Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Halo, Marko; Massányi, Peter; Greń, Agnieszka; Lasak, A; Slanina, Tomáš; Ondruška, Ľubomír; Muchacka, Renata; Galbavy, D; Ivanič, Peter; Er, Schneir; Formicki, Grzegorz

doi:10.33549/physiolres.934223

Cited by 13 publications

(7 citation statements)

References 55 publications

(30 reference statements)

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“…Many studies indicate the efficacy of the extract of VA [32,33] and some were performed using clinical evaluation of homeopathic VA [34]. In the current study we used the ultradiluted form of the VA, not the extract, and no adverse effects were observed, either through clinical observation or in hematological exams, corroborating the findings of other studies with VA [14,30,34].…”

Section: Discussionsupporting

confidence: 84%

Safety Assessment of Intravenous Application of the Homeopathic Medicine Viscum Album D3 in Dogs

Valle¹,

Aguiar²,

Brunel³

et al. 2021

Asian J Complement Altern Med

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Background: Viscum album (VA) extracts have a wide variety of biologically active compounds that can be used in the treatment of various diseases, with cancer being the main one. However, few data on the effects of homeopathic VA are found in the literature, even though commercially VA formulations are available, with extremely low doses of its active compounds. Previous studies used the anthroposophical form of Viscum and provided information on the mechanisms of action of this drug in tumor cells or in cells of the immune system. Materials and methods: This study aimed to evaluate the safety of intravenous and subcutaneous application of D3 homeopathic Viscum album (VAD3) in dogs. Healthy dogs (n=7) underwent blood collection for blood cells count and biochemistry analysis and clinical examinations 7 days prior and at days 0, 3, 5, 7, 10, 12, and 14 after the beginning of treatment. The dogs received intravenous application of VAD3 at days 0, 5, 10, and 14, and subcutaneous at days 3, 7, and 12. Results: There was a transient increase in the number of monocytes (P=0.0022) at day 5, while there were no significant (P>0.05) changes in any of the other blood endpoints over time. Nevertheless, all blood parameters remained within the reference values for the species. All animals completed the study in good health conditions. According to the Quality-of-Life Assessment Questionnaire, all animals showed improvement in mood and appetite after receiving VAD3. Conclusion: The homeopathic medicine VAD3 is safe for intravenous and subcutaneous applications in dogs, besides possibly bringing benefits to the entire organism.

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Section: Discussionsupporting

confidence: 84%

Safety Assessment of Intravenous Application of the Homeopathic Medicine Viscum Album D3 in Dogs

Valle¹,

Aguiar²,

Brunel³

et al. 2021

Asian J Complement Altern Med

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show abstract

“…Thus, it is well-known fact that there is an action of the components of the VA extract on cancer cells [11,18,19]. However, the literature still does not present data about the in vitro evaluation of homoeopathic VA, with only 7 articles in Pubmed database using the terms "Viscum album and homeopathic" (in May-2020) which are of especial relevance due to the low frequency of occurrence of side effects produced by homoeopathic formulations.…”

Section: Discussionmentioning

confidence: 99%

Homoeopathic Viscum album (10-3) is More Cytotoxic to in vitro Culture of Human Breast Cancer Cell Line than to Human Mesenchymal Stem Cells

Valle¹,

Aguiar²,

Brunel³

et al. 2021

Int J High Dilution Res

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BACKGROUND Adenocarcinomas can be of several types, and MCF-7 is an adenocarcinoma of human breast cell line useful as preclinical model to screen therapeutic agents such as ultra-diluted Viscum album, an European plant whose extract is commonly used in cancer therapy. AIMS MCF-7 and mesenchymal stem cells were used to evaluate the in vitro cytotoxicity of homoeopathic Viscum album 1x10-3 (VAD3). METHODS cells were cultured for 24 hours in controlled environment (37.5oC and 5% CO2) in 96-well plates. After this time, VAD3 was added to the culture medium in concentrations varying from 10 to 100 ?L/mL for MTT assay (evaluation of viability of cells). A control group was maintained with culture medium only. After 48 hours, the procedures of analysis of cells viability were performed. RESULTS MTT assay showed that the concentrations of 42 ?L/mL and 62 ?L/mL were able to reduce cell viability to 50% in MCF-7 and mesenchymal stem cells, respectively, which means that half of the cells cultured were dead after 48 hours in contact with VAD3. CONCLUSION Viscum album presented higher cytotoxic action on human breast cancer cell line culture than on mesenchymal stem cells. This medicine is extensively used against cancer, and the use of the homoeopathic form of it brings new possibilities as no or fewer adverse effects would be present.

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“…The extract of this parasitic plant has been added to rabbit semen during incubation at 37 °C for 3 h [ 85 ]. The range of concentrations used was from 2 to 10 mg/mL.…”

Section: Herbal Pharmacognosy and Its Use In Andrologymentioning

confidence: 99%

Plant Extracts as Alternative Additives for Sperm Preservation

Ros-Santaella

Pintus

2021

Antioxidants

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Sperm preservation is a crucial factor for the success of assisted reproductive technology (ART) in humans, livestock, and wildlife. Irrespective of the extender and the storage conditions used, semen handling and preservation negatively affect sperm quality. Moreover, oxidative stress, which often arises during semen storage, significantly reduces sperm function and compromises the sperm fertilizing ability by inducing oxidative damage to proteins, lipids, and nucleic acids. Plant extracts have recently emerged as a cheap and natural source of additives to preserve and enhance sperm function during semen storage. The present work provides an update on the use of these natural compounds as alternative additives for sperm preservation in 13 animal species, including humans. A detailed description of the effects of 45 plant species, belonging to 28 families, on sperm function during semen storage is presented. The plant material and extraction method employed, dosage, possible toxic effects, and antimicrobial properties are provided.

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Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Cited by 13 publications

References 55 publications

Safety Assessment of Intravenous Application of the Homeopathic Medicine Viscum Album D3 in Dogs

Safety Assessment of Intravenous Application of the Homeopathic Medicine Viscum Album D3 in Dogs

Homoeopathic Viscum album (10-3) is More Cytotoxic to in vitro Culture of Human Breast Cancer Cell Line than to Human Mesenchymal Stem Cells

Plant Extracts as Alternative Additives for Sperm Preservation

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