Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Tvrdá, Eva; Mackovich, Alica; Greifová, Hana; Hashim, Faridullah; Lukáč, Norbert

doi:10.17221/86/2017-vetmed

Cited by 27 publications

(29 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tvrda, Mackovich [20] suggested that lycopene supplementation to semen extender acts as an In vivo result showed that watermelon in dextrose saline when used as diluent for rooster semen enhanced fertility and hatchability of eggs than undiluted semen and dextrose saline.. This thus corroborates claim by Jimoh and Ayedun [13] that watermelon juice is a potent diluent and can be incorporated as extender constituent in the preservation of semen for optimal performance.…”

Section: Discussionsupporting

confidence: 63%

Potential of watermelon (Citrullus lanatus) to maintain oxidative stability of rooster semen for artificial insemination

Jimoh¹,

Akinola²,

Oyeyemi³

et al. 2021

J Anim Sci Technol

View full text Add to dashboard Cite

Fruits with antioxidant enrichment can be an economically affordable supplement for mitigating oxidative damage prone spermatozoa membrane pathologies. Computer-assisted sperm analyzer and oxidative status were utilized to evaluate the impact of watermelon ( Citrullus lanatus ) fortification of dextrose saline as diluent for rooster semen and fertility response of hens inseminated. Watermelon juice and dextrose saline were used to formulate diluent of 7 treatments consisting of unextended semen (positive control), 10%, 20%, 30%, 40%, 50% and only dextrose saline (negative control) designated as Treatments 1–7. Pooled semen was obtained from fertile roosters and equilibrated with diluents at ratio 1:2 in the various treatments and were evaluated using computer software coupled microscope and seminal oxidative status assay. 168 laying hens randomly divided into 7 treatment of 8 replicates and 3 hen per replicate. Hen were everted, and semen (2 × 10 8 Spermatozoa) deposited intra-vagina and eggs collected over 8 weeks to assess fertility and hatchability of eggs laid. The result obtained revealed that watermelon-dextrose saline rooster semen diluent enhanced progressive motility, sperm kinetics and lowered non-progressive motility in T2–T6 compared to T7 over the 3 hours of evaluation. Watermelon addition to rooster semen diluent enhance the antioxidant capacity of rooster semen and lowered lipid peroxide generation. The percentage fertility was highest in T3 (81.01%) and T4 (81.24%) with lowest value obtained in T7 (73.46%). The hatchability of eggs set of hens inseminated with undiluted semen (71.46%) was lower than values for hens inseminated with watermelon inclusive extended semen (75.71%–80.39%). The optimal inclusion of 30%–40% watermelon in dextrose saline diluent enhance rooster semen kinetics, seminal oxidative stability and egg fertility.

show abstract

Section: Discussionsupporting

confidence: 63%

Potential of watermelon (Citrullus lanatus) to maintain oxidative stability of rooster semen for artificial insemination

Jimoh¹,

Akinola²,

Oyeyemi³

et al. 2021

J Anim Sci Technol

View full text Add to dashboard Cite

show abstract

“…Acrosomal integrity was evaluated using bright field microscopy at 1000× using oil immersion. At least 200 cells per slide were evaluated for the presence or absence of acrosome, and expressed as a percentage rate (Tvrda et al 2017).…”

Section: Acrosomal Integritymentioning

confidence: 99%

Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Halo

Massányi²,

Greń³

et al. 2019

Physiol Res

View full text Add to dashboard Cite

The target of this study was to evaluate the effect of extract of the European mistletoe -Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system was used to determine the spermatozoa motility parameters at different time intervals (0, 1, 2 and 3 h) and spermatozoa viability was determined in five different doses of Viscum album quercus L [10 (QA), 6.6 (QB), 3.3 (QC), 2.5 (QD) and 2 (QE) mg/ml]. Results in experimental groups detected a significant deterioration on rabbit spermatozoa after 1, 2 and 3 hours, compared to the control. The initial total spermatozoa motility showed increased value for all doses of Viscum album quercus in comparison to control. After in vitro culture a dose-dependent decrease (QA: reduction of 69.7 %, QB: reduction of 40.9 %) was found. For the progressive spermatozoa most significant decrease (86.8 % for QA vs. 48.5 % for QB) was detected compared to the control after 3 hours of culture. Spermatozoa viability (MTT test) was decreased in all experiment groups at the end of experiment, but the differences were not significant.Significant alterations of membrane integrity were found in groups with the highest Viscum album quercus concentration (QA, QB), but acrosome integrity showed no significant changes. Results suggest negative dose-and time-dependent effect ofViscum album quercus at higher doses on spermatozoa motility and viability parameters in vitro.

