Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora‐L168 and its application in bioremediation of triaryl‐methane dye

Abstract

Laccases are multi copper oxidases that can oxidize both phenolic and nonphenolic lignin related compounds. Consequently, there has been continuous demand for laccases for the oxidative degradation of phenolic dyes in effluents. In view of this, the present work was focused on laccase production by solid substrate fermentation using a newly isolated fungus Perenniporia tephropora‐L168. To intensify the laccase production, the process parameters pH, nitrogen, inducer, and substrate: water ratio were optimized by using statistical model. A set of optimal conditions noted were pH 3, nitrogen 0.001 g/L; inducer 0.5% and substrate: water ratio (1:10), which yielded laccase 1,160 U/g. The crude laccase exhibited noteworthy potential to degrade a triaryl‐methane dye especially Malachite green. Also, during bioremediation studies, the statistical process optimization could achieve 81% decolourization within 180 min. The laccase treatment brought chemical transformation in malachite green as evident from UV–Visible spectra, FTIR, HPLC while toxicity against bacteria and fungi was also reduced. During phytotoxicity study, effect of treated and untreated dye on germination of seed was analyzed. Interestingly, the germination index for Vigna aconitifolia and Vigna radiata was increased by two and fourfold, respectively. Overall, this work demonstrates optimized production of laccase using Perenniporia tephropora‐L168 and its efficient bioremediation potential for triaryl‐methane dye.

Improved laccase production by Trametes versicolor using Copper-Glycyl-L-Histidyl-L-Lysine as a novel and high-efficient inducer

“…Due to the different strains and media, the interaction between the various components of the medium is discrepant, so the optimal medium formulations to obtain the laccase production are diverse. Studies have proved that reasonable optimization of medium components can increase laccase production in fermentation ( Patil et al, 2020 ). In 5 L reactor, the peak of biomass and laccase production was 1 day earlier than that in shake flask, and higher laccase production was obtained.…”