Electrochemical chip-based genomagnetic assay for detection of high-risk human papillomavirus DNA

Bartošík, Martin; Durikova, Helena; Vojtěšek, Bořivoj; Anton, Milan; Jandáková, Eva; Hrstka, Roman

doi:10.1016/j.bios.2016.04.035

Cited by 42 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some of the experimental conditions: the dilution of AbS9.6 (1 : 250 in PBS), temperature of reactions (37 °C), ratio of hydroquinone/hydrogen peroxide (10 mM/50 mM), and applied potential (−0.3 V/60 s) for the chronoamperometric detection, were optimized in our previous works . With the aim of developing a methodology that allows the sensitive determination of the target miRNA with a short and simple working protocol, the number of incubation steps was carefully evaluated.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the efficient incorporation of the resulting magnetic bioconjugates on the surface of planar disposable electrodes in a stable and reproducible way by simple magnetic attraction also implies that detectable products, usually generated through an enzyme reaction, are formed very close to the electrode surface, thus allowing the electrochemical response to be obtained rapidly . In addition, it is important to remark that although the coupling of MBs with single or dual disposable electrodes has been widely described, their use with a screen‐printed carbon electrode array of eight electrodes has been reported only recently by our group . Three miRNAs, miR‐21, let‐7a and miR‐31, associated with development and progression of cervical cancer in women , were selected as target miRNAs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiplexed Immunosensing Platform Coupled to Hybridization Chain Reaction for Electrochemical Determination of MicroRNAs in Clinical Samples

et al. 2018

Self Cite

View full text Add to dashboard Cite

There is an urgent need for development of rapid and inexpensive techniques for detection of microRNAs (miRNAs), which are potential biomarkers of various types of cancer. In this paper, we describe a multiplexed electrochemical platform for determination of three cancer‐relevant miRNAs: miR‐21, let‐7a and miR‐31. The strategy combines the use of magnetic beads (MBs) modified with a commercial antibody for the efficient capture of the heteroduplexes formed by hybridization of the target miRNA with DNA probe. Free non‐hybridized region of the DNA probe was thereafter hybridized with two biotin‐labeled auxiliary DNA probes in a process of hybridization chain reaction (HCR), resulting in a long hybrid bearing a large number of biotin molecules. Labeling of these multiple biotin units with streptavidin‐peroxidase conjugates allowed an amplification of the amperometric signal measured after capturing the modified MBs at a screen‐printed carbon electrode array of eight electrodes. The combined strategy demonstrated in a similar assay time significantly higher sensitivity than those previously described using modified MBs with the same capture antibody (without amplification by HCR) or a HCR strategy implemented on the surface of MBs, respectively. The methodology exhibits a good selectivity for discriminating single mismatches and was applied to the determination of the three target miRNAs in total RNA (RNAt) extracted from various cancer cell lines and from cervical precancerous lesions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiplexed Immunosensing Platform Coupled to Hybridization Chain Reaction for Electrochemical Determination of MicroRNAs in Clinical Samples

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bartosik et al presented a SPE electrode that assembled by the means of streptavidin‐modified magnetic beads and a DNA capture probe to detect HPV16 DNA by using a digoxigenin label. The detection range of this biosensor was 1 pM to around 1 nM .…”

Section: Methodsmentioning

confidence: 99%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

View full text Add to dashboard Cite

Human papillomavirus type (HPV) is a common cause of sexually transmitted disease (STD) in humans. HPV types 16 and 18 as the highest risk types are related with gynecologic malignancy and cervical cancer (CC) among women worldwide. Recently, considerable development of genosensors, which allows dynamic monitoring of hybridization events for HPV‐16 and 18, has been a topic of focus by many researchers. In this systematic review, we highlight the route of development of DNA‐based genosensory detection methods for diagnosis of high risk of HPV precancer. Biosensor detection methods of HPV‐16 and 18 was investigated from 1994 to 2018 using several databases including PubMed, Cochrane Library, Scopus, Google Scholar, SID, and Scientific Information Database. Manual search of references of retrieved articles were also performed. A total of 50 studies were reviewed. By analyzing the most recent developed electrochemical biosensors for the identification of HPV, we observed that the sensor platform fabricated by Wang et al. holds the lowest detection limit reported in the literature for the DNA of HPV‐16. Up to this date, optical, electrochemical, and piezoelectric systems are the main transducers used in the development of biosensors. Among the most sensitive techniques available to study the biorecognition activity of the sensors, we highlight the biosensors based fluorescent, EIS, and QCM. The current systematic review focuses on the sensory diagnostic methods that are being used to detect HPV‐16 and 18 worldwide. Special emphasis is given on the sensory techniques that can diagnosis the individuals with CC. © 2018 BioFactors, 45(2):101–117, 2019

