Efficient shoot regeneration from direct apical meristem tissue to produce virus-free purple passion fruit plants

Prammanee, S.; Thumjamras, S.; Chiemsombat, P.; Pipattanawong, Narongchai

doi:10.1016/j.cropro.2011.07.008

Cited by 28 publications

(17 citation statements)

References 12 publications

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“…No gênero Passiflora, plantas livres de vírus podem ser obtidas in vitro a partir de meristema apical (PRAMMANEE et al, 2011), e também a técnica da enxertia tem sido aprimorada, como, por exemplo, através do uso da minienxertia, o que visa à obtenção de materiais livres de vírus, como o Cowpea aphid-borne mosaic virus (CABMV) (RIBEIRO et al, 2008). Segundo Maciel et al (2009), plantas de P. suberosa enxertadas em plantas de P. edulis f. flavicarpa infectadas com o CABMV não desenvolveram sintomas da doença.…”

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Metodologia de minienxertia em maracujazeiros

Alexandre

Lopes

Tiradentes³

et al. 2013

Rev. Bras. Frutic.

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O objetivo foi avaliar a metodologia de enxertia pela modalidade garfagem no topo hipocotiledonar em fenda cheia, utilizando miniestacas adultas de segmentos apicais de P. edulis f. flavicarpa, P. edulis, P. alata, P. mucronata e P. foetida e internodais de P. mucronata. O experimento foi conduzido em casa de vegetação equipada com sistema de irrigação com nebulização intermitente, do Centro Universitário Norte do Espírito Santo (CEUNES), da Universidade Federal do Espírito Santo (UFES). Os índices de pegamento nas combinações de P. edulis f. flavicarpa/P. edulis f. flavicarpa, P. edulis/P. edulis f. flavicarpa, P. alata/P. edulis f. flavicarpa, P. mucronata/P. edulis f. flavicarpa, P. foetida/P. edulis f. flavicarpa foram de 90; 90; 68; 80 e 90, respectivamente. A metodologia empregada é uma nova opção de enxertia para essas espécies do gênero Passiflora.

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Metodologia de minienxertia em maracujazeiros

Alexandre

Lopes

Tiradentes³

et al. 2013

Rev. Bras. Frutic.

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“…In vitro propagation is a promising approach for generating aseptic seedlings and for reducing or overcoming dormancy, in addition to other possibilities, such as obtaining virus-free seedlings (Prammanee et al, 2011) and producing secondary metabolites (Passos et al, 2004;Rosa and Dornelas, 2012). Complete removal of the seed integument is an essential step for the in vitro germination of Passiflora edulis f. flavicarpa (Dias et al, 2009;Rêgo et al, 2011), P. cincinnata (Dias et al, 2009;Dias et al, 2010), P. foetida (Rosa and Dornelas, 2012), and P. foetida var.…”

Section: Discussionmentioning

confidence: 99%

Morphometry, in vitro-ex vitro germination and tetrazolium testing of stinking passionflower [Passiflora foetida var. glaziovii Killip] (Passifloraceae) seeds

Costa¹,

Oliveira²,

Araújo³

et al. 2016

Aust J Crop Sci

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Passiflora foetida L. has broad utility as a medicinal and ornamental plant; however, information regarding varieties and the physiological potential of their seeds are scarce. Here, we aim to evaluate in vitro and ex vitro germination behaviour and to adapt tetrazolium methodology for P. foetida var. glaziovii Killip. The botanical identification and characterisation were performed through the observation of morphological characters and consultation of the relevant literature. Ex vitro germination was performed on rolled and flat Germitest paper at continuous (25 °C) and alternating (20 and 30 °C; night and day, respectively) temperatures. Seeds with the completely removed integument were used for in vitro germination and tetrazolium tests. The in vitro germination test was performed with MS medium using seeds conditioned under different light qualities (white, red, far-red or darkness) and at two temperature regimes (25 °C; 20 and 30 °C alternating temperature). For the tetrazolium test, seeds were soaked in different concentrations of tetrazolium salt (2.5, 5.0, 7.5 or 10 g L -1 ) at different temperatures (30, 35, 40 or 45 °C) for two hours in the dark. This is the first record of P. foetida var. glaziovii, a wild herbaceous vine with glandular trichomes on its leaves, bracts and stipules; fruit bacoid, elliptic, glabrous, in Espírito Santo State, Brazil. The seeds are small (approximately 4.87 mm long and 2.15 mm wide) and non-photoblastic, and in vitro germination must be performed using an alternating temperature (20 and 30 °C) regime. The best result of germination was achieved using a concentration-temperature combination of 10 g L -1 tetrazolium and 30 °C.

