Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice

Weitkunat, Karolin; Schumann, Sara; Petzke, K. J.; Blaut, Michaël; Loh, Gunnar; Klaus, Susanne

doi:10.1016/j.jnutbio.2015.03.010

Cited by 174 publications

(157 citation statements)

References 49 publications

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“…This biochemical mechanism will be discussed further in the following paragraph, especially for cholesterol metabolism. The decrease in meat fat mass was consistent with some previous reports (Al-Lahham et al, 2010;Ooi & Liong, 2010;Weitkunat et al, 2015) that the short chain fatty acids (acetate, butyrate, propionate) produced by the fermentative effect of lactic acid bacteria on inulin were assumed to have important contribution. Propionate, for example, could exert controlling effect on lowering hepatic lipogenesis, and the inhibition of lipogenesis process related to the decrease in meat fat content.…”

Section: Level Of Dahlia Tuber Powdersupporting

confidence: 92%

Meat Characteristic of Crossbred Local Chicken Fed Inulin of Dahlia Tuber and Lactobacillus sp.

Abdurrahman¹,

Pramono²,

Suthama³

2016

Med Pet

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This study was conducted to evaluate the meat characteristic of crossbred local chicken fed diet containing both dahlia tuber powder as inulin source and probiotic Lactobacillus sp. The experimental animals were 168 crossbred local chickens which were randomly divided into 6 groups of treatment (4 replications each) when they were 21-d old. A completely randomized design with 2 x 3 factorial pattern consisted of 2 levels of prebiotic [(0.8% (D1) and 1.2% (D2)] and 3 levels of probiotic [without probiotic (L0), 1.2 mL (L1), and 2.4 mL (L2)] was arranged in the present study. One mL probiotic (Lactobacillus sp.) was equal to 10 8 cfu. Results showed that the supplementation of prebiotic and probiotic significantly (P<0.05) affected breast meat color in terms of L* (lightness) and b* (yellowness). The meat fat mass and cholesterol was significantly (P<0.05) decreased by the combination of prebiotic and probiotic. The hardness of meat was not affected significantly by all treatments. The conclusion is that breast meat color could be improved, and both meat fat mass and cholesterol content could be decreased by feeding a combination of 1.2% dahlia tuber powder as inulin source and 1.2 mL probiotic Lactobacillus sp. Key words: prebiotic inulin, probiotic Lactobacillus sp., meat characteristic, crossbred local chicken ABSTRAKTujuan penelitian ini adalah untuk mengevaluasi karakteristik daging ayam lokal persilangan yang diberi ransum mengandung kombinasi prebiotik inulin dari umbi dahlia dan probiotik Lactobacillus sp. Penelitian menggunakan 168 ekor ayam lokal persilangan umur 21 hari yang secara acak ditempatkan pada 6 perlakuan dengan 4 ulangan. Rancangan acak lengkap pola faktorial 2 x 3 diterapkan pada penelitian ini. Penambahan prebiotik sebanyak 0,8% (D1) dan 1,2% (D2) sebagai faktor pertama, dan penambahan probiotik yaitu tanpa probiotik (L0), probiotik 1,2 mL (L1), dan 2,4 mL (L2) sebagai faktor kedua. Satu mililiter probiotik (Lactobacillus sp.) setara dengan 10 8 cfu. Hasil penelitian menunjukkan bahwa penambahan prebiotik dan probiotik pada ransum nyata (P<0,05) mempengaruhi warna daging dada berdasarkan L* (tingkat kecerahan) dan b* (tingkat kekuningan). Massa lemak dan kolesterol nyata (P<0,05) menurun akibat penambahan prebiotik dan probiotik. Kekerasan daging tidak nyata dipengaruhi oleh perlakuan. Kesimpulannya adalah bahwa 1,2% prebiotik dan probiotik 1,2 mL (10 8 cfu/mL) adalah kombinasi yang paling optimum berdasarkan perbaikan warna daging dada dan penurunan massa lemak dan kolesterol daging.

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Section: Level Of Dahlia Tuber Powdersupporting

confidence: 92%

Meat Characteristic of Crossbred Local Chicken Fed Inulin of Dahlia Tuber and Lactobacillus sp.

Abdurrahman¹,

Pramono²,

Suthama³

2016

Med Pet

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“…There is increasing evidence that C17:0 can be biosynthesised in vivo, which could account for its non-dietary origin 24,25 . Several in vitro investigations have suggested that C17:0 is produced endogenously both through elongation of propionyl-CoA 26 and alternatively, through α -oxidation of C18:0 21,27,28 . However, the results for the C17:0 biosynthesis through propionyl-CoA do not directly explain tissue C17:0 levels in whole organisms, since the comparison between the expected and the actual fatty acid levels do not match, which suggests that propionyl-CoA plays a minor role.…”

Section: Discussionmentioning

confidence: 99%

Odd Chain Fatty Acids; New Insights of the Relationship Between the Gut Microbiota, Dietary Intake, Biosynthesis and Glucose Intolerance

