Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken

Al-Habib, M. F.; Murtini, Sri; Gunawan, Asep; Ulupi, Niken; Sumantri, Cece

doi:10.5398/tasj.2020.43.3.197

Cited by 5 publications

(5 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration of total serum IgY in chickens can be used to measure their fitness, health, and nutrition (Sun et al, 2013). The average IgY concentration of IPB-D2 chickens was higher than IPB-D1 chickens (Al-Habib et al, 2020), and the normal concentration was 5-10 mg mL -1 (Oberlander et al, 2020). Based on statistical analysis, the SNP in the CD4 gene did not significantly differ between genotypes, for IgY concentrations, SNP g.7526 C>T CT has the highest average IgY concentration of 13.85±0.18 mg mL -1 .…”

Section: Discussionmentioning

confidence: 99%

Association and Expression of Cluster of Differentiation 4 (CD4) Gene in IPB-D2 Chicken Related to Immunocompetence Index

Lestari,

Murtini,

Ulupi

et al. 2023

Trop. Anim. Sci. J.

View full text Add to dashboard Cite

The CD4 gene plays an important role in the immune process by producing CD4 molecules that aid in producing antibodies. IPB-D2 chickens are selected from IPB-D2 chickens based on IgY concentration and ND antibody titer. This study aimed to analyze the polymorphism of the CD4 gene, unravel the mRNA expression of CD4 gene in IPB-D2 chicken related to the immunocompetence index, and detect the CD4 gene pathway. The total samples used were 100 IPB-D2 G2 chickens aged 21 weeks. Blood samples were collected for ELISA test, HI test, sequencing test, and seca tonsil tissue for relative mRNA expression. Polymorphism and association data were analyzed using MEGAX, FinchTV, SNPstat, and DNAsp. The relative mRNA expression analysis was conducted using qRT-PCR. The pathway analysis of the CD4 gene was performed using the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analysis. The result showed there were 4 SNPs of the CD4 gene in IPB-D2 chicken, i.e., g.7526 C>T, g.7825 C>T, g.8100 C>A, and g.8157 T>A. All CD4 SNPSs showed no association with IgY concentration and ND antibody titers. Relative mRNA expression shows that IPB-D2 chickens with high ND antibody titers have a higher level of expression when compared to IPB-D2 chickens with low ND antibody titers. Furthermore, pathway analysis showed the CD4 gene involved in the T cell receptor (TcR) signaling process. This study concludes that the CD4 gene is polymorphic and involved in the T cell receptor signaling process. This study demonstrated that polymorphisms of the CD4 gene in IPB-D2 chicken might not contribute to the IgY concentration and ND antibody titer but can serve as a reference in the study of CD4 genes in the other chicken breeds related to the other immunocompetence index.

show abstract

Section: Discussionmentioning

confidence: 99%

Association and Expression of Cluster of Differentiation 4 (CD4) Gene in IPB-D2 Chicken Related to Immunocompetence Index

Lestari,

Murtini,

Ulupi

et al. 2023

Trop. Anim. Sci. J.

View full text Add to dashboard Cite

show abstract

“…Antibody production is induced by exposure to disease through intricate cell signalling (APC, T cells, and B cells). The development of antibodies and the immunological response of chickens can both be affected by communication problems or mistakes (Al-Habib et al 2020).…”

Section: Haplotypementioning

confidence: 99%

“…In the selection program and genetical point of view, the concentration of IgY and ND antibody titers can be employed as indicators. Several genes regulate the synthesis of IgY and ND antibodies (Al-Habib et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of the Thy1 (Thymocyte Differentiation Antigen 1) Gene in Candidate IPB D2 Chicken Line

