Efficacy of Jatropha curcas Latex Cream in The Epithelialization Phase of Wound Healing in Mice Skin

Abstract

The objective of the present research was to find out the efficacy of Jatropha latex cream in the epithelialization phase of wound healing in mice skin. A total of 9 male mice aged 2-3 months old and the bodyweight of 25-40 g were divided into three treatment groups. Group A was given basic cream, group B was given Jatropha latex cream (10%), and group C was given sulfadiazine. The 2 cm incision wound was made in the paravertebral area. Wound therapy was carried out twice a day for 7 days. All quantitative data were measured using ANOVA then followed by the Duncan test. The number of angiogenesis on A, B, and C was 4.67±1.20; 12.78±2.52; and 11.33±2.33, while a number of fibroblasts were 179.56±12.69; 90.56±8.23; and 99.11±7.04, respectively. The average deposition of collagen was 1.46±0.12; 1.89±0.10; and 1.74±0.06, respectively. The statistical test showed that the number of angiogenesis, fibroblast, and deposition of collagen on group B was significantly different (P<0.05) compared to group A and showed no significant difference compared to group C (P>0.05). In conclusion, Jatropha latex cream (10%) able to accelerate the epithelialization phase of wound healing in mice skin.

The Activity of Jatropha curcas Cream on Day 5 of Skin Wound Healing in Mice Infected with Staphylococcus aureus

“…In this study, an incision was made in the dorsal area (back) of all mice according to Salim et al [16]. The procedure for wound incision was by shaving the hair with a diameter of 3 cm around the area of the skin, and disinfected with 70% alcohol.…”

“…The amount of neovascular in 10% Jatropha cream administration(P3) compared to 0.1% sulfadiazine (P2) was not significantly different (p>0.01). The administration of 10% Jatropha cream (P3) increased the number of neovascularization in the wound area [16,26]. The formation of new blood vessels in the wound area accelerated wound healing [26].…”

Efficacy of Jatropha Sap (Jatropha curcas L.) Cream on Epithelialization Phase of Cutaneous Wound Healing in Mice Using Masson Trichome Staining

“…The flavonoids and saponins contained in the Jatropha's sap stimulate the formation of new epithelial cells and support the epithelialization process so that reduction in wound size has a positive correlation with the re-epithelialization process. The faster the re-epithelialization process, the smaller the wound size will be, thus shortening the wound healing process [3].…”

“…The wound healing process is a complex cellular process that focuses on restoring the integrity of the damaged tissue structure and function through three phases, namely inflammatory, proliferation, and maturation or remodeling phase [3]. Within a few hours after the injury, epithelialization occurs where the epidermal which includes most of the keratin begins to migrate and begins to stratify and differentiate to reconstruct the function of the epidermal barrier [4].…”