Jenkins

Seyssel

Chiu

et al. 2017

Sci Rep

100

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Recent findings have shown an inverse association between circulating C15:0/C17:0 fatty acids with disease risk, therefore, their origin needs to be determined to understanding their role in these pathologies. Through combinations of both animal and human intervention studies, we comprehensively investigated all possible contributions of these fatty acids from the gut-microbiota, the diet, and novel endogenous biosynthesis. Investigations included an intestinal germ-free study and a C15:0/C17:0 diet dose response study. Endogenous production was assessed through: a stearic acid infusion, phytol supplementation, and a Hacl1 −/− mouse model. Two human dietary intervention studies were used to translate the results. Finally, a study comparing baseline C15:0/C17:0 with the prognosis of glucose intolerance. We found that circulating C15:0/C17:0 levels were not influenced by the gut-microbiota. The dose response study showed C15:0 had a linear response, however C17:0 was not directly correlated. The phytol supplementation only decreased C17:0. Stearic acid infusion only increased C17:0. Hacl1 −/− only decreased C17:0. The glucose intolerance study showed only C17:0

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“…The concentrations of SCFA, including acetate, propionate and butyrate in intestinal tract increased in varying degrees after extra addition of slowly digested carbohydrates in daily diets, such as resistant starch, 39) guar gum, 40) pectin, 41) inulin 42) and so on. In this study, acarbose and voglibose significantly increased butyrate concentration (Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparisons of Effects on Intestinal Short-Chain Fatty Acid Concentration after Exposure of Two Glycosidase Inhibitors in Mice

Cai

et al. 2018

Biological & Pharmaceutical Bulletin

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Acarbose and voglibose are the most widely used diabetes drugs as glycosidase inhibitors. In this study, the use of these two inhibitors significantly increased the content of starch in large intestine, and altered the concentration of short-chain fatty acids (SCFAs) by affecting the intestinal microbiota. However, there are some differences in the intestinal microbiome of the two groups of mice, mainly in bacteria such as Bacteroidaceae bacteroides and Desulfovibrionaceae desulfovibrio. The productions of acetate and propionate in caecum in voglibose group were significantly higher than those in acarbose group and two kinds of glycosidase inhibitors were close in the production of butyrate in caecum. The Tax4Fun analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data indicated that different productions of acetate and propionate between acarbose group and voglibose group may be related to 2-oxoisovalerate dehydrogenase and pyruvate oxidase. In addition, in-vitro experiments suggested that voglibose had less effect on epithelial cells than acarbose after direct stimulation. According to the recent researches of SCFAs produced by intestinal microbiota, our comparative study shown higher concentration of these beneficial fatty acids in the lumen of voglibose-treated mice, which implied a lower level of inflammation.Key words acarbose; voglibose; microbiota; short-chain fatty acid; Tax4FunWith the increasing number of obesity and diabetes around the world, there are more and more different classes of medications to treat diabetes, such as biguanides (metformin), sulfonylureas, insulin, glucagon-like peptide 1 (GLP-1) agonists, dipeptidyl peptidase IV inhibitors, thiazolidinediones and sodium-glucose transporter 2 inhibitors (to name a few). 1) However, acarbose and voglibose as part of α-glucosidase inhibitors (AGIs) are another class of anti-diabetic medications. As first-line hypoglycemic drugs used in patients with type 2 diabetes especially in Asian since their diet meals contain high content of carbohydrates, acarbose and voglibose can typically reduce postprandial glucose level by delaying carbohydrate digestion in the gut, so they can be a useful treatment in the patients who raised basal glucose concentrations slightly and marked postprandial hyperglycemia. 2) In addition, there will also be some side effects, mainly reflected in gastrointestinal flatulence, loose stool and so on. The main reason for this is that fermentation of undigested carbohydrates by bacteria produced gas in the colon. 3) Acarbose and voglibose treatment delayed starch digestion in the small intestine but there was compensatory salvage through bacterial fermentation in the large intestine. 4) When carbohydrates (starch, dietary fiber) enter the large intestine, they are degraded by the fermentation of anaerobic flora. 5) Under normal diet situations, the better part of starch was digested and absorbed in the small intestine, therefore, extra starch entering the large bowel changed microbial eco-system to some degree. Som...

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Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice

Cited by 174 publications

References 49 publications

Meat Characteristic of Crossbred Local Chicken Fed Inulin of Dahlia Tuber and Lactobacillus sp.

Meat Characteristic of Crossbred Local Chicken Fed Inulin of Dahlia Tuber and Lactobacillus sp.

Odd Chain Fatty Acids; New Insights of the Relationship Between the Gut Microbiota, Dietary Intake, Biosynthesis and Glucose Intolerance

Comparisons of Effects on Intestinal Short-Chain Fatty Acid Concentration after Exposure of Two Glycosidase Inhibitors in Mice

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