2008
DOI: 10.1080/10496470802598842
|View full text |Cite
|
Sign up to set email alerts
|

EfficientIn-VitroRegeneration from Mature Leaf Explants ofScoparia dulcisL., an Ethnomedicinal Plant

Abstract: A plant regeneration protocol via multiple-shoot induction using leaf explants from field-grown mature plants of Scoparia dulcis L. (Scrophulariaceae), an ethnomedicinal plant, was established. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP, 13.2, 17.6 and 22.2 μM) in combination with indole-3-acetic acid (IAA, 0.5 and 2.8 μM) or napthalene-3-acetic acid (NAA, 0.5 and 2.6 μM). The maximum number of shoots (14.0 ± 1.14) with longest shoot leng… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
13
0

Year Published

2011
2011
2020
2020

Publication Types

Select...
6

Relationship

1
5

Authors

Journals

citations
Cited by 9 publications
(13 citation statements)
references
References 10 publications
0
13
0
Order By: Relevance
“…Different from our previous regeneration studies of S. dulcis in which we had used basal medium for elongation of shoots after shoot induction (Aileni et al 2008), in the present report the transformed shoot primordia were transferred onto the medium with lower concentration of BAP for promoting shoot elongation (Fig. 1g).…”
Section: Discussionmentioning
confidence: 96%
See 2 more Smart Citations
“…Different from our previous regeneration studies of S. dulcis in which we had used basal medium for elongation of shoots after shoot induction (Aileni et al 2008), in the present report the transformed shoot primordia were transferred onto the medium with lower concentration of BAP for promoting shoot elongation (Fig. 1g).…”
Section: Discussionmentioning
confidence: 96%
“…Based on our prior report in regenerating 14 ± 1.14 plantlets per mature leaf explant (Aileni et al 2008), in the present investigation we have also used the optimal combination and concentrations of plant growth regulators for regeneration of shoot primordia after cocultivation. Different from our previous regeneration studies of S. dulcis in which we had used basal medium for elongation of shoots after shoot induction (Aileni et al 2008), in the present report the transformed shoot primordia were transferred onto the medium with lower concentration of BAP for promoting shoot elongation (Fig.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Although in vitro propagation of S. dulcis with leaf explants [14] and with nodal segment [13] were reported, in this report, the total time schedule was compressed as well as produced 59 shoots from a single explants. About three fold increased in direct shoot formation comparing with the previous report.…”
Section: Discussionmentioning
confidence: 99%
“…Traditionally the plant is used to treat kidney stone and urinary troubles [6][7][8][9][10][11][12] . Though micropropagation has been performed in S. dulcis [13,14] further studies on factors that may enhance the process are needed. The objective of the present study was to develop an improved protocol for micropropagation of S. dulcis.…”
Section: Introductionmentioning
confidence: 99%