Abstract:a b s t r a c tThe biomolecular interactions of platinum derivatives widely used as anticancer drugs: cisdiamminedichloroplatinum(II) and oxaliplatin with calf thymus double-stranded DNA were studied using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) in combination with single-walled carbon nanotubes modified graphite electrode (SWCNTs-GE) and unmodified graphite electrode (bare GE). The performance of these biomolecular interactions were explored at the electrode surfa… Show more
“…As the highest increase at guanine signal was found in the ratio of 65.4 % by 3000 mg/mL GRPox modified PGE, the further experiments were studied in this concentration level of GRPox. This result was also found parallel to the ones of single walled carbon nanotubes based disposable graphite electrodes (SWCNT-PGEs) developed for DNA hybridization and DNA-drug interactions [40][41][42].…”
In this paper, the application of a Graphene oxide (GRPox) integrated on single-use pencil graphite electrode (PGE) for the enhanced monitoring of nucleic acids and for the sensitive and selective detection of the label free DNA hybridization related to Hepatitis B virus (HBV) sequences is described. Electrochemical behaviors of GRPox modified PGEs were firstly investigated using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The sequence selective DNA hybridization was determined voltammetrically in the case of hybridization between amino linked probe and its complementary (target), or, noncomplementary (NC), or target/mismatch (MM) mixtures (1 : 1).
“…As the highest increase at guanine signal was found in the ratio of 65.4 % by 3000 mg/mL GRPox modified PGE, the further experiments were studied in this concentration level of GRPox. This result was also found parallel to the ones of single walled carbon nanotubes based disposable graphite electrodes (SWCNT-PGEs) developed for DNA hybridization and DNA-drug interactions [40][41][42].…”
In this paper, the application of a Graphene oxide (GRPox) integrated on single-use pencil graphite electrode (PGE) for the enhanced monitoring of nucleic acids and for the sensitive and selective detection of the label free DNA hybridization related to Hepatitis B virus (HBV) sequences is described. Electrochemical behaviors of GRPox modified PGEs were firstly investigated using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The sequence selective DNA hybridization was determined voltammetrically in the case of hybridization between amino linked probe and its complementary (target), or, noncomplementary (NC), or target/mismatch (MM) mixtures (1 : 1).
“…Each pencil lead was immersed into the vial tubes containing 100 mL of 3000 μg/mL SWCNT solution during 1 hour in order to form a thin SWCNT layer on the surface of electrodesvia passive adsorption, as previously reported in our earlier studies . Each of these electrodes was rinsed with ABS for 10 seconds, and then SWCNT modified PGEs were allowed to dry during 15 min at upside down position .…”
In this present study, single‐walled carbon nanotubes (SWCNT) modified disposable pencil graphite electrodes (SWCNT‐PGEs) were developed for the electrochemical monitoring of anticancer drug, and its interaction with double stranded DNA (dsDNA). Under this aim, SWCNT‐PGEs were applied for the first time in the literature to analyse of 6‐Thioguanine (6‐TG), and also to investigate its interaction with DNA by voltammetric and impedimetric methods. The surface morphologies of PGE and SWCNT‐PGE were explored using scanning electron microscopy (SEM) and electrochemical characterization of unmodified/modified electrodes was performed by cyclic voltammetry (CV). Experimental parameters; such as, the concentration of 6‐TG and its interaction time with dsDNA were optimized by using differential pulse voltammetry (DPV). Additionally, the interaction of 6‐TG with dsDNA was studied in case of different interaction times by electrochemical impedance spectroscopy (EIS) in contrast to voltammetric results. The detection limit of 6‐TG was found to be 0.25 μM by SWCNT‐PGE.
“…A rapid, reproducible and sensitive method is also developed for the biomolecular recognition of cis-diamminedichloroplatinum, an anticancer drug using SWCNT modified disposable graphite electrodes. SWCNTs are capable of enhancing the guanine oxidation signal (Yapasan et al, 2010) of differential pulse voltammetry and EC impedance spectroscopy. Another anticancer agent oxaliplatin has also also been tested.…”
Electrochemical (EC) sensing approaches have exploited the use of carbon nanotubes (CNTs) as electrode materials owing to their unique structures and properties to provide strong electrocatalytic activity with minimal surface fouling. Nanofabrication and device integration technologies have emerged along with significant advances in the synthesis, purification, conjugation and biofunctionalization of CNTs. Such combined efforts have contributed towards the rapid development of CNT-based sensors for a plethora of important analytes with improved detection sensitivity and selectivity. The use of CNTs opens an opportunity for the direct electron transfer between the enzyme and the active electrode area. Of particular interest are also excellent electrocatalytic activities of CNTs on the redox reaction of hydrogen peroxide and nicotinamide adenine dinucleotide, two major by-products of enzymatic reactions. This excellent electrocatalysis holds a promising future for the simple design and implementation of on-site biosensors for oxidases and dehydrogenases with enhanced selectivity. To date, the use of an anti-interference layer or an artificial electron mediator is critically needed to circumvent unwanted endogenous electroactive species. Such interfering species are effectively suppressed by using CNT based electrodes since the oxidation of NADH, thiols, hydrogen peroxide, etc. by CNTs can be performed at low potentials. Nevertheless, the major future challenges for the development of CNT-EC sensors include miniaturization, optimization and simplification of the procedure for fabricating CNT based electrodes with minimal non-specific binding, high sensitivity and rapid response followed by their extensive validation using "real world" samples. A high resistance to electrode fouling and selectivity are the two key pending issues for the application of CNT-based biosensors in clinical chemistry, food quality and control, waste water treatment and bioprocessing.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.