Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage

Bucak, Mustafa Numan; Tuncer, Pürhan Barbaros; Sarıözkan, Savaş; Başpınar, Nuri; Taşpınar, Mehmet; Çoyan, Kenan; Bilgili, Ali; Akalın, Pınar Peker; Büyükleblebici, Serhat; Aydos, Sena; Ilgaz, Seda; Sunguroğlu, Asuman; Öztuna, Derya

doi:10.1016/j.cryobiol.2010.09.001

Cited by 160 publications

(99 citation statements)

References 47 publications

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“…Total abnormalities also decreased in the presence of dithioerithritol and linoleic acid. These results were in agreement with those reported by some researchers, suggesting that antioxidants could decrease the sperm acrosome and total abnormalities for bovine [27,32,33] and for goat [26] . The sperm plasma membrane is sensible to peroxidative damage with accompanying loss of membrane integrity, reduced sperm motility, and finally loss in fertility [34] .…”

Section: Discussionsupporting

confidence: 83%

“…In this study, by the addition of the antioxidants fetuin, dithioerithritol and linoleic acid, DNA integrity was well-kept, compared to the control group. These results were consistent to those reported in a previous research on bovine sperm [32] . On the other hand, the antioxidants which were used had no effects on upgrading at DNA integrity [27] .…”

Section: Discussionsupporting

confidence: 83%

“…Moreover, according to Çoyan et al [31] , the supplementation of dithioerithritol did not improve bull sperm motility during liquid storage. Its declared that, dithioerithritol had no significant improvement in motility characteristics, and CASA and progressive motilities of bovine sperm [32] . After freeze-thawing process, motility analysis did not give enough data for the evaluation of sperm.…”

Section: Discussionmentioning

confidence: 99%

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Brown-Swiss Boğa Spermasının Dondurulmasında Çözündürme Sonrası Mikroskopik ve Oksidatif Stress Parametreleri Üzerine Farklı Antioksidanların Etkileri

BÜYÜKLEBLEBİCİ¹,

BÜYÜKLEBLEBİCİ²,

TAŞDEMİR³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of this study was to research the effects of four antioxidants that had been added to semen diluents, on spermatological parameters, anti-oxidant enzymes activities and DNA integrity after the freeze-thaw procedures in Brown-Swiss bull semen. A total of 24 ejaculates were collected from three bulls. Each ejaculate was divided into five equal parts and they diluted to the Tris + 25 mM trehalose base extender containing fetuin 1 mg/ml, dithioerithritol 1 mM, cysteamine 4 mM or linoleic acid 0.5 ml/50 ml, and no additives (control). Group dithioerithritol and linoleic acid showed the higher rates of CASA progressive motility, CASA sperm motility (P<0.05) compared with the other groups. Group linoleic acid provided the better protective effect for acrosome and total abnormalities (P<0.01, P<0.05; respectively). Also, group dithioerithritol showed the highest values for the HOS test (P<0.01). In the comet test, group dithioerithritol enhanced tail with lesser chromatin damage than the other groups (P<0.05). Supplementation of dithioerithritol significantly affected the GSH activity (P<0.01). Also groups dithioerithritol and linoleic acid gave higher CAT values than the other groups (P<0.05). Additionally, supplementation of fetuin and cysteamine showed the lowest total antioxidant activity value (P<0.05). In conclusion, we may said that, the addition of antioxidants, specially dithioerithritol and linoleic acid, to added with 25 mM trehalose Tris extender improve post-thaw sperm parameters. Keywords: Antioxidants, Catalase, Glutathione peroxidase, Spermatological charecteristics, Bovine Brown-Swiss Boğa Spermasının Dondurulmasında Çözündürme Sonrası Mikroskopik ve Oksidatif Stress Parametreleri Üzerine Farklı Antioksidanların Etkileri ÖzetBu çalışmanın amacı Brown-Swiss boğa spermasında dondurma çözündürme işlemleri sonrasında, spermatolojik parametreleri, antioksidant enzim aktiviteleri ve DNA bütünlüğü üzerine sperma sulandırıcısına eklenen dört antioksidanın etkisini araştırmaktı. Üç boğadan toplam yirmidört ejakülat toplandı. Her bir ejakülat beş eşit kısma ayrıldı ve 25 mM trehaloz katılmış Tris temel sulandırıcısı içerisinde, fetuin 1 mg/ml, dithioerithritol 1 mM, sisteamin 4 mM veya linoleik asit 0.5 ml/50 ml, ve antioksidan içermeyen (kontrol) ile sulandırıldı. Grup dithioerithritol ve L diğer gruplarla kıyaslandığında daha yüksek CASA progressif motilite ve CASA sperma motilitesi gösterdi (P<0.05). Grup linoleik asit akrozom ve toplam abnormalitede daha iyi koruyucu etki sağladı (sırasıyla P<0.01, P<0.05). Keza, grup dithioerithritol, HOS test için en yüksek değeri verdi (P<0.01). Comet testte göre, dithioerithritol grubunda kuyruk kromatin hasarı diğer gruplara göre daha azdı (P<0.05). Dithioerithritol eklenmesi GSH aktivitesini önemli derecede etkiledi (P<0.01). Keza, dithioerithritol ve linoleik asit grupları diğer gruplara göre daha yüksek CAT değeri verdi (P<0.05). Buna ek olarak fetuin ve sisteamin eklenmesi endüşük total antioksidan aktivite değeri gösterdi (P<0.05). Sonuç olarak, 25 m...

