EKSPLORASI BAKTERI FILOSFER DARI TANAMAN MANGROVE SEBAGAI BAKTERI PROBIOTIK PADA BUDI DAYA UDANG WINDU, Penaeus monodon

Muliani, Muliani; Nurbaya, Nurbaya; Tompo, Arifuddin; Armomarsono, Muharijadi

doi:10.15578/jppi.10.2.2004.47-57

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…Samples of C. esculenta L were collected from Percut Sei Tuan District, Medan City, North Sumatra (3°35'07.0"N, 98°44'10.9"E). Isolation of phylloplane bacteria was based on the method by Muliani et al (2004). Briefly, the leaf sample was cut into smaller size, approximately 2-to-5-cm fragments then placed on top of Nutrient Agar (NA) medium, each for the upper and lower surface of leaves.…”

Section: Isolation Of Phylloplane Bacteriamentioning

confidence: 99%

Biosurfactant activity of phylloplane bacteria from an ornamental plant, Colocasia esculenta L.

2022

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Abstract. Bukhori A, Suryanto D, Nurtjahja K. 2022. Biosurfactant activity of phylloplane bacteria from an ornamental plant, Colocasia esculenta L. Biodiversitas 23: 3108-3114. Biosurfactants are surface-active molecules produced by living organisms predominantly by microorganisms with amphiphilic properties. Exploration of biosurfactant-producing bacteria has been promoted to find the suitable agent for mass production in the laboratory following its biochemical and genetic modification. Leaf-colonizing bacteria or phyllosphere bacteria are of great interest, including those colonizing the surface of ornamental plants which are still understudied. This study aimed to isolate the biosurfactant-producing bacteria from an ornamental plant, Colocasia esculenta L and to determine their physical characteristics. Four bacterial isolates coded as IC1, IC3, IC4 and IC5 were recovered from the surface of C. esculenta and were tested positive for their growth under Bushnell-Haas agar + 1% olive oil (v/v) as the sole carbon source. Two isolates, namely IC3 and IC5, later molecularly identified as Bacillus cereus and Alcaligenes faecalis produced the highest biosurfactant concentration (IC3 = 157 ppm, IC5 = 106 ppm) on 10th day incubation based on a colorimetric test using rhamnolipid as a standard solution. Crude biosurfactants produced by A. faecalis IC5 showed better physical attributes than B. cereus IC3 in terms of surface tension, emulsification index, and oil spreading capability on four different hydrophobic compounds i.e., kerosene, solar fuel, octane fuel (Pertalite, Pertamax). The results of this study confirmed the existence of biosurfactant-producing bacteria in C. esculenta and the possibility of developing prominent strains for the treatment of hydrocarbon pollution in the environment.

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Section: Isolation Of Phylloplane Bacteriamentioning

confidence: 99%

Biosurfactant activity of phylloplane bacteria from an ornamental plant, Colocasia esculenta L.

2022

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“…Hal ini juga memacu kegiatan ekplorasi bakteri-bakteri alam dari berbagai sumber yang potensial digunakan sebagai probiotik dan biokontrol. Beberapa sumber bakteri probiotik yang telah dikaji antara lain air laut dan sedimen (Muliani et al, 2003;Tjahjadi et al, 1994), koral (Radjasa et al, 2005), hatcheri (Rosa et al, 1997;Hala 1999;Haryanti et al, 2000), daun mangrove (Muliani et al, 2004), dan tambak udang (Muliani et al, 2006), dari seabream (Sparus aurata, L.) (Chabrillon et al, 2006), dari usus ikan-ikan lele (Sugita et al, 2007), dari ikan nila (Aly et al, 2008a), dari usus ikan (Balcazar et al, 2008).…”

Section: Pendahuluanunclassified

PENGARUH PERBEDAAN WAKTU APLIKASI PROBIOTIK TERHADAP KUALITAS AIR DAN SINTASAN PASCA LARVA UDANG WINDU (Penaeus monodon)