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“…The opposite behavior was expected because the plants from AGBP accession 158 were 3 months old and those from AGBP accession 38 were 1 year and 8 months old, and younger donor plants have been shown to exhibit higher organogenic ability. However, Prammanee et al (2011) obtained plants regenerated from explants of 0.2 cm P. edulis (purple passion). On the other hand Gómez & González (2010) reported in vitro shoot formation from axillary bud of adult P. edulis, but there was no indication of plant regeneration.…”

Section: Resultsmentioning

confidence: 99%

In vitro culture of shoot apices from juvenile and adult yellow passion fruit plants

Junghans

Andrade

Simões

et al. 2014

Agraria

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The aim of the present study was to evaluate in vitro plant regeneration from the shoot apices of juvenile and adult passion fruit plants and provide a basis for future clonal cleanup of superior genotypes through the use of tissue culture. The studies were divided into three experiments to evaluate the effect of age and origin of the donor plant, the culture medium methodology and the size of the explants. The shoot apices were inoculated in Murashige & Skoog (MS) medium with 3% sucrose and three 6-benzylaminopurine (BAP) concentrations: 0, 1 or 2 mg L -1 . Next, the samples were transferred to a half-strength mineral salt medium (½ MS) and supplemented with 1 mg L -1 indolebutyric acid (IBA). Two cultivation methods were used: one with variations in the cultivation period in the MS medium (26 or 60 days) and the other with or without transfer to half-strength mineral salt medium (½ MS). The methodology by which explants are maintained in MS medium supplemented with 1 mg L -1 BAP for 26 days, followed by transfer to ½ MS medium supplemented with 1 mg L -1 IBA for 21 days of cultivation and then transfer to ½ MS medium for 14 days is more appropriate for regenerating plants from the shoot apices of adult Passiflora edulis (AGBP accession 38).Key words: growth regulators, Passiflora edulis, tissue culture Cultivo in vitro de ápices caulinares provenientes de plantas jovens e adultas de maracujazeiro amarelo RESUMO O objetivo deste trabalho foi avaliar a regeneração de plantas in vitro a partir de ápices caulinares provenientes de maracujazeiros jovens e adultos e fornecer subsídios para futuros trabalhos de limpeza clonal de genótipos superiores, via cultura de tecidos. Os estudos foram divididos em três experimentos visando avaliar o efeito da idade e a procedência da planta doadora, da metodologia do meio de cultivo e do tamanho dos explantes. Os ápices caulinares foram inoculados em meio de estabelecimento Murashige & Skoog (MS), acrescido de 3% de sacarose e com três concentrações de BAP: 0; 1 mg L -1 ou 2 mg L -1 . Em seguida, foram transferidos para o meio com metade das concentrações dos sais minerais (½MS) acrescida de 1 mg L -1 de AIB. Duas formas de cultivo foram utilizadas variando o período de cultivo no meio de estabelecimento (60 dias ou 26 dias) e a transferência ou não para o meio, com metade das concentrações dos sais minerais (½MS). A metodologia na qual os explantes são mantidos em meio MS acrescido de 1 mg L -1 de BAP durante 26 dias seguidos de transferência para o meio ½MS acrescido de 1 mg L -1 de AIB, por 21 dias de cultivo e, posteriormente, para o meio ½MS, por 14 dias, é mais apropriada para regenerar plantas a partir de ápices caulinares de plantas adultas de Passiflora edulis (acesso BGP 38).Palavras-chave: reguladores de crescimento, Passiflora edulis, cultura de tecidos

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Efficient shoot regeneration from direct apical meristem tissue to produce virus-free purple passion fruit plants

Cited by 28 publications

References 12 publications

Metodologia de minienxertia em maracujazeiros

Metodologia de minienxertia em maracujazeiros

Morphometry, in vitro-ex vitro germination and tetrazolium testing of stinking passionflower [Passiflora foetida var. glaziovii Killip] (Passifloraceae) seeds

In vitro culture of shoot apices from juvenile and adult yellow passion fruit plants

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