Plant Extracts as Alternative Additives for Sperm Preservation

Ros-Santaella, José Luis; Pintus, Eliana

doi:10.3390/antiox10050772

Cited by 30 publications

(19 citation statements)

References 91 publications

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“…Moreover, alkaloids and Vit E were found in WNAE. Alkaloids were beneficial for sperm preservation [ 34 ]. Therefore, WNAE has the potential to effectively scavenge free radicals and increase sperm quality in the vitro model more than Vit E, due to the three compounds of WNAE in the form of crude extract being more effective than isolated compounds [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Laoung-on

Jaikang

Saenphet

et al. 2022

Plants

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The white Nelumbo nucifera petals aqueous extraction (NAE) was prominent in phytochemical content, antioxidant activity, and enhanced rat sperm viability induced by FeSO4, a heavy metal. Mancozeb (MZ) contains heavy metals and is widely used for fungal control in agriculture and industry. It induces oxidative stress and causes of spermatogenesis and reproductive organs’ abnormalities in both humans and animals. The aims of the present study were to investigate the effects of white Nelumbo nucifera petals aqueous extraction (WNAE) on sperm quality in cattle sperm induced by MZ. Moreover, this study investigated phytochemical compounds by liquid chromatography-mass spectrometry. A protein profile related to sperm quality with SDS-page and sperm energy preservation for each treatment was determined. The results found nine phytochemical compounds, in which quercetin-3-O-arabinoglycoside was a major flavonoid that was found in the WNAE. MZ induced free radicals in cells, leading to LPO and protein oxidation, while decreasing sperm motility, sperm viability, acrosome integrity, and normal sperm morphology. The cattle sperm found four proteins related to sperm quality including MWs of 17, 31, 34, and 55 kDa. The WNAE effectively increased energy preservation, sperm motility, sperm viability, acrosome integrity, and normal sperm morphology. The WNAE enhanced sperm qualities by reducing oxidative stress. It might be suggested that WNAE has benefits for sperm preservation which may be used to guard against toxicity in animals or humans exposed to MZ contaminants.

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Section: Discussionmentioning

confidence: 99%

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Laoung-on

Jaikang

Saenphet

et al. 2022

Plants

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“…In other studies, egg yolk significantly improved the parameters of red deer epididymal spermatozoa after dilution and during storage in a liquid state [ 45 , 47 ]. However, egg yolk can lead to microbiological contamination of preserved sperm [ 48 ], which is why plant-based substitutes are increasingly used to store human and animal semen [ 49 ]. In extenders, egg yolk can be replaced by soy lecithin (a component of BioXcell ® ), lipoproteins and plant proteins (components of BoviFree ® ).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C

Dziekońska

Neuman

Burdal

et al. 2022

Animals

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The aim of this study was to evaluate the effect of different extenders on the quality of European red deer epididymal sperm stored at 5 °C. Epididymal spermatozoa were collected post mortem from 10 stags and diluted with three extenders (Bovidyl®, BoviFree®, and BioXcell®) and stored at 5 °C. Sperm motility (TMOT), motility parameters (system CASA), plasma membrane integrity (SYBR-14+/PI−), acrosomal membrane integrity (FITC-PNA−/PI−), mitochondrial activity (JC-1/PI), viability, and apoptotic-like changes (YOPRO/PI) were evaluated. The analyses were conducted on the first and successive days of storage (D1–D7). The applied extender, storage time, and the interactions between these factors significantly (p < 0.001) affected most of the analyzed parameters whose values were highest in sperm samples stored in Bovidyl®, regardless of storage time. In Bovidyl®, BoviFree®, and BioXcell® extenders, TMOT values were estimated at 83%, 63%, and 59%, respectively, on D3. The extenders significantly influenced DNA integrity on D7. The percentage of dead sperm increased from D3. The quality of stored sperm cells was significantly influenced by the extenders’ biochemical composition. BoviFree® and BioXcell® contain glycerol which could contribute to deteriorating the quality of spermatozoa stored at 5 °C. Sperm cells stored in the egg yolk-based extender (Bovidyl®) were characterized by the highest viability and functionality.

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“…Antioxidants evidently provide a cryoprotective effect to spermatozoa of different species, including humans (for a review, see Ros-Santaella & Pintus, 2021). In this study, we supplement a Tris-egg yolk extender with crocin, a known potent antioxidant, foreseeing an improvement of post-thaw semen parameters.…”

Section: Discussionmentioning

confidence: 99%

“…The structural and functional integrity of post‐thaw spermatozoa is a prerequisite for successful in vivo and in vitro fertilization (Gillan et al., 2005). Antioxidants evidently provide a cryoprotective effect to spermatozoa of different species, including humans (for a review, see Ros‐Santaella & Pintus, 2021). In this study, we supplement a Tris‐egg yolk extender with crocin, a known potent antioxidant, foreseeing an improvement of post‐thaw semen parameters.…”

Section: Discussionmentioning

confidence: 99%

The addition of crocin in the freezing medium extender improves post‐thaw semen quality

Sapanidou

Lavrentiadou

Errico³

et al. 2021

Reprod Domestic Animals

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Semen cryopreservation is arguably the most important method or technique contributing to the advancement of modern animal production. However, the quality of sperm after thawing is still highly variable. The addition of antioxidant compounds to the freezing medium has been used customarily to counteract the harmful effects of Reactive Oxygen Species (ROS) that are produced during the freeze/thaw process.Crocin, a potent antioxidant, improves the fertilizing capacity of spermatozoa. In this study, we evaluated the potential of crocin (0, 0.5 and 1 mM) as an extender additive to diminish the damaging effects of cryopreservation on bovine spermatozoa. Postthaw semen quality was assessed by means of motility, viability and lipid peroxidation (LPO). We further investigated the effect of crocin supplementation upon freezing on sperm quality parameters during their incubation at 37°C for up to 2 hr. Overall, the

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Plant Extracts as Alternative Additives for Sperm Preservation

Cited by 30 publications

References 91 publications

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C

The addition of crocin in the freezing medium extender improves post‐thaw semen quality

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