The Activity of Jatropha curcas Cream on Day 5 of Skin Wound Healing in Mice Infected with Staphylococcus aureus

Abstract

This research aimed to determine Jatropha sap cream's activity on day 5 of the healing process of skin wounds of mice infected with Staphylococcus aureus. Twenty-seven male mice (Mus musculus) aged 2 months and weighed 30-40 g were used. All mice underwent a 20 mm incision in the dorsal area (back) and infected with 0.2 ml suspension of S. aureus ATCC 25923 PK/5. Treatment of open wounds was carried out twice per day. Group P1 (negative control) was smeared with cream base; group P2 (positive control) smeared with 0.1% sulfadiazine cream; and group P3 was smeared with 10% Jatropha sap cream. Macroscopic observations were carried out for 21 days, while histopathology were observed on 5 th day of healing which performed with hematoxylin-eosin (HE) staining. On day 5, pathological anatomy showed hyperaemic (redness) of the skin wounds, swelling (oedema) of the wound edges and wetness due to exudate. Based on the length of the wound on day 5, it was clear that the effect of 10% Jatropha cream and sulfadiazine cream was better than the base cream. Based on HE staining, P1, P2 and P3 had a very significant effect on the number of inflammatory and neovascularization cell infiltration in the inflammatory phase (p<0.01). The mean of inflammatory cells in P3 was significantly different from P2 and P1 (p<0.01). It can be concluded that 10% Jatropha cream can be used as a topical medicine for infected wounds of mice's skin.