Efficiency of Pleurotus florida Laccase on Decolorization and Detoxification of the Reactive Dye Remazol Brilliant Blue R (RBBR) under Optimized Conditions

Abstract

Laccase from the white‐rot fungus Pleurotus florida, produced under solid‐state fermentation conditions, was used for the decolorization of reactive dye Remazol Brilliant Blue R (RBBR). RBBR was decolorized up to 46% by P. florida laccase alone in 10 min. In the presence of N‐hydroxybenzotriazole (HBT), the rate of decolorization was enhanced 1.56‐fold. Central composite design of response surface methodology with four variables namely, dye, enzyme, redox mediator concentrations, and time at five levels was applied to optimize the RBBR decolorization. The predicted optimum level of variables for maximum RBBR decolorization (87%) was found to be 52.90 mg L−1 (RBBR), 1.87 U mL−1 (laccase), 0.85 mM (HBT), and 7.17 min (time), respectively. The validation results showed that the experimental value of RBBR decolorization (82%) was close to the predicted one. The disappearance of C–N and C–X groups, and a small shift in N–H groups in Fourier‐transform infra red (FTIR) spectroscopy confirms the degradation of RBBR chromophore by laccase enzyme. The phytotoxicity of RBBR was considerably reduced after the treatment with laccase. RBBR decolorization kinetics; Km and Vmax were calculated to be 145.82 mg L−1 and 24.86 mg L−1 min, respectively.

Evaluation of Catalytic Efficiency of Coriolopsis caperata DN Laccase to Decolorize and Detoxify RBBR Dye

“…According to Zucconi et al [33], less than 50% GI value indicates high phytotoxicity, values between 50 and 80% mean moderate phytotoxicity, and more than 80% indicates no phytotoxicity. Thus, the toxicity of metabolites produced after the treatment of laccase from C. caperata DN to RBBR dye is much lower than that of RBBR dye, and similar result was reported by Osma et al [24] and Sathishkumar et al [11].…”

“…Ligninolytic white rot fungi effectively degrade recalcitrant synthetic dyes due to the production of extracellular ligninolytic enzymes, mainly laccases [11,[18][19][20]. In the present study, we used laccase enzyme produced by indigenous fungal isolate C. caperata DN, under submerged condition, to decolorize and detoxify anthraquinone dye RBBR.…”

“…The number of RBBR molecules turned over per laccase molecule (K cat value) was 135.0 S , indicating high catalytic power of enzyme. Sathishkumar et al [11] reported that the K m and V max values of P. florida laccase for RBBR dye decolorization was 145.82 mg l −1 and 24.86 mg l…”

“…For making the overall process cost effective and time saving, statistical approaches like response surface methodology (RSM) can be applied to optimize enzymatic dye decolorization process. Recently, the potential application of RSM in the laccase-mediated decolorization of various dyes has been published [11].…”

Cross-linked enzyme aggregates of Cerrena laccase: Preparation, enhanced NaCl tolerance and decolorization of Remazol Brilliant Blue Reactive

“…RBBR is a recalcitrant anthraquinone dye frequently used as a starting material in production of polymeric dyes [26,27] . Free and immobilized Cerrena laccases showed similar RBBR decolorization ability; 2 U/mL laccase decolorized 100 mg/L RBBR by > 90% in 40 min without the help of a redox mediator ( Fig.…”