Efficacy of Platelet-Rich-Plasma (PRP) and Highly Purified Bovine Xenograft (Laddec®) Combination in Bone Regeneration after Cyst Enucleation: Radiological and Histological Evaluation

Abstract

Objectives: The purpose of the present study was to evaluate the efficacy of adding platelet-rich plasma (PRP) to a new highly purified bovine allograft (Laddec ® ) in the bone regeneration of cystic bony defects augmented following cystectomy. Material and Methods: Study sample included 20 patients undergoing cystectomy in which the bone defect was filled with PRP and Laddec ® . All patients were examined with periapical radiographs before operation and at follow-up. After 3 months, at re-entry surgery for implant placement, bone core was taken for histological and histomorphometric analysis. Results: The postoperative successive radiographs showed a good regeneration of bone in the height of bony defects with application of PRP to bone graft. By the first postoperative month, about 48% of the defect was filled, which gradually increased in each month and showed about 90% of defect-fill by 6 months. Histological and histomorphometric analysis, showed a significant presence of bone tissue and vessels, with newly formed bone in contact with anorganic bone particles. The mean volume of vital bone was 68 ± 1.6% and the mean percentage of vital bone was 48 ± 2.4%. The mean percentage of inorganic particles in tissues was 20 ± 1.2% of the total volume. All the samples analyzed did not evidence the presence of inflammatory cells. Conclusions:The results of this study showed how the use of Laddec ® in association with platelet-rich plasma allows bone regeneration and has a potential for routine clinical use for regeneration of cystic bony defects.

Odontoma Removal and Oral Rehabilitation via Insertions of Albumin and Gentamycin Coated Bone Allograft and Dental Implants - A Case Report

“…Bone augmentation and implant insertion is performed later, months or years after tooth removal; however, alveolar preservation may prevent the need of bone replacement [26]. Bone grafting after cystectomy is also a preventive approach so as to facilitate the possibility of oral rehabilitation [27]. This presented case demonstrates that bone grafting can also be an option associated with surgical removal of odontoma.…”

Concise Review: The Use of Adipose-Derived Stromal Vascular Fraction Cells and Platelet Rich Plasma in Regenerative Plastic Surgery

“…PRP was also reported to be effective in stopping capillary bleeding in the surgical flaps of a series of 20 patients undergoing various types of cosmetic surgery (face lift, breast size changes, or neck lifts) . The beneficial use of PRP have also been reported in patients affected by Romberg syndrome as well as in bone regeneration of intrabony defects . However, in attempts to regenerate damaged tissues, one has to keep in mind the risks that can be associated with use of factors that promote epithelial migration, angiogenesis and matrix deposition (e.g., recurrence, growth and invasion of a pre‐existing tumor).…”

Effects of the Autologous Platelet-Rich Plasma in Nasal Pack on Postoperative Quality of Life

“…4 Same doses (2 mL) of PRP or saline (with a 20 Gneedle in sterilized 2.5 mL dental injector) injection has been given to the patients in both groups for the activation of merocel packs. Prior to the start of the surgery, 20 mL of blood was drawn intravenously from patients and collected in sterile vacuum tube coated with anticoagulant citric acid and dextrose.…”