Effects of Torbangun Leaves(Coleus amboinicus Lour) Extract on Blood Glucose and Super Oxide Dismutase Activity in Hyperglycemic Rats

Abstract

This research aimed to analyze the yield of torbangun extract, flavonoid content, and antioxidant activity of TE-S and its effect on the activity of SOD enzymes and blood glucose level in hyperglycemic rats as a DM animal model. The water content of simplicia was measured by the gravimetric method. The antioxidant activity of TE-S was determined using the DPPH method, while the total flavonoid was measured using spectrophotometry. The study was a completely randomized design using 25 Sprague Dawley rats. Rats were divided into four groups, namely NG (negative control, hyperglycemic rats), N (normal rats), HIM (control of metformin drugs 62.5 mg/kg of BW), and HIT (TE-S 620 mg/kg of BW). The treatment was carried out for 14 days. FBG levels were taken on day 0, 4, 7, 11, and 14, measured using a glucometer, while blood serum SOD levels were measured using ELISA. The study showed the water content of torbangun simplicia was 7.99% and TE-S yield from simplicia was 4.69%. TE-S contains total flavonoids of 3.91% and antioxidant activity (IC 50) of 306.28 ppm with a standard of 1 ppm vitamin C. TE-S treatment significantly decreased FBG (p=0.005, α=0.01) and increased SOD levels in hyperglycemic rats. TE-S has the potential to increase blood serum SOD levels by contributing to the availability of antioxidants and decreasing blood glucose levels in hyperglycemic rats.

Taro (Colocasia Esculenta (L.) Schott) Extract didn’t Increase Superoxide Dismutase (SOD) but Decrease C-Reactive Protein (CRP) in Male Wistar Rat (Rattus Norvegicus) Diabetes Mellitus Model

“…The difference in the higher IC50 content (126.4216 mg/L) means it has a lower antioxidant capacity in this study did not give a significant difference in results. Meanwhile, based on the results of this study, Similar to the study conducted by Andrestian et al (2019), in diabetic rats (streptozotocin 40 mg/kgBW) given torbangun leaf extract with an IC50 of 306.28 ppm can increase SOD levels but not significantly for 14 days. Similar to the study conducted by Luandayanti (2013), in diabetic rats induced with streptozotocin 100 mg/kgBW and given mahogany seed methanol extract (IC50 117.73 mg/L) there was an increase in SOD levels but not significant.…”

Protective Effects of the Polyphenolic-Rich Fraction of Cornsilk against Oxidative Stress in Streptozotocin-Induced Diabetic Rats

“…The improved antioxidant enzyme activities demonstrated by the PRF-CS indicated that this fraction might prevent enzymes from glycating or decrease reactive oxygen free radicals and enhance the antioxidant enzyme's activities. The observation demonstrated the free radicals scavenging activity abilities of the PRF-CS, exerting an advantageous action through protection against pathogenic changes generated by superoxide radicals and H2O2 radicals (Andrestian et al 2019). The PRF-CS activities observed might be due to the phenolic compounds.…”

Intervention with Purple Okra Pudding and Supplement to Improve Antioxidant Status in Healthy Adults

“…The treatment group given the purple okra supplement with a quercetin content of 18.65 mg per day showed a decrease in SOD levels of 0.15±0.17 U/mL after the intervention. SOD activity was used as one of the parameters in determining the presence of antioxidant activity (Andrestian et al 2019). The decrease in SOD levels of the subjects given the purple okra supplement can be explained by the role of the supplement in balancing the oxidative stress caused by free radicals.…”