Putri,

Murtini,

Ulupi

et al. 2022

JITV

View full text Add to dashboard Cite

The Thy1 gene is a member of the immunoglobulin superfamily, which is found in many different animals. This gene encodes a Thy1 protein (CD90) which measures 25â€“37 kDa. Although the function of the Thy1 gene is unknown, it is thought to play a crucial role in the immunological response, particularly in the activation of T cells. The goal of this study was to find a variation in the Thy1 gene in IPB D2 Â chickens that might be utilized as candidates for immunoresistance genetic markers. A total of 123 samples from IPB D2 chickens consisting of 38 samples from IPB D2 G0 (basic generation) chickens and 85 samples from IPB D2 G2 (second generation) chickens were used in this study. PCR methods and direct-DNA sequencing were used in this study. Data analysis in this study consisted of genotype frequency, allele frequency, observed heterozigosity, expected heterozygosity, and Hardy-Weinberg equilibrium. The results of the studyÂ discovered two SNPs on the intron (g.2139 CT and g.2542 AG). Four haplotypes were created by combining two SNPs. The g.2139 CTand g.2542 AG were polymorphic, but not in the Hardy-Weinberg equilibrium.

show abstract

“…IPB-D1 chickens reached 1.38 ± 0.09 kg (male) or 1.18 ± 0.12 kg (female) at the age of 12 weeks (Al-Habib et al 2020a). There have been several studies to improve genetic quality in IPB chickens, such as the prolactin gene in IPB-D1 chickens as a marker for egg production traits (Romlah et al 2020) and CD1B gene diversity for disease resistance traits (Al-Habib et al 2020b).…”

Section: Introductionmentioning

confidence: 99%

Genetic Association and Expression of Myoglobin Gene Related to Mineral Content in IPB-D2 Chickens

P. Liong,

A. K. Alfiah,

I. Khaerunnisa

et al. 2024

JIPTHP

View full text Add to dashboard Cite

Myoglobin (MB) gene encodes the protein of myoglobin, which is a protein found in muscle tissues that plays a crucial role in binding and storing oxygen. This study aimed to analyze the polymorphism of the MB gene, examine its relationship with the meat mineral content, and analyze its expression in the liver and thigh muscle tissue of IPB-D2 chicken. A total of 55 IPB-D2 chickens were used in this study. Identification of the gene polymorphism, quantification of the mineral content, and gene expression were performed using the direct sequencing method, the Atomic Absorption Spectroscopy (AAS) method, and the qRT-PCR, respectively. Chicken thigh muscle and liver tissues were used as the source of mRNA in this study. The statistical analysis methods used were the chi-square test to test Hardy-Weinberg equilibrium, and the T-test for mineral association analysis and gene expression analysis. IPB-D2 chicken on average contains 17.81, 0.22, 16.50, and 0.34 mg/kg of Fe, Se, Zn, and Mn, respectively. Two SNPs were found in 5’UTR of the MB gene, namely SNP g.17 G>T and SNP g.25 T>C. Genotype TT (g.17 G>T) and genotype CC (g.25 T>C) were significantly (P<0.05) associated with high Fe content in IPB-D2 chicken. The gene expression analysis showed that MB mRNA expression in the liver was not statistically different between high Fe (TT/CC) and low Fe (GG/TT and GT/TC) genotype combination. Meanwhile, in the thigh muscle, MB mRNA expression in TT/CC genotype combination was significantly higher than GG/TT and GT/TC genotype combination (P<0.05). These results suggest that the identified polymorphisms in the IPB-D2 MB gene could serve as references for investigating similar gene in other chicken breeds, especially regarding Fe, Se, Zn, and Mn mineral content.

show abstract

Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken

Cited by 5 publications

References 35 publications

Association and Expression of Cluster of Differentiation 4 (CD4) Gene in IPB-D2 Chicken Related to Immunocompetence Index

Association and Expression of Cluster of Differentiation 4 (CD4) Gene in IPB-D2 Chicken Related to Immunocompetence Index

Polymorphisms of the Thy1 (Thymocyte Differentiation Antigen 1) Gene in Candidate IPB D2 Chicken Line

Genetic Association and Expression of Myoglobin Gene Related to Mineral Content in IPB-D2 Chickens

Contact Info

Product

Resources

About