Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements

Abstract

Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labeled with horseradish peroxidase. In the presence of H 2 O 2 and methylene blue as the redox mediator, an electrocatalytic current is generated which scales with IgY concentration in the sample. After optimizing the extracting procedure, the immunosensor was applied for analysis of fresh eggs and food integrators. The data obtained with the biosensor were validated by SDS-PAGE and Western blot measurements.

Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken

“…Yolk immunoglobulin (IgY) concentration is often associated as a general indicator of disease resistance. IgY concentrations in blood serum range from 5-15 mg mL -1 (Gaetani et al, 2017). Based on Table 3, the average IgY concentration of IPB-D1 chickens at 21 weeks ranged from 8-11 mg mL -1 .…”

Electrochemical Immunosensors and Aptasensors

“…Moretto, Ugo et al [24] apply ensembles of nanoelectrodes (NEEs), prepared by template Au deposition in nanoporous membranes, to detect, in food integrators, immunoglobulin IgY from hen-eggs yolk. Anti-IgY is immobilized on the polycarbonate templating membrane which surrounds the Au nanoelectrodes, achieving high antibody loadings while keeping unaltered the extremely low detection limits typical of NEEs.…”

Evaluation of Human Anti Igg Polyclonal Antibody Production Conjugated with Peroxidase in Egg Yolk

“…IgG is the most important isotype of immunoglobulin in the secondary immune response. Hens IgG is called IgY (yolk immunoglobulin), which can enter the egg and protect the fetus until it hatches (2,3). Among the medical applications of IgG is the second test for evaluation of patients with symptoms of humoral immune system deficiency or compound immune deficiency (cellular or hormonal) (4).…”

“…Since several years multiple studies have been carried out for using IgGs in medical diagnosis (6,7), and treatment for working against certain types of infectious agents, in particular, intestinal pathogens (8–10). Considering the increasing importance of IgG, researchers have found various methods to produce this type of antibody in hens and to purify them from egg yolk (3, 11, 12). IgG immunoglobulins of one species are immunogenic for other species (13).…”