show abstract

“…Objevilo se i několik strategií pro stanovení přítom-nosti DNA pocházející z HPV 16 [25,26], ovšem ne všechny práce demonstrovaly použitelnost metody na klinických vzorcích. Nedávno byl vyvinut rychlý elektrochemický test pro detekci HPV 16 aplikovatelný i na klinickém materiálu [27]. Pomocí magnetických kuliček byla ze vzorků DNA (izolovaných z kartáčko-vých stěrů) separována virová DNA z HPV 16 a po PCR amplifikaci byla sledována elektrochemická odezva na elektrodových čipech, které umožňují paralelní detekci více vzorků (obr.…”

Section: Závěr a Výhled Do Budoucnostiunclassified

Human Papillomavirus – Role in Cervical Carcinogenesis and Methods of Detection

Bartošík¹,

Hrstka²,

Jiráková³

2018

Klin Onkol

Self Cite

View full text Add to dashboard Cite

Východiska: Perzistentní infekce onkogenním typem lidského papilomaviru (human papillomavirus -HPV), nejčastěji HPV 16 a HPV 18, může vést k rozvoji řady maligních onemocnění, zejména pak ke karcinomu děložního hrdla u žen. Testování na přítomnost HPV DNA se tudíž zavádí jako komplementární metoda ke stávajícímu cytologickému vyšetření, mimo jiné i díky vyšší citlivosti. Cíl: Tento přehledový článek pojednává o roli HPV v karcinogenezi děložního hrdla, zejména popisuje vznik cervikálních intraepiteliálních lézí (CIN1-3) a molekulární mechanizmus transformace buněk. Představuje existující bio markery pro dia gnostiku prekancerózních lézí, mezi které patří např. mRNA transkripty genů E6 a E7, protein p16 (inhibitor cyklin-dependentní kinázy regulující přechod z G1 do S fáze buněčného cyklu), změněné metylační vzorce DNA nebo působení specifických miRNA, tj. krátkých nekódujících jednořetězcových RNA o délce 18-22 nukleotidů, které posttranskripčně regulují genovou expresi. Dále rozebírá výhody a nevýhody komerčně dostupných HPV testů, a v neposlední řadě představuje nově vyvíjené metody umožňující levnou a rychlou dia gnostiku HPV na bázi optické i elektrochemické detekce. Závěr: Nádory děložního hrdla i navzdory velkému pokroku v posledních letech stále patří mezi nejrozšířenější ženské malignity s vysokou mortalitou, zejména v rozvojových zemích. Ke snížení mortality by mohla pomoci implementace HPV testování do rutinního screeningového vyšetření. To však bude třeba finančně zefektivnit tak, aby bylo cenově konkurenceschopné se stávajícími cytologickými vyšetřeními. Klíčová slovaHPV -karcinom cervixu -HPV testování -hybridizace nukleových kyselin -mRNA -metylace DNA -miRNA SummaryBackground: Persistent infection with high-risk human papillomavirus (HPV) strains, especially HPV 16 and HPV 18, is associated with the onset of various malignant dis eases, includ ing cervical carcinoma in women. HPV DNA test ing is thus be ing implemented as a complementary method to standard cytological examination, mainly due to its increased sensitivity. Aim: This review outlines the role of HPV in cervical carcinogenesis, with a focus on the formation of cervical intraepithelial neoplasias (CIN1-3) and the molecular mechanism underly ing cellular transformation. Current bio markers used to screen premalignant lesions are described, includ ing mRNA transcripts of the E6 and E7 genes, protein p16 (a cyclin-dependent kinase inhibitor that regulates cell cycle progression from G1 to S phase), altered DNA methylation patterns, and actions of specific microRNAs (short (18-22 bp), non-coding, single-stranded RNA molecules that regulate gene expression at the post-transcriptional level). This review also describes the advantages and drawbacks of commercial HPV tests, and depicts novel methods for more cost-effective and faster HPV dia gnostics based on optical or electrochemical detection. Conclusion: Although great progress has been made, the incidence and mortality rates of cervical malignancies remain relatively high , especially in d...

show abstract

Electrochemical chip-based genomagnetic assay for detection of high-risk human papillomavirus DNA

Cited by 42 publications

References 26 publications

Multiplexed Immunosensing Platform Coupled to Hybridization Chain Reaction for Electrochemical Determination of MicroRNAs in Clinical Samples

Multiplexed Immunosensing Platform Coupled to Hybridization Chain Reaction for Electrochemical Determination of MicroRNAs in Clinical Samples

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

Human Papillomavirus – Role in Cervical Carcinogenesis and Methods of Detection

Contact Info

Product

Resources

About