilization capacity of dsDNA similarly to the ones obtained by using DNA immobilized dendrimer modified electrode [38].…”

Section: Resultssupporting

confidence: 64%

Single‐Walled Carbon Nanotubes Modified Graphite Electrodes for Electrochemical Monitoring of Nucleic Acids and Biomolecular Interactions

2009

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Single-walled carbon nanotubes (SWCNT) modified disposable graphite electrodes (SWCNT-PGEs) were investigated in our study for the improved electrochemical monitoring of nucleic acids and biomolecular interactions based on the higher signal enhancement comparison to bare PGEs. The surface morphologies of bare PGE and SWCNT-PGE were firstly explored using scanning electron microscopy (SEM) analysis. The easy surface modification of disposable graphite electrodes with carbon nanotubes was performed by passive adsorption, and DNA was then immobilized onto the SWCNT-PGEs by the formation of covalent coupling between the carboxylated ends of nanotubes and the amine group in the guanine bases of DNA. The overall performance of SWCNT-PGEs has also been studied, and discussed in terms of optimum analytical conditions; such as, the effect of pretreatment step, CNT concentration, DNA concentration, etc. The reproducible detection of DNA represent a very attractive approach for the further detection of interaction between the anticancer drug, daunorubicin (DNR) and double stranded DNA (dsDNA). Voltammetric results were complemented with electrochemical impedance spectroscopy (EIS), that was used to characterize the successful construction of carbon nanotubes modification onto the surface of PGEs.

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“…The larger R ct value in almost sevenfolds (shown in Fig. 4Bb) can be similarly explained [43] that DNA probe prevented redox couple, [Fe(CN) 6 ] 3À/4À from reaching the electrode surface due to the fact that the charge repulsion between the negatively charged phosphate backbone of oligonucleotide and [Fe(CN) 6 ] 3À/4À resulted in a reduced ability for electron transfer at the electrode surface.…”

Section: Resultsmentioning

confidence: 65%

“…In the Nyquist plot of impedance spectra, the semicircle section at higher frequencies characterize the electron transfer limited process and the linear section seen at lower frequencies may be attributed to the diffusion [42]. After the modification of the PGE surface with SWCNT, the average R ct value (shown in Figure 4Ba) was calculated as 132.3 W, which is six times larger than the one obtained by bare PGE (not shown) in parallel to the results obtained by using bare gold electrode and multiwalled carbon nanotube modified one [43]. After CNT modification step, this change obtained at the R ct value has been a strong verification that CNT had been immobilized onto PGE surface.…”

Section: Resultsmentioning

confidence: 97%

Direct DNA Hybridization on the Single‐Walled Carbon Nanotubes Modified Sensors Detected by Voltammetry and Electrochemical Impedance Spectroscopy

2009

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Carboxylic acid functionalized single-walled carbon nanotubes modified graphite sensors (SWCNT-PGEs) were developed for electrochemical monitoring of direct DNA hybridization related to specific sequence of Hepatitis B virus, which substantially enhance the electrochemical transduction resulting from guanine oxidation signal comparison to bare PGEs. The performance characteristics of DNA hybridization on disposable CNT-PGE were explored measuring the guanine signal in terms of optimum analytical conditions; probe and target concentration, hybridization time, and selectivity. The voltammetric results were also complemented with electrochemical impedance spectroscopy (EIS), that was used to characterize the successful construction of carbon nanotubes modification onto the surface of PGEs.

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Electrochemical impedance detection of DNA hybridization based on dendrimer modified electrode

Cited by 144 publications

References 30 publications

Electrochemical investigation of biomolecular interactions between platinum derivatives and DNA by carbon nanotubes modified sensors

Electrochemical investigation of biomolecular interactions between platinum derivatives and DNA by carbon nanotubes modified sensors

Single‐Walled Carbon Nanotubes Modified Graphite Electrodes for Electrochemical Monitoring of Nucleic Acids and Biomolecular Interactions

Direct DNA Hybridization on the Single‐Walled Carbon Nanotubes Modified Sensors Detected by Voltammetry and Electrochemical Impedance Spectroscopy

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