Efficacy of Withania somnifera on seminal plasma metabolites of infertile males: A proton NMR study at 800MHz

Gupta, Ashish; Mahdi, Abbas Ali; Shukla, Kamla Kant; Ahmad, Mohammad Kaleem; Bansal, Navneeta; Sankhwar, Pushplata; Sankhwar, Satya Narayan

doi:10.1016/j.jep.2013.06.024

Cited by 70 publications

(56 citation statements)

References 42 publications

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“…All semen samples were treated following the same protocol in order to assess the sperm parameters, including motility. The seminal plasma was liquefied at 37 C for 30 minutes, and then centrifuged (10 minutes), and finally frozen [Gupta et al 2013, Jayaraman et al 2014. Before the NMR analysis, the samples were lyophilized and resuspended in deuterium oxide.…”

Section: Resultsmentioning

confidence: 99%

“…Before the NMR analysis, the samples were lyophilized and resuspended in deuterium oxide. Figure 1 shows the NMR proton spectrum of a human seminal plasma sample in D 2 O solution acquired at 600 MHz and 298 K. The assignment reported in detail in Table 1 was obtained by bidimensional NMR spectra (data not shown) according to previous papers [Gupta et al, 2011a,b;Averna et al 2005;Gupta et al 2013;Lynch et al 1994;Patel et al 1999;Seaglen et al 1995]. The enlarged signals at 7.18, 6.92, 6.89, and 6.75 ppm, marked with an asterisk in Figure 1, in the region of aromatic protons of phenylalanine, tyrosine, and histidine, show a characteristic line width broadening of proton resonances.…”

Section: Resultsmentioning

confidence: 99%

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Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis

Bonechi

Collodel

Donati

et al. 2015

Systems Biology in Reproductive Medicine

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Human seminal fluid is a complex mixture of secretions originated from epididymis and the male accessory sex glands. It contains a variety of both inorganic and organic components, among which proteins are a major part of the high molecular-mass substances. In this study, 83 human seminal plasma samples were analyzed using a combined Nuclear Magnetic Resonance (NMR) Spectroscopy and Principal Component Analysis (PCA) approach to discriminate patients in relation to semen characteristics and/or conditions affecting the fertility status. Results showed a discrimination between patients with leukocytospermia and with the concomitant presence of varicocele/ex varicocele and leukocytospermia. Patients with testicular cancer, necrozoospermia, and azoospermia were separated from the other patient clusters. In addition, a differentiation of semen quality was also possible. This study represents to first use of sperm parameters together with NMR data as variables in the PCA analysis. Furthermore, this methodology allows the identification of the metabolites which play the most important role in identifying differences among human seminal plasma samples.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis

Bonechi

Collodel

Donati

et al. 2015

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…In the context of phytochemical research, Withania somnifera therapy has been found to repair the disturbed concentrations of lactate, alanine, citrate, GPC, histidine, and phenylalanine in seminal plasma thereby aiding in recovery of semen quality post treatment [14]. Supporting the notion that diabetes effected changes in the metabolome of the testicle itself, the accumulation of advanced glycation end products accumulate in the reproductive tract of men with diabetes [25], and metabolic profile changes in the testes of mice with streptozotocin-induced type 1 diabetes mellitus has been reported [26].…”

Section: Discussionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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Purpose The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. Methods A total of 103 subjects visiting the infertility clinic of Manipal University with normozoospermic parameters, oligozoospermia, asthenozoospermia, azoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria. Seminal plasma was subjected to NMR spectroscopy at a 700 MHz

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“…20 It also repairs the disturbed concentrations of lactate, alanine, citrate, GPC, histidine and phenylalanine in seminal plasma and recovers the quality of semen of post-treated compared to pretreated infertile men. 21 Mucuna pruriens (Kapikachchhu) seeds are rich source of L-DOPA and its metabolites which stimulate the hypothalamus and forebrain to secrete gonadotropin releasing hormone (GnRH) 22 which in turn, upregulates the anterior pituitary gland to secrete FSH and LH causing increased synthesis of testosterone by leydig cells of the testis. 23,24 Mucuna pruriens therapy rectifies the perturbed alanine, citrate, GPC, histidine and phenylalanine content in seminal plasma and improves the semen quality 25 and significantly ameliorated psychological stress by elevating serum cortisol and seminal plasma lipid peroxide level and decreasing seminal plasma glutathione (GSH), ascorbic acid contents and reduced superoxide dismutase (SOD) and catalase activity.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Herbo-Mineral Formulation in the Management of Klaibya (Erectile Dysfunction)

Baruah¹,

Chaudhary²,

Pandey³

2015

Int. J. Res. Ayurveda Pharm.

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Enhanced rate of divorce among most elite couple in metros alarmed a unique social stigma. A man who is full of stresses of life and lost his manhood is accepting his failure and ready to sign paper of divorce in court as an escape route to get rid of his responsibility. Erectile dysfunction (Klaibya) or impotence is emerging as one of the most serious life style and stress related disease. Over exertion, physiological disturbances, lowered level of hormones and strained relationship with partner are the main causes for this disease. The remedial measure of Erectile dysfunction (ED) is still evolving in conventional system of medicine. The search for a perfect aphrodisiac drug specially a herbo-mineral drug that will heighten sexual desire, pleasure and performance has been a continuing cultural quest from ancient era to current times. Therefore, a herbomineral formulation was prepared, which consists of Vanga Bhasma, Ashwagandha (Withania somnifera) Churna and Kapikachhu (Mucuna pruriens) Churna with three Bhavana of each Dhatura (Datura metel) Patra Swarasa and Bhanga (Cannabis sativa) Kwatha to evaluate the clinical effect in 40 male diagnosed patients of Klaibya (Erectile dysfunction). Out of 40 patients Klaibya of 29 completed the full course of trial treatment and 11 patients were dropped out from the study. The result of 29 patients showed statistically significant improvement in some sexual symptoms and seminal parameters.

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Efficacy of Withania somnifera on seminal plasma metabolites of infertile males: A proton NMR study at 800MHz

Cited by 70 publications

References 42 publications

Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis

Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Effect of a Herbo-Mineral Formulation in the Management of Klaibya (Erectile Dysfunction)

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