Muliani

Nurbaya

Atmomarsono

2016

Jurnal Riset Akuakultur

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Penelitian ditujukan untuk mengetahui pengaruh waktu pemberian probiotik yang berbeda terhadap perubahan kualitas air dan sintasan udang windu dalam skala laboratorium. Penelitian dilaksanakan di Laboratorium Basah Balai Riset Perikanan Budidaya Air Payau (BRPBAP), Maros menggunakan 21 buah akuarium berukuran 40 cm x 30 cm x 27 cm yang diisi tanah tambak setebal 10 cm dan air laut salinitas 28 ppt sebanyak 15 L serta 30 ekor pascalarva udang windu. Probiotik yang digunakan adalah kombinasi BL542+BT951+MY1112 dengan perlakuan; (A) aplikasi probiotik pada awal sampai akhir penelitian; (B) aplikasi probiotik pada minggu ke-II sampai akhir penelitian; (C) aplikasi probiotik pada minggu ke-IV sampai akhir penelitian; (D) aplikasi probiotik pada minggu ke-VI sampai akhir penelitian; (E) aplikasi probiotik pada minggu ke-VIII sampai akhir penelitian; (F) aplikasi probiotik pada minggu ke X sampai akhir penelitian; (G) kontrol (tanpa probiotik), masing-masing diulang 3 kali. Penelitian berlangsung selama 12 minggu. Pengamatan parameter kualitas air dilakukan setiap 2 minggu yang meliputi; total bakteri, total Vibrio spp., BOT, NH3-N, NO2-N, NO3-N, PO4-P. Sedangkan pengamatan sintasan udang windu dilakukan pada akhir penelitian. Hasil penelitian menunjukkan bahwa aplikasi probiotik pada minggu ke-IV dapat menekan konsentrasi BOT dan NH3-N, menurunkan total Vibrio sp. sehingga berdampak kepada peningkatan sintasan udang windu

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“…Samosir, Suryanto, and Desrita (2017) established one by developing probiotic from the surrounding ecosystem. Many isolated bacteria with potential sources of probiotic can be collected from mangrove communities, such as from mangrove plant (its root and bark), fishes like milky fish, cuttlefish, mangrove shrimps, molluscs, crabs, and other fishes, as well as from the mud, water and other abiotic factors of the mangrove ecosystem (Pratiwi, Rahayu, & Wahju, 2013;Seprianto, Feliatra, & Nugroho, 2017;Muliani, Nurbaya, Arifudin, & Muharijadi, 2017). Therefore, it is necessary to figure out any potential of the proteolytic Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

PROBIOTIC CANDIDATE PROTEOLYTIC Bacillus sp. COLLECTED FROM MANGROVE OF MARGASARI, LAMPUNG

et al. 2021

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Intensive shrimp culture has encountered many problems, such as declining water quality through disease caused by pathogenic microbes, which affected mortality. This study aimed to determine any potential probiotic from Bacillus sp. collected from mangrove in East Lampung, which could be used to improve the cultured shrimps' proteolytic and probiotic activity. This is a descriptive research with sampling and data collection of bacteria from many samples of mangrove. Result shows 128 isolates Bacillus from which then it has arrived at five potential probiotic Bacillus sp. The study five Bacillus sp. has been isolated with potential properties for probiotic (KPP212, IP121, UJ131, UJ132, SB141). Each isolate has characteristics with proteolytic property, growth in a wide range of pH 4–10 and osmotic stress (0–6% NaCl), non-pathogenic, ability for glucose fermentation, non-motile, and has negative catalase activity. The five potential Bacillus sp. can be used as probiotics for shrimp farming.

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EKSPLORASI BAKTERI FILOSFER DARI TANAMAN MANGROVE SEBAGAI BAKTERI PROBIOTIK PADA BUDI DAYA UDANG WINDU, Penaeus monodon

Cited by 5 publications

References 6 publications

Biosurfactant activity of phylloplane bacteria from an ornamental plant, Colocasia esculenta L.

Biosurfactant activity of phylloplane bacteria from an ornamental plant, Colocasia esculenta L.

PENGARUH PERBEDAAN WAKTU APLIKASI PROBIOTIK TERHADAP KUALITAS AIR DAN SINTASAN PASCA LARVA UDANG WINDU (Penaeus monodon)

PROBIOTIC CANDIDATE PROTEOLYTIC Bacillus sp. COLLECTED FROM MANGROVE OF MARGASARI, LAMPUNG

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