2011
DOI: 10.1007/s11816-011-0166-3
|View full text |Cite
|
Sign up to set email alerts
|

Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis

Abstract: Efficient Agrobacterium-mediated genetic transformation of Scoparia dulcis L. was developed using Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pCAMBIA1301 with b-glucuronidase (GUS) (uidA) and hygromycin phosphotransferase (hpt) genes. Two-day precultured leaf segments of in vitro shoot culture were found to be suitable for cocultivation with the Agrobacterium strain, and acetosyringone was able to promote the transformation process. After selection on shoot organogenesis medium with ap… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

1
10
1

Year Published

2012
2012
2019
2019

Publication Types

Select...
6

Relationship

1
5

Authors

Journals

citations
Cited by 13 publications
(12 citation statements)
references
References 32 publications
(35 reference statements)
1
10
1
Order By: Relevance
“…In conclusion, we for the first time established a simple and efficient protocol for recovery of transgenic S. dulcis generated via biolistic-mediated nuclear transformation. Although the stable transformation efficiency (52.0 %) reported in the present study is less than that of earlier report (54.6 %) that which was attained by Agrobacterium mediated transformation (Aileni et al 2011a). However, biolistic method is the most efficient way to adopt towards plastid transformation in S. dulcis.…”
Section: Discussioncontrasting
confidence: 82%
See 2 more Smart Citations
“…In conclusion, we for the first time established a simple and efficient protocol for recovery of transgenic S. dulcis generated via biolistic-mediated nuclear transformation. Although the stable transformation efficiency (52.0 %) reported in the present study is less than that of earlier report (54.6 %) that which was attained by Agrobacterium mediated transformation (Aileni et al 2011a). However, biolistic method is the most efficient way to adopt towards plastid transformation in S. dulcis.…”
Section: Discussioncontrasting
confidence: 82%
“…The availability of biolistic mediated-nuclear transformation in S. dulcis, has significant interest in developing transplastomic plants for metabolic engineering of certain regulatory gene traits and also in extensive development of molecular farming for production of biopharmaceuticals (Bock 2007). In previous transformation studies of S. dulcis 3.9 ± 0.39 transgenic plantlets per leaf explant were recorded (Aileni et al 2011a). While there was no remarkable differences observed in the present investigation (3.7 ± 0.24 transgenic plantlets per leaf/explant).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In our lab, a reliable regeneration, Agrobacterium and biolistic mediated genetic transformation using uidA, hpt and gus and nptII genes respectively in S. dulcis (Aileni et al 2011a;Srinivas et al 2016). In Scoparia dulcis, efficient plant regeneration system comparable to model plant system like tobacco using leaf explant made more convenient to achieve reliable chloroplast transformation by targeting rpl32/trnL and trnR/trnN insertional sites of SSC and IR regions of plastid genome using pFaadAII and KNTc heterologous vectors respectively (Muralikrishna et al 2016;Narra et al 2018a).…”
Section: Introductionmentioning
confidence: 99%
“…Stable genetic transformation in S. dulcis was achevied through Agrobacterium using uidA and hpt genes (Aileni et al 2011) and efficient biolistic transformation using uidA and npt II genes (Srinivas et al 2016). Development of chloroplast transformation in this plant system will provide new opportunities towards plastid based metabolic engineering of certain valuable natural product entities and to delve this technology towards enhanced metabolite production and extension in engineering of biosynthetic pathways by plastid transformation.…”
Section: Introductionmentioning
confidence: 99%