show abstract

“…In more recent studies, lycopene decreased lipid peroxidation of spermatozoa and increased sperm viability and motility [12]. Via antioxidant mechanisms, leptin, carnitine, and lycopene reduced DNA damage during storage of spermatozoa [12] [13] [14]. Natural antioxidants, such as ascorbic acid and α-tocopherol, have produced conflicting results on sperm function post-preservation [7] [8] [15] [16], suggesting there may be an optimal threshold for antioxidant supplementation, and above that threshold, the beneficial effects may not be realized.…”

Section: Introductionmentioning

confidence: 92%

“…Prevention of oxidative stress by supplementation of culture media and cryopreservation extenders with antioxidants has been investigated previously [9] [10] [11]. In more recent studies, lycopene decreased lipid peroxidation of spermatozoa and increased sperm viability and motility [12]. Via antioxidant mechanisms, leptin, carnitine, and lycopene reduced DNA damage during storage of spermatozoa [12] [13] [14].…”

Section: Introductionmentioning

confidence: 99%

Bovine Sperm Motility as Affected by Alpha Tocopherol and Ascorbic Acid during Storage

Page¹,

Rosenkrans²

2019

ARSci

View full text Add to dashboard Cite

Our purpose was to determine the impact of ascorbic acid and α-tocopherol on bovine sperm motility following short-term storage or cryopreservation. Semen was collected from mature Angus bulls (n = 4). The experimental design was a randomized complete block, with bull serving as block, and treatments were structured as a 4 × 4 factorial with four concentrations of ascorbic acid (0, 5, 10, 20 mM) and four concentrations of α-tocopherol (0, 0.05, 0.5, 5 mM). Sperm motility characteristics were evaluated using computer assisted sperm analyses at 0, 4, and 8 h of incubation (39˚C) and post-cryopreservation. Initial sperm motility was (79.6% ± 1.6) and decreased to (6.1% ± 1.6%) after cryopreservation. Cryopreserved spermatozoa had lower (P < 0.05) post-thaw qualities when compared with fresh collections and for spermatozoa that had been incubated for 4 h at 39˚C. In contrast, most of the motility characteristics of spermatozoa that were incubated for 8 h at 39˚C were similar to those of post-thaw spermatozoa. Sperm motility, hyperactivity, and velocity characteristics were not affected (P > 0.1) by α-tocopherol. Addition of 20 mM ascorbic acid to storage media decreased (P < 0.05) sperm velocity traits, but the addition of 5 or 10 mM ascorbic acid did not alter sperm velocity. Sperm cell oxidation following cryopreservation/post-thaw was affected by an interaction (P < 0.05) between concentrations of ascorbic acid and α-tocopherol. Stepwise regression models predicted (P < 0.05) post-thaw motility and velocity characteristics of cryopreserved spermatozoa. Our results suggest that adding ascorbic acid and α-tocopherol was not beneficial for short-term storage of spermatozoa; however, our results were inconclusive with regards to inclusion of ascorbic acid and α-tocopherol in egg yolk-based cryopreservation media.

show abstract

Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Cited by 27 publications

References 33 publications

Potential of watermelon (Citrullus lanatus) to maintain oxidative stability of rooster semen for artificial insemination

Potential of watermelon (Citrullus lanatus) to maintain oxidative stability of rooster semen for artificial insemination

Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Bovine Sperm Motility as Affected by Alpha Tocopherol and Ascorbic Acid during Storage

Contact Info

Product

Resources

About