Direct DNA Hybridization on the Single‐Walled Carbon Nanotubes Modified Sensors Detected by Voltammetry and Electrochemical Impedance Spectroscopy

Abstract

Carboxylic acid functionalized single-walled carbon nanotubes modified graphite sensors (SWCNT-PGEs) were developed for electrochemical monitoring of direct DNA hybridization related to specific sequence of Hepatitis B virus, which substantially enhance the electrochemical transduction resulting from guanine oxidation signal comparison to bare PGEs. The performance characteristics of DNA hybridization on disposable CNT-PGE were explored measuring the guanine signal in terms of optimum analytical conditions; probe and target concentration, hybridization time, and selectivity. The voltammetric results were also complemented with electrochemical impedance spectroscopy (EIS), that was used to characterize the successful construction of carbon nanotubes modification onto the surface of PGEs.

Electrochemical Monitoring of Nucleic Acid Hybridization by Single‐Use Graphene Oxide‐Based Sensor

“…As the highest increase at guanine signal was found in the ratio of 65.4 % by 3000 mg/mL GRPox modified PGE, the further experiments were studied in this concentration level of GRPox. This result was also found parallel to the ones of single walled carbon nanotubes based disposable graphite electrodes (SWCNT-PGEs) developed for DNA hybridization and DNA-drug interactions [40][41][42].…”

“…In aspects of the application of HBV DNA probe immobilized GRPox modified electrode, these single-use sensors have shown very high selectivity and specificity to its complementary DNA sequences even in the mixture sample containing target and a sequence of single base point mutation, when the results were compared to the earlier results [41,[44][45][46][47]. In addition, the preparation of DNA modified GRPox based electrode results with much easier procedure in a shorter time (resulting in approximately 4 hours) contrast to the ones in earlier reports [45][46][47].…”

Estudo eletroquímico do processo de eletrooxidação da amitriptilina empregando sensor a base de pasta de carbono contendo DNA imobilizado em matriz inorgânica

“…Esses picos podem ser atribuídos à oxidação de resíduos das bandas nitrogenadas guanina e adenina do DNA, respectivamente (BRETT at al., 1998;ZHENG et al, 2009;CALISKAN et al, 2009;WANG et al, 2001). Quando comparado ao voltamograma cíclico obtido com eletrodo de trabalho preparado a base de GRF-SiAlNb (ausência de DNA), foi possível observar que nesse caso não ocorreu nenhum processo faradaico no intervalo de potencial estudado, sendo este um resultado esperado.…”

Single‐Walled Carbon Nanotubes Modified Gold Electrodes as an Impedimetric DNA Sensor

“…al. for a carboxylic acid functionalized single-walled carbon nanotubes modified graphite substrate for DNA sensing [35]. The increment on R CT after the attachment of the ssDNA can be attributed to the steric and electrostatic repulsion between the negative charge of the phosphate Fig.…”