Effects of permethrin on human basophils and lymphocytes in vitro

Diel, F.; Detscher, M.; Borck, H.; Schrimpf, Daniel; Diel, E.; Hoppe, Holger

doi:10.1007/s000110050241

Cited by 17 publications

(12 citation statements)

References 4 publications

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“…However, histamine release is increased significantly in atopics after addition of 10 -5 M PBO alone, and a similar response was observed using the combined agents together with 6.5 ¥ 10 -6 M S-bioallethrin (p < 0.01, Student t-test). In general, and in accordance with previous findings [4,6], S-bioallethrin caused little histamine release from human basophil above the spontaneous response. Total production of lymphokines after 4, 24, 48, and 72 h PHA-stimulated culture period was normalized to be 100% (IL-4: non-atopics = 2 ± 1 pg/ml, atopics = 10 ± 3 pg/ml; IFN-g : non-atopics/atopics < 50 pg/ml).…”

Section: Resultssupporting

confidence: 79%

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Pyrethroids and the synergist piperonyl-butoxide (PBO) affect T-cells and basophils

Diel¹,

Horr²,

Detscher³

et al. 1999

Inflammation Research

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Section: Resultssupporting

confidence: 79%

“…As pyrethroids also induce histamine release in vitro [4], the combined effects of S-bioallethrin and PBO were tested using the HLT with basophils. In Figure 1, it is demonstrated that no significant effect could be observed in cell incubates from the non-atopic volunteers.…”

Section: Resultsmentioning

confidence: 99%

Pyrethroids and the synergist piperonyl-butoxide (PBO) affect T-cells and basophils

Diel¹,

Horr²,

Detscher³

et al. 1999

Inflammation Research

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“…Studies show that PB and PER can modulate both cellmediated and humoral immunity (Peden-Adams et al 2004;Punareewattana et al 2001;Prater et al 2005;Blaylock et al 1995;Diel et al 1998). Our proteomic findings show that immune cell trafficking and inflammatory responses are disturbed in response to GW agents.…”

Section: Discussionmentioning

confidence: 97%

Proteomic CNS Profile of Delayed Cognitive Impairment in Mice Exposed to Gulf War Agents

et al. 2011

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Gulf War Illness (GWI) is a chronic multisymptom condition with a central nervous system (CNS) component, for which there is no treatment available. It is now believed that the combined exposure to Gulf War (GW) agents, including pyridostigmine bromide (PB) and pesticides, such as permethrin (PER), was a key contributor to the etiology of GWI. In this study, a proteomic approach was used to characterize the biomolecular disturbances that accompany neurobehavioral and neuropathological changes associated with combined exposure to PB and PER. Mice acutely exposed to PB and PER over 10 days showed an increase in anxiety-like behavior, psychomotor problems and delayed cognitive impairment compared to control mice that received vehicle only. Proteomic analysis showed changes in proteins associated with lipid metabolism and molecular transport in the brains of GW agent-exposed mice compared to controls. Proteins associated with the endocrine and immune systems were also altered, and dysfunction of these systems is a prominent feature of GWI. The presence of astrogliosis in the GW agent-exposed mice compared to control mice further suggests an immune system imbalance, as is observed in GWI. These studies provide a broad perspective of the molecular disturbances driving the late pathology of this complex illness. Evaluation of the potential role of these biological functions in GWI will be useful in identifying molecular pathways that can be targeted for the development of novel therapeutics against GWI.

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“…DDT has been banned because it is toxic to people and persists in the environment [11,12]. Permethrin can cause eye and skin irritation despite being not very toxic to humans [13]. Malathion is available as a head lice treatment only by prescription after been taken off the market twice in the United States due to flammability and odours [14].…”

Section: Introductionmentioning

confidence: 99%

Genetic Damage and Cell Killing Induction by Five Head Lice Treatments on HaCaT Human Skin Cells

Alnuqaydan¹,

Sanderson²

2016

J Carcinog Mutagen

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Background: Chemical head lice treatments used by parents to treat head lice infestation in their children due to their rapid and reliable removal of head lice. However, those treatments can be absorbed through the skin. Children are more sensitive to absorbing chemicals than adults. We hypothesized that synthetic chemical-based head lice treatments cause cytotoxic and genotoxic damage to human skin cells in vitro. Objective: To determine the cytotoxic and genotoxic damage of synthetic chemical-based head lice treatments on HaCaT human skin cells in vitro.Methodology: Cytotoxicity measured by the methyl tetrazolium cytotoxicity (MTT) assay and the crystal violet assay. Also, the mechanism of cell killing was identified by the apoptosis detection, via Flow cytometry assay. The cytokinesis block micronucleus (CBMN) assay detected the frequency of binucleated cells (BN) with micronucleus (MNi), to indicate genetic damage induced by head lice treatments.Results: Tea Tree Oil (TTO), Pure Lavender oil and Pyrethrum did induce significant cytotoxicity. Also, they enhanced both early apoptosis and late apoptosis/necrosis. However, two head lice treatments, Permethrin (Lice Breaker) and Maldison (Malathion) (KP24) did not induce cytotoxicity. Early apoptosis and necrosis were observed in Permethrin treatment and late apoptosis and early necrosis were measured in Maldison (Malathion) (KP24). Moreover, Permethrin (Lice Breaker) and Maldison (Malathion) (KP24) induced micronuclei (MNi) at a frequency significantly higher (range=15-25 MNi/1000 binucleated cells, n=3) than the background frequency (media alone control; MNi range= 6 MNi /1000 binucleated cells, n=3). Conclusion:This study indicates that exposure to chemical based head lice treatments enhanced cell death by both early apoptosis and late apoptosis/necrosis also induced chromosomal damage in human skin cells.

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Effects of permethrin on human basophils and lymphocytes in vitro

Cited by 17 publications

References 4 publications

Pyrethroids and the synergist piperonyl-butoxide (PBO) affect T-cells and basophils

Pyrethroids and the synergist piperonyl-butoxide (PBO) affect T-cells and basophils

Proteomic CNS Profile of Delayed Cognitive Impairment in Mice Exposed to Gulf War Agents

Genetic Damage and Cell Killing Induction by Five Head Lice Treatments on HaCaT Human Skin Cells

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