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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Brown-Swiss Boğa Spermasının Dondurulmasında Çözündürme Sonrası Mikroskopik ve Oksidatif Stress Parametreleri Üzerine Farklı Antioksidanların Etkileri

BÜYÜKLEBLEBİCİ¹,

BÜYÜKLEBLEBİCİ²,

TAŞDEMİR³

et al. 2015

Kafkas Univ Vet Fak Derg

Self Cite

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“…It is widely acknowledged that the cryo-and oxidative damage resulting from freezing and thawing may lead to a decline in sperm motility, viability and membrane fluidity (Ball 2008;Bucak et al 2010). Sperm cryopreservation has been repeatedly associated with axonemal alterations, leading to disruptions to sperm motion behaviour and mitochondrial membrane potential, ATP depletion, premature acrosome reaction and increased morphological alterations (de Lamirande and Gagnon 1992;Ball 2008).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Tvrdá¹,

Mackovich²,

Greifová³

et al. 2017

Vet. Med. - Czech

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Reactive oxygen species overgeneration as a side effect of semen cryopreservation may lead to lipid peroxidation, protein degradation, DNA fragmentation and cell death, resulting in a decrease of sperm survival and fertilisation ability. Lycopene has been proposed as a potential supplement to semen extenders because of its antioxidant properties. The aim of this study was to evaluate the effects of lycopene on the structural integrity, functional activity and selected oxidative stress parameters of cryopreserved bovine sperm. Thirty bovine ejaculates were split into two aliquots and diluted with a commercial semen extender supplemented with 1.5 mmol/l lycopene or containing no supplement (control), cooled down to 4 °C, frozen and kept in liquid nitrogen. Prior to experiments, frozen straws were thawed at 37 °C for 20 s. Lycopene addition resulted in a higher sperm motility (P < 0.001), progressive motility (P < 0.001) and all secondary motion characteristics (P < 0.001 with respect to the average path velocity, linear velocity, velocity of curvilinear motion, beat cross frequency, path straightness and linearity; P < 0.01 in the case of the amplitude of lateral head displacement). Furthermore, lycopene exhibited protective effects on the sperm membrane (P < 0.05) and acrosomal (P < 0.01) integrity in comparison to control. An assay for metabolic function revealed that lycopene supplementation to the cryopreservation medium resulted in a higher preservation of the sperm mitochondrial activity (P < 0.001). Reactive oxygen species production as well as intracellular superoxide generation were decreased following lycopene addition (P < 0.01 in the case of reactive oxygen species; P < 0.001 with respect to superoxide production). Finally, the presence of lycopene led to a decrease in protein carbonyl production (P < 0.01), lipid peroxidation (P < 0.001) as well as oxidative DNA damage (P < 0.05) when compared to control. In conclusion, lycopene exhibited significant reactive oxygen species-trapping and antioxidant properties which may prevent oxidative damage to frozen-thawed sperm, and, thus, enhance the post-thaw vitality of male reproductive cells in cattle breeding.

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“…The use of TCM-199, supplemented with 10% foetal bovine serum, pyruvate, follicle-stimulating hormone, estradiol, and antibiotics, remains the method used in most laboratories to culture mature bovine embryos (Sirard et al, 1988). Frozen-thawed bull semen diluted in TCM-199 supplemented with antioxidants did not show significant improvement in sperm motility characteristics (Bucak et al, 2010;Sarıözkan et al, 2014), but increased post-thaw sperm acrosome damage and abnormalities (Sarıözkan et al, 2014). To the knowledge of the authors, there is no study available, to date, on the liquid preservation of freshly collected bull semen using TCM-199 or Ham's F10 culture media.…”

Section: Introductionmentioning

confidence: 99%

Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

Raseona¹,

Ajao²,

Nethengwe³

et al. 2017

SA J. An. Sci.

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The aim of this study was to evaluate the viability of bull spermatozoa diluted with commercial semen extender and two culture media stored at controlled room temperature (24 °C) for 72 hours. Two Nguni bulls were used for semen collection with the aid of an electro-ejaculator. After macroscopic evaluation, semen was pooled and aliquoted randomly into Triladyl, modified Ham's F10, and TCM-199 culture media, and then stored at 24 °C. Sperm motility parameters, morphology, and viability were analysed with computer aided sperm analysis (CASA) after 0, 24, 48 and 72 hours. The study was replicated four times, and data were analysed using analysis of variance (ANOVA). Triladyl had significantly higher sperm viability rate (41.3%) and total motility rate (96.3%) for 72 hours than modified Ham's F10 (86.8%; 26.5%) and TCM-199 (76.7%; 25.0%) culture media. Ham's F10 had higher progressive motility rate (37.8%) than the other extenders TCM-199 (31.7%) and Triladyl (23.4%). There was no significant difference in viability rate between Ham's F10 (26.5%) and TCM-199 (25.0 %) after 72 hours' storage at 24 °C. Furthermore, no significant difference was observed in total sperm abnormalities, except for reacted acrosomes and absent tails, between the two Nguni bulls. Nguni semen can be preserved in Triladyl or modified Ham's F10 and TCM-199 culture media, stored at 24 °C, and stay viable for 72 hours.

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Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage

Cited by 160 publications

References 47 publications

Brown-Swiss Boğa Spermasının Dondurulmasında Çözündürme Sonrası Mikroskopik ve Oksidatif Stress Parametreleri Üzerine Farklı Antioksidanların Etkileri

Brown-Swiss Boğa Spermasının Dondurulmasında Çözündürme Sonrası Mikroskopik ve Oksidatif Stress Parametreleri Üzerine Farklı Antioksidanların Etkileri

Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

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