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Psoralea corylifolia is a medically important plant and it used for large scale level in pharmaceutical industries to cure various skin diseases. This plant is facing difficulties in propagation because of poor seed germination and high mortality of seedlings. Therefore, an efficient and simple protocol developed for seed germination of Psoralea corylifolia via hot water treatment for in situ and ex situ plant propagation and conservation. Different treatments such as hot water heat treatment (10°C to 100°C) and sulfuric acid (H 2 So 4 ) treatment (5 to 30 min) were used for seed germination. In which, hot water heat treatment with 70°C was produced the highest seed germination (70%) and survival rate. It is concluded that the hot water heat treatment favorably overcome the dormancy of seed. Developed protocol in this study will be helpful for mass propagation and in situ and ex situ conservations.

Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on halfstrength MS medium supplemented with 2.46 µM·l -1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.

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