SUMMARY
5-methylcytosine is a major epigenetic modification that is sometimes called “the fifth nucleotide.” However, our knowledge of how offspring inherit the DNA methylome from parents is limited. We generated nine single-base resolution DNA methylomes, including zebrafish gametes and early embryos. The oocyte methylome is significantly hypomethylated compared to sperm. Strikingly, the paternal DNA methylation pattern is maintained throughout early embryogenesis. The maternal DNA methylation pattern is maintained until the 16-cell stage. Then, the oocyte methylome is gradually discarded through cell division and is progressively reprogrammed to a pattern similar to that of the sperm methylome. The passive demethylation rate and the de novo methylation rate are similar in the maternal DNA. By the midblastula stage, the embryo’s methylome is virtually identical to the sperm methylome. Moreover, inheritance of the sperm methylome facilitates the epigenetic regulation of embryogenesis. Therefore, besides DNA sequences, sperm DNA methylome is also inherited in zebrafish early embryos.
Electroadhesion generates an adhesion force using an externally applied power source, which has versatile applications in robotics and material handling. In this study, a self-powered electroadhesion system using enhanced triboelectric nanogenerators (TENGs) to supply power for electroadhesion is presented. By introducing a triboelectric charge supplement channel, the open circuit voltage of the TENG can be significantly boosted by over 10 times, from ∼230 V to more than 3300 V for a single TENG unit, providing sufficiently high voltage for an electroadhesive patch to generate enough adhesion for practical use. The charge supplement channel takes effect through a replenishing mechanism for dissipated charges, maintaining an optimal charge distribution throughout TENG electrodes, which enables the highest open circuit voltage under given surface charge density and device configuration. The fabricated self-powered electroadhesion system shows the ability to manipulate objects of various materials via easy and straightforward operations, demonstrating a great potential for applications in material handling and robotics. Moreover, the voltage enhancement mechanism by the charge supplement channel could be extended to TENGs of other modes, which can provide reliable power sources for various applications that require a high voltage.
In this paper, simple small molecules, glyoxal and acrylonitrile, are chosen as starting materials to prepare an amidoxime-functionalized hydrothermal carbon-based solid phase extractant (HTC-AO) via a one-step hydrothermal process following a simple oximation. The resulting HTC-AO exhibits the anticipated properties, i.e., low porosity (0.01 cm 3 g À1 ) and intraparticle diffusion coefficient (k int ¼ 0.042 mmol g À1 min À0.5 ), high content of amidoxime groups (1.66 mmol g À1 ) and minimal undesired functional groups (typically carboxylic group: 0.07 mmol g À1 ; phenolic group: 0.38 mmol g À1 ; lactonic group: 0.01 mmol g À1 ). Moreover, the results of irradiation experiments under g-ray dosages between 1 and 100 kGy indicate that HTC-AO has good radiation stability. The sorption behavior of U(VI) onto HTC-AO is investigated in detail using batch sorption experiments. A saturation U(VI) sorption capacity over that of all the uranium sorbents reported previously is found to be 1021.6 mg g À1 at pH 4.5 in single uranium solution, and a so far unreported highest uranium selectivity of 81.6% with a sorption capacity of 268.9 mg g À1 is observed at pH 2.5 in multi-ion solution. The significant outcomes in this work confirms that the "simple small molecule carbon source" strategy is practical and efficient, and may have the potential for the preparation of other types of functional materials such as highly specific catalysts, drug targeting carriers and others.
Malachite green (MG) was decolorized by laccase (LacA) of white-rot fungus Cerrena sp. with strong decolorizing ability. Decolorization conditions were optimized with response surface methodology. A highly significant quadratic model was developed to investigate MG decolorization with LacA, and the maximum MG decolorization ratio of 91.6% was predicted under the conditions of 2.8 U mL-1 LacA, 109.9 mg L-1 MG and decolorization for 172.4 min. Kinetic studies revealed the Km and kcat values of LacA toward MG were 781.9 mM and 9.5 s-1, respectively. UV–visible spectra confirmed degradation of MG, and the degradation mechanism was explored with liquid chromatography–mass spectrometry (LC-MS) analysis. Based on the LC-MS spectra of degradation products, LacA catalyzed MG degradation via two simultaneous pathways. In addition, the phytotoxicity of MG, in terms of inhibition on seed germination and seedling root elongation of Nicotiana tabacum and Lactuca sativa, was reduced after laccase treatment. These results suggest that laccase of Cerrena was effective in decolorizing MG and promising in bioremediation of wastewater in food and aquaculture industries.
With the development of the Internet of Things (IoTs), widely distributed electronics in the environment require effective in situ energy harvesting technologies, which is made challenging by the unstable supply and severe conditions in some environments. In this work, a hybrid all‐in‐one power source (AoPS) is demonstrated for widely adaptive environmental energy harvesting. With a novel structure, the AoPS hybridizes high‐performance spherical triboelectric nanogenerators (TENGs) with solar cells, enabling the harvesting of most typical environmental energies from wind, rain drops, and sun light, for complementary supply. The spherical TENG units with a packaged structure can work robustly to collect energy from fluid. Nearly continuous direct current and a high average power of 5.63 mW can be obtained by four TENG units, which is further complemented by solar cells. Typical application scenarios are also demonstrated, achieving self‐powered soil moisture control, forest fire prevention and pipeline monitoring. The work realizes the concept of an environmental power source that can be deployed in the environment with high adaptability to make use of all kinds of surrounding energies for powering electronics in all‐weather conditions, providing a reliable foundation for the era of the IoTs.
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